LOCUS       sequence-189228-        9640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of nuclear LacZ driven by Gfa2 promoter with 3 additional
            copies of the gfa28 ABD segment; has about 100 times higher activity
            than pGfa2-nLac in transfected cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   de Leeuw B, Su M, ter Horst M, Iwata S, Rodijk M, Hoeben RC, Messing
            A, Smitt PS, Brenner M
  TITLE     Increased glia-specific transgene expression with glial fibrillary 
            acidic protein promoters containing multiple enhancer elements.
  JOURNAL   J Neurosci Res. 2006 Apr;83(5):744-53.
  PUBMED    16496373
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Res."; date: "2006-04"; volume: "83(5)"; pages: "744-53"
COMMENT     Sequence Label: pGfa2(ABDx3)-nLac
FEATURES             Location/Qualifiers
     source          1..9640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3572..3592
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3611..6658
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(7244..7261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7244..7260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7469..7488)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7588..7610
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7648..7666)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7734..7838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7839..7907,7908..8699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8870..9458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9359..9378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9612..9629
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
      241 ggtaccagat ctgagctccc acctccctct ctgtgctggg actcacagag ggagacctca
      301 ggaggcagtc tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg
      361 cgcacaactg taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga
      421 agttctagac cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa
      481 tcagccacgt gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg
      541 gatcacttaa ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca
      601 gcctgggcaa cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa
      661 catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc
      721 tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat
      781 tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca
      841 gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa
      901 tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg
      961 catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg
     1021 gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca
     1081 ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag
     1141 gacacaaatg ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc
     1201 gtagatggac ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc
     1261 ccagtggctc cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg
     1321 ctgatgtgtg ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga
     1381 aacggggcgc tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg
     1441 tggagctgtc aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg
     1501 tgacctggag ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc
     1561 ctcttgccct atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag
     1621 ggctggtgtc caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc
     1681 aggaatgtgg gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc
     1741 tagtaggaaa tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc
     1801 agcaccttgc aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt
     1861 tggaaagcag gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt
     1921 tgtgagacaa ggtctctctc tgttgcccag gctggagtgg cgcaaacaca gctcactgca
     1981 gcctcaacct actgggctca agcaatcctc cagcctcagc ctcccaaagt gctgggatta
     2041 caagcatgag ccaccccact cagccctttc cttccttttt aattgatgca taataattgt
     2101 aagtattcat catggtccaa ccaacccttt cttgacccac cttcctagag agagggtcct
     2161 cttgcttcag cggtcagggc cccagaccca tggtctggct ccaggtacca cctgcctcat
     2221 gcaggagttg gcgtgcccag gaagctctgc ctctgggcac agtgacctca gtggggtgag
     2281 gggagctctc cccatagctg ggctgcggcc caaccccacc ccctcaggct atgccagggg
     2341 gtgttgccag gggcacccga tctaacatat cctggtgtgg agtaggggac gctgctctga
     2401 cagaggctcg ggggcctgag ctggctctgt gagctgggga ggaggcagac agccaggcct
     2461 tgtctgcaag cagacctggc agcattgggc tggccgcccc ccagggcctc ctcttcatgc
     2521 ccagtgaatg actcaccttg gcacagacac aatgttcggg gtgggcacag tgcctgcttc
     2581 ccgccgcacc ccagcccccc tcaaatgcct tccgagaagc ccattgagca gggagctctc
     2641 cccatagctg ggctgcggcc caaccccacc ccctcaggct atgccagggg gtgttgccag
     2701 gggcacccga tctaacatat cctggtgtgg agtaggggac gctgctctga cagaggctcg
     2761 ggggcctgag ctggctctgt gagctgggga ggaggcagac agccaggcct tgtctgcaag
     2821 cagacctggc agcattgggc tggccgcccc ccagggcctc ctcttcatgc ccagtgaatg
     2881 actcaccttg gcacagacac aatgttcggg gtgggcacag tgcctgcttc ccgccgcacc
     2941 ccagcccccc tcaaatgcct tccgagaagc ccattgagca gggagctctc cccatagctg
     3001 ggctgcggcc caaccccacc ccctcaggct atgccagggg gtgttgccag gggcacccga
     3061 tctaacatat cctggtgtgg agtaggggac gctgctctga cagaggctcg ggggcctgag
     3121 ctggctctgt gagctgggga ggaggcagac agccaggcct tgtctgcaag cagacctggc
     3181 agcattgggc tggccgcccc ccagggcctc ctcttcatgc ccagtgaatg actcaccttg
     3241 gcacagacac aatgttcggg gtgggcacag tgcctgcttc ccgccgcacc ccagcccccc
     3301 tcaaatgcct tccgagaagc ccattgagca gggagctctc cccatagctg ggctgcggcc
     3361 caaccccacc ccctcaggct atgccagggg gtgttgccag gggcacccgg gcatcgccag
     3421 tctagcccac tccttcataa agccctcgca tcccaggagc gagcagagcc agagcaggtt
     3481 ggagaggaga cgcatcacct ccgctgctcg cggggatcct ctagagtcga cggatccggg
     3541 gaattcccca gtctcaggat ccaccatggg gcccaagaag aaacgcaaag tggggagcat
     3601 gggggatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3661 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3721 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
     3781 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
     3841 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
     3901 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
     3961 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
     4021 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
     4081 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
     4141 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
     4201 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
     4261 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
     4321 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
     4381 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
     4441 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
     4501 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
     4561 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
     4621 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
     4681 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
     4741 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
     4801 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
     4861 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
     4921 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg
     4981 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
     5041 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
     5101 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
     5161 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
     5221 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
     5281 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
     5341 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg
     5401 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca
     5461 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
     5521 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag
     5581 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact
     5641 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc
     5701 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa
     5761 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
     5821 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct
     5881 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac
     5941 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
     6001 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
     6061 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca
     6121 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
     6181 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa
     6241 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa
     6301 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
     6361 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
     6421 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca
     6481 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
     6541 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc
     6601 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata
     6661 ataaccgggc aggggggatc cgcagatccc ggccagatac cgatgctgcc gcagcaaaag
     6721 caggagcaga tgccgccgtc gcaggcgaag atgtcgcaga cggaggaggc gatgctgccg
     6781 gcggaggagg cgaagtaagt agagggctgg gctgggctgt ggggggtgtg gggtgcggga
     6841 ctgggcagtc tgggagtccc tctcaccact tttcttacct ttctaggatg ctgccgtcgc
     6901 cgccgctcat acaccataag gtgtaaaaaa tactagatgc acagaatagc aagtccatca
     6961 aaactcctgc gtgagaattt taccagactt caagagcatc tcgccacatc ttgaaaaatg
     7021 ccaccgtccg atgaaaaaca ggagcctgct aaggaacaat gccacctgtc aataaatgtt
     7081 gaaaactcat cccattcctg cctcttggtc cttgggcttg gggaggggtg cgcggatgtg
     7141 gttagggaac atgactggtc aaatgggaag ggcttcaaaa gaattcccaa tattgactac
     7201 caagccacct gtacagatct gcctgcaggc atgcaagctt ggcactggcc gtcgttttac
     7261 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7321 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7381 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7441 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7501 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7561 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7621 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7681 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9481 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9541 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9601 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
