LOCUS       sequence-189185-        3243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for NKX2-5 Y191C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pENTR-2B-Nkx2-5 Y191C
FEATURES             Location/Qualifiers
     source          1..3243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1115..1214)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1326..1345)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1337..2146
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1404..1423)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2236..2824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2725..2744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2989..3075
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3167..3194
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3202..3225
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt
      121 cgactggatc cgaccatgtt ccccagccct gcgctcacac ccacgccttt ctcagtcaaa
      181 gacatcctga acctggagca gcagcagcgt agcctggcgt ctggggacct gtctgcgcgc
      241 ctcgaggcca ccctggcccc tgcctcctgc atgctggccg ccttcaagcc cgaggcctac
      301 tctggccccg aggcggcagc gtccggcctg gcagagctgc gcgcggagat gggccccgcg
      361 ccttcgcccc ccaagtgctc tcctgctttc ccagccgccc ccacatttta cccgggagcc
      421 tacggtgacc ctgacccagc caaagaccct cgggcggata aaaaagagct gtgcgcgctg
      481 cagaaggcag tggagctgga caaagccgag acggatggcg ccgagagacc acgcgcacgg
      541 cggcgacgga agccacgcgt gctcttctcg caggcgcagg tctacgagct ggagcggcgc
      601 ttcaagcaac agcggtacct gtcggcgcca gagcgcgacc agctggccag cgtgctgaag
      661 ctcacgtcca cgcaggtcaa gatctggttc cagaaccgtc gctgcaagtg caagcgacag
      721 cggcaggacc agactctgga gcttctgggg ccgccgccgc cgcccgcgcg caggatcgcg
      781 gtgcccgtgc tggtgcgcga cgggaagccc tgcctggggg accccgcggc ctacgctccc
      841 gcctacggcg tgggtctcaa tgcctatggc tacaacgcct acccctaccc cagctacggc
      901 ggcgcggcct gcagtcccgg ctacagctgc gccgcctacc ccgctgcgcc ccccgccgcg
      961 cagccccccg ccgcctccgc caacagcaac ttcgtgaact ttggcgtcgg ggacttgaac
     1021 accgtgcaga gtcccgggat gccgcagggc aattcgggcg tctccacgct gcacggcatc
     1081 cgagcctggt cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagttggc
     1141 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1201 aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta
     1261 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1321 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1381 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1441 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1501 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1561 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1621 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1681 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1741 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1801 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1861 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1921 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1981 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     2041 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     2101 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     2161 ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc ccgtagaaaa
     2221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2881 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ctagcatgga
     2941 tctcggggac gtctaactac taagcgagag tagggaactg ccaggcatca aataaaacga
     3001 aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3061 ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg
     3121 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg
     3181 acggatggcc tttttgcgtt tctacaaact cttcctgtta gttagttact taagctcggg
     3241 ccc
//
