LOCUS       sequence-18921-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Naviaux RK, Costanzi E, Haas M, Verma IM
  TITLE     The pCL vector system: rapid production of helper-free, high-titer, 
            recombinant retroviruses.
  JOURNAL   J Virol. 1996 Aug . 70(8):5701-5.
  PUBMED    8764092
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1996 Aug . 70(8):5701-5."
COMMENT     Sequence Label: pCLXSN
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(65..85)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        70..399
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      250..385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(513..532)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaattg cggccgcaat tcgttaactc gaggatccgg
       61 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
      121 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      241 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      301 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      361 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgggct gcaggtcgag
      421 gcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
      481 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      541 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
      601 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
      661 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
      721 gaagggactg gctgctattg ggcga
//
