LOCUS       sequence-189186-        3609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for Gata4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pENTR-2B-Gata4
FEATURES             Location/Qualifiers
     source          1..3609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1481..1580)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1692..1711)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1703..2512
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1770..1789)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2602..3190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3091..3110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3355..3441
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3533..3560
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3568..3591
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt
      121 cgactggatc cggtaccgac catgtaccaa agcctggcca tggccgccaa ccacggcccc
      181 ccgcccggcg cctacgaagc aggtggccct ggcgccttca tgcacagcgc gggcgccgcg
      241 tcctcgcccg tctacgtgcc cactccgcgg gtgccgtcct ctgtgctggg cctgtcctac
      301 ctgcagggcg gtggcagtgc cgctgcagct ggaaccacct cgggtggcag ctccggggcc
      361 ggcccgtcgg gtgcagggcc tgggacccag cagggtagcc ctggctggag ccaagctgga
      421 gccgagggag ccgcctacac cccgccgccc gtgtccccgc gcttctcttt cccggggact
      481 actgggtccc tggcggccgc tgccgccgct gccgcagccc gggaagctgc agcctacggc
      541 agtggcggcg gggcggcggg cgctggtctg gctggccgag agcagtacgg gcgtccgggc
      601 ttcgccggct cctactccag cccctaccca gcctacatgg ccgacgtggg agcatcctgg
      661 gccgcagccg ctgccgcctc tgccggcccc ttcgacagcc cagtcctgca cagcctgcct
      721 ggacgggcca accccggaag acaccccaat ctcgatatgt ttgatgactt ctcagaaggc
      781 agagagtgtg tcaattgtgg ggccatgtcc accccactct ggaggcgaga tgggacggga
      841 cactacctgt gcaatgcctg tggcctctat cacaagatga acggcatcaa ccggcccctc
      901 attaagcctc agcgccgcct gtccgcttcc cgccgggtag gcctctcctg tgccaactgc
      961 cagactacca ccaccacgct gtggcgtcgt aatgccgagg gtgagcctgt atgtaatgcc
     1021 tgcggcctct acatgaagct ccatggggtt cccaggcctc ttgcaatgcg gaaggagggg
     1081 attcaaacca gaaaacggaa gcccaagaac ctgaataaat ctaagacgcc agcaggtcct
     1141 gctggtgaga ccctccctcc ctccagtggt gcctccagcg gtaactccag caatgccact
     1201 agcagcagca gcagcagtga agagatgcgc cccatcaaga cagagcccgg gctgtcatct
     1261 cactatgggc acagcagctc catgtcccag acattcagta ctgtgtccgg ccacgggccc
     1321 tccatccatc cagtgctgtc tgctctgaag ctgtccccac aaggctatgc atctcctgtc
     1381 actcagacat cgcaggccag ctccaagcag gactcttgga acagcctggt cctggctgac
     1441 agtcatgggg acataatcac cgcgcactcg agatatctag acccagcttt cttgtacaaa
     1501 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1561 caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg
     1621 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1681 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     1741 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1801 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1861 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     1921 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     1981 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     2041 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2101 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2161 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2221 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2281 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2341 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2401 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2461 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2521 ggttaattgg ttgtaacatt attcagattg ggccccgttc cactgagcgt cagaccccgt
     2581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3241 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag
     3301 catggatctc ggggacgtct aactactaag cgagagtagg gaactgccag gcatcaaata
     3361 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     3421 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
     3481 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc
     3541 atcctgacgg atggcctttt tgcgtttcta caaactcttc ctgttagtta gttacttaag
     3601 ctcgggccc
//
