LOCUS       sequence-189195-       10959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10959)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-KanMx4
FEATURES             Location/Qualifiers
     source          1..10959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..59
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(97..119)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     219..238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     gene            427..1783
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        427..770
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             771..1580
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(838..857)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1586..1783
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        1858..2524
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2394..2417
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      2553..2562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2559..4277
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6672..6692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6720..6907
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6775..6799)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(7025..7044)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(7126..7394)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(7425..7445)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7425..7443)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7464..7480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7464..7480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7477..7499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7488..7504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7512..7518,7519..7536,7537..7542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7557..7578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7695..7712)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7866..8454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7946..7965)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8625..9416,9417..9485))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9248..9267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9486..9590)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9617..10959
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10580..10627)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
       61 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
      121 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
      181 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
      241 gagagtgcac cataccacct tttcaattca tcattttttt tttattcttt tttttgattt
      301 cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag
      361 gagcacagac ttagattggt atatatacgc aagcttgcct cgtccccgcc gggtcacccg
      421 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
      481 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
      541 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc
      601 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg
      661 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt
      721 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaagg
      781 aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      841 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtacggga
      901 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      961 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     1021 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag
     1081 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     1141 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     1201 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     1261 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
     1321 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     1381 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     1441 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     1501 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     1561 tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt
     1621 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     1681 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     1741 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     1801 acgccgcctc cagtgtcgaa tgatatcgaa ttcagtctac tagtcagttc gagtttatca
     1861 ttatcaatac tgccatttca aagaatacgt aaataattaa tagtagtgat tttcctaact
     1921 ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg
     1981 gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc
     2041 atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc
     2101 agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca
     2161 actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc
     2221 aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt
     2281 ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt
     2341 tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg
     2401 tattgattgt aattctgtaa atctatttct taaacttctt aaattctact tttatagtta
     2461 gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa
     2521 caaaagatct gatatggatc gaattagatc tcgccaccat ggacaagaag tattctatcg
     2581 gactggacat cgggactaat agcgtcgggt gggccgtcat cactgacgag tacaaggtgc
     2641 cctctaagaa gttcaaggtg ctcgggaaca ccgaccggca ttccatcaag aaaaatctga
     2701 tcggagctct cctctttgat tcaggggaga ccgctgaagc aacccgcctc aagcggactg
     2761 ctagacggcg gtacaccagg aggaagaacc ggatttgtta ccttcaagag atattctcca
     2821 acgaaatggc aaaggtcgac gacagcttct tccataggct ggaagaatca ttcctcgtgg
     2881 aagaggataa gaagcatgaa cggcatccca tcttcggtaa tatcgtcgac gaggtggcct
     2941 atcacgagaa atacccaacc atctaccatc ttcgcaaaaa gctggtggac tcaaccgaca
     3001 aggcagacct ccggcttatc tacctggccc tggcccacat gattaagttc agaggccact
     3061 tcctgatcga gggcgacctc aatcctgaca atagcgatgt ggataaactg ttcatccagc
     3121 tggtgcagac ttacaaccag ctctttgaag agaaccccat caatgcaagc ggagtcgatg
     3181 ccaaggccat tctgtcagcc cggctgtcaa agagccgcag acttgagaat cttatcgctc
     3241 agctgccggg tgaaaagaaa aatggactgt tcgggaacct gattgctctt tcacttgggc
     3301 tgactcccaa tttcaagtct aatttcgacc tggcagagga tgccaagctg caactgtcca
     3361 aggacaccta tgatgacgat ctcgacaacc tcctggccca gatcggtgac caatacgccg
     3421 accttttcct tgctgctaag aatctttctg acgccatcct gctgtctgac attctccgcg
     3481 tgaacactga aatcaccaag gcccctcttt cagcttcaat gattaagcgg tatgatgagc
     3541 accaccagga cctgaccctg cttaaggcac tcgtccggca gcagcttccg gagaagtaca
     3601 aggaaatctt ctttgaccag tcaaagaatg gatacgccgg ctacatcgac ggaggtgcct
     3661 cccaagagga attttataag tttatcaaac ctatccttga gaagatggac ggcaccgaag
     3721 agctcctcgt gaaactgaat cgggaggatc tgctgcggaa gcagcgcact ttcgacaatg
     3781 ggagcattcc ccaccagatc catcttgggg agcttcacgc catccttcgg cgccaagagg
     3841 acttctaccc ctttcttaag gacaacaggg agaagattga gaaaattctc actttccgca
     3901 tcccctacta cgtgggaccc ctcgccagag gaaatagccg gtttgcttgg atgaccagaa
     3961 agtcagaaga aactatcact ccctggaact tcgaagaggt ggtggacaag ggagccagcg
     4021 ctcagtcatt catcgaacgg atgactaact tcgataagaa cctccccaat gagaaggtcc
     4081 tgccgaaaca ttccctgctc tacgagtact ttaccgtgta caacgagctg accaaggtga
     4141 aatatgtcac cgaagggatg aggaagcccg cattcctgtc aggcgaacaa aagaaggcaa
     4201 ttgtggacct tctgttcaag accaatagaa aggtgaccgt gaagcagctg aaggaggact
     4261 atttcaagaa aattgaatgc ttcgactctg tggagattag cggggtcgaa gatcggttca
     4321 acgcaagcct gggtacctac catgatctgc ttaagatcat caaggacaag gattttctgg
     4381 acaatgagga gaaagaggac atccttgagg acattgtcct gactctcact ctgttcgagg
     4441 accgggaaat gatcgaggag aggcttaaga cctacgccca tctgttcgac gataaagtga
     4501 tgaagcaact taaacggaga agatataccg gatggggacg ccttagccgc aaactcatca
     4561 acggaatccg ggacaaacag agcggaaaga ccattcttga tttccttaag agcgacggat
     4621 tcgctaatcg caacttcatg caacttatcc atgatgattc cctgaccttt aaggaggaca
     4681 tccagaaggc ccaagtgtct ggacaaggtg actcactgca cgagcatatc gcaaatctgg
     4741 ctggttcacc cgctattaag aagggtattc tccagaccgt gaaagtcgtg gacgagctgg
     4801 tcaaggtgat gggtcgccat aaaccagaga acattgtcat cgagatggcc agggaaaacc
     4861 agactaccca gaagggacag aagaacagca gggagcggat gaaaagaatt gaggaaggga
     4921 ttaaggagct cgggtcacag atccttaaag agcacccggt ggaaaacacc cagcttcaga
     4981 atgagaagct ctatctgtac taccttcaaa atggacgcga tatgtatgtg gaccaagagc
     5041 ttgatatcaa caggctctca gactacgacg tggaccacat cgtccctcag agcttcctca
     5101 aagacgactc aattgacaat aaggtgctga ctcgctcaga caagaaccgg ggaaagtcag
     5161 ataacgtgcc ctcagaggaa gtcgtgaaaa agatgaagaa ctattggcgc cagcttctga
     5221 acgcaaagct aatcactcag cggaagttcg acaatctcac taaggctgag aggggcggac
     5281 tgagcgaact ggacaaagca ggattcatta aacggcaact tgtggagact cggcagatta
     5341 ctaaacatgt agcccaaatc cttgactcac gcatgaatac caagtacgac gaaaacgaca
     5401 aacttatccg cgaggtgaag gtgattaccc tgaagtccaa gctggtcagc gatttcagaa
     5461 aggactttca attctacaaa gtgcgggaga tcaataacta tcatcatgct catgacgcat
     5521 atctgaatgc cgtggtggga accgccctaa tcaagaagta cccaaagctg gaaagcgagt
     5581 tcgtgtacgg agactacaag gtctacgacg tgcgcaagat gattgccaaa tctgagcagg
     5641 agatcggaaa ggccaccgca aagtacttct tctacagcaa catcatgaat ttcttcaaga
     5701 ccgaaatcac ccttgcaaac ggtgagatcc ggaagaggcc gctcatcgag actaatgggg
     5761 agactggcga aatcgtgtgg gacaagggca gagatttcgc taccgtgcgc aaagtgcttt
     5821 ctatgcctca agtgaacatc gtgaagaaaa ccgaggtgca aaccggaggc ttttctaagg
     5881 aatcaatcct ccccaagcgc aactccgaca agctcattgc aaggaagaag gattgggacc
     5941 ctaagaagta cggcggattc gattcaccaa ctgtggctta ttctgtcctg gtcgtggcta
     6001 aggtggaaaa aggaaagtct aagaagctca agagcgtgaa ggaactgctg ggtatcacca
     6061 ttatggagcg cagctccttc gagaagaacc caattgactt tctcgaagcc aaaggttaca
     6121 aggaagtcaa gaaggacctt atcatcaagc tcccaaagta tagcctgttc gaactggaga
     6181 atgggcggaa gcggatgctc gcctccgctg gcgaacttca gaagggtaat gagctggctc
     6241 tcccctccaa gtacgtgaat ttcctctacc ttgcaagcca ttacgagaag ctgaagggga
     6301 gccccgagga caacgagcaa aagcaactgt ttgtggagca gcataagcat tatctggacg
     6361 agatcattga gcagatttcc gagttttcta aacgcgtcat tctcgctgat gccaacctcg
     6421 ataaagtcct tagcgcatac aataagcaca gagacaaacc aattcgggag caggctgaga
     6481 atatcatcca cctgttcacc ctcaccaatc ttggtgcccc tgccgcattc aagtacttcg
     6541 acaccaccat cgaccggaaa cgctatacct ccaccaaaga agtgctggac gccaccctca
     6601 tccaccagag catcaccgga ctttacgaaa ctcggattga cctctcacag ctcggagggg
     6661 atgagggagc tcccaagaaa aagcgcaagg taggtagttc cggatcctag actctcgagg
     6721 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     6781 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     6841 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
     6901 ctaccggcat gccgagcaaa tgcctgcaaa tcgctcccca tttctctaga gcggccgtgg
     6961 tatcgtttag attggcaatt acagtgtctt agctcacatg cttataacta attacatgac
     7021 tcgaagacat aaaaaacaaa aaaagcacca ccgactcggt gccacttttt caagttgata
     7081 acggactagc cttattttaa cttgctattt aaatgcatcg attctgatca tttatctttc
     7141 actgcggaga agtttcgaac gccgaaacat gcgcaccaac tttcacttct acagcgtttg
     7201 accaaaatct tttgaacaga acattgtagg gtgtgaaaaa atgcgcacct ttaccgctag
     7261 cccaagaggg cactacaaaa tctagagttg tacttcaaac gtacatgtaa tcaccttgta
     7321 tatactcgaa agaaaacatc aagtttctgt ataaatatga gtgaaagcat aatcatacat
     7381 tatcttttca aagattgtcc gcggtggagc tccagctttt gttcccttta gtgagggtta
     7441 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7501 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7561 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7621 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7681 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7741 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7801 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7861 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7921 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7981 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8041 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8101 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8161 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8221 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8281 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8341 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8401 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8461 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8521 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8581 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8641 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8701 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8761 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8821 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8881 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8941 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9001 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9061 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9121 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9181 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9241 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9301 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9361 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9421 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9481 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9541 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9601 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     9661 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     9721 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     9781 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     9841 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     9901 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     9961 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
    10021 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
    10081 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
    10141 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
    10201 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
    10261 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
    10321 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
    10381 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
    10441 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
    10501 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
    10561 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
    10621 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
    10681 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
    10741 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
    10801 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
    10861 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
    10921 ctcatccttc aatgctatca tttcctttga tattggatc
//
