LOCUS       sequence-189187-        3840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector TBX5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pENTR-2B-Tbx5
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1712..1811)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1923..1942)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1934..2743
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2001..2020)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2833..3421
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3322..3341
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3586..3672
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3764..3791
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3799..3822
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt
      121 cgactggatc cggtaccgac catggccgat acagatgagg gctttggcct ggcgcgcacg
      181 cctctggagc ctgattccaa agacaggtct tgcgattcga aacctgagag tgctctgggg
      241 gctcccagca agtctccatc atccccgcag gctgccttca cccagcaggg catggaagga
      301 atcaaggtgt ttcttcatga acgtgaactg tggctgaagt tccacgaagt gggcacagag
      361 atgatcatca ccaaggcagg gaggagaatg tttcctagtt acaaagtgaa ggtgactggc
      421 cttaatccca aaacgaagta tattcttctc atggatattg ttcccgcaga cgaccacaga
      481 tataaatttg ctgataacaa atggtccgta actggcaaag cagagcctgc catgccgggg
      541 cgcctttacg tgcacccgga ctccccagca accggagccc actggatgcg acaacttgtc
      601 tccttccaga agctcaaact caccaacaac cacctggacc cgtttggaca cattatcctg
      661 aactccatgc acaaatacca gccccgatta cacatcgtga aagcagacga aaataatggg
      721 ttcggttcaa agaacactgc gttttgcacc cacgtcttcc cggagacagc ttttatcgct
      781 gtgacttcgt accagaatca caagatcaca cagctgaaaa ttgagaacaa ccccttcgcc
      841 aaaggctttc ggggcagtga tgacctggag ttacacagga tgtctcggat gcaaagtaaa
      901 gagtatcctg tggttcccag gagcacagtg aggcacaaag tcacctccaa ccacagcccc
      961 ttcagcagcg agacccgagc tctctccacc tcatccaatt tagggtccca gtaccagtgt
     1021 gagaatggtg tctctggccc ctcccaggac cttctgcccc cacctaaccc atacccactg
     1081 gcccaggagc acagccaaat ttaccactgt accaagagga aagatgagga atgttccagc
     1141 acggagcacc cctataagaa gccgtacatg gagacatccc ccagcgagga agacaccttc
     1201 tatcgctcgg gctaccccca gcagcagggc ctgagtacct cttacaggac agagtcggcc
     1261 cagcggcagg cctgcatgta tgccagctcc gctcccccca gcgagcccgt gcctagcctg
     1321 gaggacatca gctgtaacac atggcccagc atgccctcct atagcagctg taccgtcacc
     1381 accgtgcagc ccatggaccg tcttccctac cagcacttct ccgctcattt cacctcgggg
     1441 cccctggtcc ctcggttggc tggcatggcc aaccatggtt ctccccagct cggcgaaggg
     1501 atgtttcagc accagacctc agtggcccat cagcctgtgg tcaggcagtg cgggcctcag
     1561 actggccttc agtctccggg cggcctccag cccccagagt ttctctacac tcacggcgtg
     1621 cccaggaccc tgtcccccca tcagtatcac tcggtacacg gcgtcggcat ggtgccagag
     1681 tggagtgaga atagccactc gagatatcta gacccagctt tcttgtacaa agttggcatt
     1741 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1801 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     1861 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1921 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     1981 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2041 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2101 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2161 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2221 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2281 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2341 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2401 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2461 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2521 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2581 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2641 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2701 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     2761 gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagaccccg tagaaaagat
     2821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2941 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3361 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3421 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3481 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta gcatggatct
     3541 cggggacgtc taactactaa gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     3601 gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     3661 agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg
     3721 cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg
     3781 gatggccttt ttgcgtttct acaaactctt cctgttagtt agttacttaa gctcgggccc
//
