LOCUS       sequence-189262-        7666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions / Actin, Excitation: 683, Emission: 
            704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..7666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3310..4269
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    4394..4515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4431..4450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4485..4504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4522..4977)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4659..4680)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4871..4890
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5004..5108
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5110..5467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5110..5301
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5133..5153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5318..5453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5502..6296
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5502..6296
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5556..5575)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6166..6185
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6484..6503)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6528..6575
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6904..7492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7393..7412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcgc accatcatgg accattatga ttctcagcaa
      601 accaacgatt acatgcagcc agaagaggac tgggaccggg acctgctcct ggacccggcc
      661 tgggagaagc agcagagaaa gacattcacg gcatggtgta actcccacct ccggaaggcg
      721 gggacacaga tcgagaacat cgaagaggac ttccgggatg gcctgaagct catgctgctg
      781 ctggaggtca tctcaggtga acgcttggcc aagccagagc gaggcaagat gagagtgcac
      841 aagatctcca acgtcaacaa ggccctggat ttcatagcca gcaaaggcgt caaactggtg
      901 tccatcggag ccgaagaaat cgtggatggg aatgtgaaga tgaccctggg catgatctgg
      961 accatcatcc tgcgctttgc catccaggac atctccgtgg aagagacttc agccaaggaa
     1021 gggctgctcc tgtggtgtca gagaaagaca gccccttaca aaaatgtcaa catccagaac
     1081 ttccacataa gctggaagga tggcctcggc ttctgtgctt tgatccaccg acaccggccc
     1141 gagctgattg actacgggaa gctgcggaag gatgatccac tcacaaatct gaatacggct
     1201 tttgacgtgg cagagaagta cctggacatc cccaagatgc tggatgccga agacatcgtt
     1261 ggaactgccc gaccggatga gaaagccatc atgacttacg tgtctagctt ctaccacgcc
     1321 ttctctggag cccagaaggc ggagacagca gccaatcgca tctgcaaggt gttggccgtc
     1381 aaccaggaga acgagcagct tatggaagac tacgagaagc tggccagtga tctgttggag
     1441 tggatccgcc gcacaatccc gtggctggag aaccgggtgc ccgagaacac catgcatgcc
     1501 atgcaacaga agctggagga cttccgggac taccggcgcc tgcacaagcc gcccaaggtg
     1561 caggagaagt gccagctgga gatcaacttc aacacgctgc agaccaagct gcggctcagc
     1621 aaccggcctg ccttcatgcc ctctgagggc aggatggtct cggacatcaa caatgcctgg
     1681 ggctgcctgg agcaggtgga gaagggctat gaggagtggt tgctgaatga gatccggagg
     1741 ctggagcgac tggaccacct ggcagagaag ttccggcaga aggcctccat ccacgaggcc
     1801 tggactgacg gcaaagaggc catgctgcga cagaaggact atgagaccgc caccctctcg
     1861 gagatcaagg ccttgctcaa gaagcatgag gccttcgaga gtgacctggc tgcccaccag
     1921 gaccgtgtgg agcagattgc cgccatcgca caggagctca atgagctgga ctattatgac
     1981 tcacccagtg tcaacgcccg ttgccaaaag atctgtgacc agtgggacaa tctgggggcc
     2041 ctaactcaga agcgaaggga agctctggag cggaccgaga aactgctgga gaccattgac
     2101 cagctgtact tggagtatgc caagcgggct gcacccttca acaactggat ggagggggcc
     2161 atggaggacc tgcaggacac cttcattgtg cacaccattg aggagatcca gggactgacc
     2221 acagcccatg agcagttcaa ggccaccctc cctgatgccg acaaggagcg cctggccatc
     2281 ctgggcatcc acaatgaggt gtccaagatt gtccagacct accacgtcaa tatggcgggc
     2341 accaacccct acacaaccat cacgcctcag gagatcaatg gcaaatggga ccacgtgcgg
     2401 cagctggtgc ctcggaggga ccaagctctg acggaggagc atgcccgaca gcagcacaat
     2461 gagaggctac gcaagcagtt tggagcccag gccaatgtca tcgggccctg gatccagacc
     2521 aagatggagg agatcgggag gatctccatt gagatgcatg ggaccctgga ggaccagctc
     2581 agccacctgc ggcagtatga gaagagcatc gtcaactaca agccaaagat tgatcagctg
     2641 gagggcgacc accagctcat ccaggaggcg ctcatcttcg acaacaagca caccaactac
     2701 accatggagc acatccgtgt gggctgggag cagctgctca ccaccatcgc caggaccatc
     2761 aatgaggtag agaaccagat cctgacccgg gatgccaagg gcatcagcca ggagcagatg
     2821 aatgagttcc gggcctcctt caaccacttt gaccgggatc actccggcac actgggtccc
     2881 gaggagttca aagcctgcct catcagcttg ggttatgata ttggcaacga cccccaggga
     2941 gaagcagaat ttgcccgcat catgagcatt gtggacccca accgcctggg ggtagtgaca
     3001 ttccaggcct tcattgactt catgtcccgc gagacagccg acacagatac agcagaccaa
     3061 gtcatggctt ccttcaagat cctggctggg gacaagaact acattaccat ggacgagctg
     3121 cgccgcgagc tgccacccga ccaggctgag tactgcatcg cgcggatggc cccctacacc
     3181 ggccccgact ccgtgccagg tgctctggac tacatgtcct tctccacggc gctgtacggc
     3241 gagagtgacc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     3301 gtcgccacca tgtcggtacc gctgactacc tcagcattcg gccacgcgtt tctggctaac
     3361 tgtgaacgcg agcagatcca cctggcgggc tccattcagc cgcacggtat cctgctggct
     3421 gtgaaagagc cggacaacgt ggtgatccag gcttctatta acgctgcgga gttcctgaac
     3481 accaactctg ttgttggccg tccgctgcgt gacctgggcg gcgatctgcc tttgcagatc
     3541 ctgccgcacc tgaacggccc gctgcacctg gctccgatga ccctgcgttg taccgtgggt
     3601 tctccgccgc gtcgtgtgga ctgtaccatt catcgtccgt ctaacggcgg cctgatcgta
     3661 gaactggaac cagcaaccaa gaccactaac attgcgccgg ctctggacgg tgcgtttcat
     3721 cgtatcactt cttcatcctc cctgatgggc ctgtgtgacg aaaccgcgac tattatccgt
     3781 gagattactg gctacgaccg tgtgatggta gtacgtttcg atgaagaggg taatggcgaa
     3841 attctgtccg aacgtcgtcg tgcggacctg gaagcgttcc tgggtaaccg ctacccggcg
     3901 tctactattc cgcagatcgc tcgtcgcctg tacgaacata accgtgttcg cctgctggta
     3961 gatgtgaact atactccggt tccgctacag ccgcgcatca gcccgctgaa cggtcgtgat
     4021 ctggatatgt ccctgtcttg cctgcgctct atgtccccga tccaccagaa atacatgcag
     4081 gacatgggcg ttggcgcgac cctggtttgc tctctgatgg tgtctggtcg tctgtggggt
     4141 ctgatcgctt gccaccacta cgaaccgcgc ttcgttccgt tccacattcg cgctgctggc
     4201 gaagcgctgg cggaaacttg tgcgatccgc atcgcgacgc tggagagctt tgcacagtct
     4261 cagtccaaat gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     4321 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4381 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4501 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4561 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4621 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4681 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4741 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4801 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4861 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4921 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     4981 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5041 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5101 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     5161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5221 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5281 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5341 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5401 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5461 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5521 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5581 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5641 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     5701 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5761 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5821 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5881 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5941 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6001 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     6061 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6121 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6181 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6241 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6301 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     6361 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6421 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     6481 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     6541 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     6601 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     6661 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     6721 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     6781 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7561 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
     7621 aatcaattac ggggtcatta gttcatagcc catatatgga gttccg
//
