LOCUS       sequence-189267-       10956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-MyosinIIC-N-18
FEATURES             Location/Qualifiers
     source          1..10956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..406
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        49..240
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(72..92)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      257..392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             441..1235
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             441..1235
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(495..514)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1105..1124
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1423..1442)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1467..1514
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1843..2431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2332..2351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2606..2909
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2910..3113
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3064..3084
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3145..3154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9205..10164
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    10289..10410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10326..10345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10380..10399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10417..10872)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10554..10575)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10766..10785
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(10899..10956,1..47)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
       61 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
      121 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      181 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
      241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
      301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
      361 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
      421 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
      481 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      541 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      601 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
      661 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      721 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      781 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      841 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
      901 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
      961 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     1021 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     1081 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     1141 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     1201 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     1261 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     1321 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     1381 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     1441 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     1501 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     1561 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     1621 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     1681 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1741 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1981 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2461 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2521 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
     2581 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     2641 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2701 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2761 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2821 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2881 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2941 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     3001 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     3061 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     3121 tgaaccgtca gatccgctag caccgccacc atggctgcag tgaccatgtc cgtgtctggg
     3181 aggaaggtag cctccaggcc aggcccggtg cctgaggcag cccaatcgtt cctctacgcg
     3241 ccccggacgc caaatgtagg tggccctgga gggccacagg tggagtggac agcccggcgc
     3301 atggtgtggg tgccctcgga actgcatggg ttcgaggcag cagccctgcg ggatgaaggg
     3361 gaggaggagg cagaagtgga gctggcggag agtgggcgcc gcctgcggct gcccagggac
     3421 cagatccagc gcatgaaccc acccaagttc agcaaggcag aagatatggc tgagctcacc
     3481 tgcctcaacg aggcctcggt cctgcacaac ctgcgagaac gctactactc cgggctcatt
     3541 tatacctact ctggcctctt ctgtgtggtc attaacccat acaagcagct gcccatctac
     3601 acggaggcca ttgttgaaat gtaccggggc aagaagcgcc atgaggtgcc acctcacgtg
     3661 tatgctgtga cggagggcgc gtaccgcagc atgcttcagg atcgtgagga tcaatccatt
     3721 ctctgcacgg gagagtctgg cgctgggaag acggagaaca ccaagaaggt catccagtac
     3781 ctggcccatg tggcatcatc tccaaagggc aggaaggagc ctggtgtccc tgcctccgtc
     3841 agcaccatgt cttatgggga gctagagcgt cagcttcttc aagccaaccc catcctagag
     3901 gcctttggca atgccaagac agtgaagaac gacaactctt cccgatttgg caaattcatc
     3961 cgcatcaact ttgatattgc tggctacatc gtgggagcaa acatcgagac ctatctgttg
     4021 gagaagtccc gggccatcag acaggccaag gatgaatgca gcttccatat cttctaccag
     4081 ctgctagggg gcgctgggga gcagctaaaa gctgacctcc ttctggagcc ctgttcccat
     4141 tatcgcttcc tgaccaatgg gccctcatcg tccccgggcc aggagcgtga gttattccag
     4201 gagaccctgg agtccctgcg tgtgctgggc ctcctcccag aagagatcac tgccatgctg
     4261 cgcactgtct ctgctgtcct ccagtttggc aacattgtcc tgaagaaaga gcgcaatacg
     4321 gaccaagcca ccatgcctga caacacagct gcccagaagc tttgccgcct cttgggactc
     4381 ggagtgaccg acttctccag agcccttctc acaccccgca tcaaagtggg ccgagattat
     4441 gttcagaaag cacaaaccaa ggagcaggct gactttgcgc tggaggctct ggccaaagct
     4501 acctatgagc gcctgttccg ctggctggtt ctgcggctca accgtgccct ggacagaagc
     4561 ccgcggcagg gtgcctcctt cctgggcatc ctggacatcg cgggctttga gatcttccag
     4621 ctgaactcct tcgagcagct gtgcatcaac tacaccaacg agaagctaca gcagctattc
     4681 aaccacacca tgttcgtgct ggagcaggag gagtaccagc gagagggcat cccctggacc
     4741 ttcctagact tcgggttgga cctgcaacct tgcatcgacc tcattgagcg tccggccaac
     4801 cctccaggtc tcctggccct gctggacgag gagtgctggt tccccaaggc cacggacaag
     4861 tcttttgtgg agaaggtcgc ccaggagcag ggcagccacc ccaaattcca gcgccccagg
     4921 aacctgcgag atcaggccga cttcagcgtc ctgcactacg ccggcaaggt tgactacaaa
     4981 gccagtgagt ggctgatgaa gaacatggac ccactgaatg acaatgtggc cgccttgctt
     5041 caccagagca cggatcgtct cacagctgag atctggaagg atgtggaggg catcgtgggg
     5101 ctggagcaag taagcagcct tggagatggc ccaccgggag gccgcccccg ccgtggaatg
     5161 tttcggactg tggggcagct ctacaaagaa tccctgagcc gcctcatggc cacgctcagc
     5221 aacaccaacc ctagttttgt ccgctgcatc gttcccaatc atgagaagag ggctggaaag
     5281 ctggagccgc gcctggtgct ggaccaactc cgttgtaacg gggtcctcga gggtatacgc
     5341 atctgtcgcc aaggcttccc caaccgcatc ctcttccagg agttccgaca gcgctatgaa
     5401 atcctcaccc cgaacgctat tcccaagggc ttcatggacg gcaaacaggc ctgtgagaag
     5461 atgatccagg ccctggagct agaccccaac ctgtaccgtg ttggccaaag caagatcttc
     5521 ttccgggcag gggtcctggc ccagctggag gaggagcggg acctgaaagt caccgacatc
     5581 atagtgtctt tccaggcagc ggcacggggc tacctggccc gtagggcttt ccagagacgg
     5641 cagcagcagc agagtgctct gagggtgatg cagagaaact gtgctgccta cctcaagctc
     5701 aggaactggc agtggtggag gctgttcatc aaggtgaagc ccctgctgca ggtgacacgg
     5761 caggatgagg tgctgcaggc gcgcgcccag gagctgcaga aagttcagga gctgcagcag
     5821 cagagcgctc gtgaagtggg ggaactgcag ggtcgagtgg cacagctaga ggaggagcgc
     5881 acgcgcctgg ctgagcagct tcgagcagaa gccgagctct gctctgaggc cgaggagacg
     5941 cgggcgcgac tggctgcccg gaagcaggag ctggagctgg tggtgacaga gctggaggca
     6001 cgagtgggcg aggaagaaga gtgcagccgg cagctgcaga gtgagaagaa gaggctgcag
     6061 cagcatatcc aggagctaga gagccacctg gaagctgagg agggtgcccg gcagaagcta
     6121 cagctggaga aggtgaccac agaggccaag atgaagaaat ttgaggagga cctgctgctc
     6181 ctggaggacc agaattccaa gctgagcaag gagcggaggc tgctggagga gcggctggct
     6241 gagttctcct cacaggcagc agaagaggaa gagaaagtca aaagtctcaa caagctgagg
     6301 ctcaaatatg aagccacaat ctcagacatg gaagaccggc tgaagaagga ggagaaggga
     6361 cgccaggaac tagagaagct gaagcgacgg ctggacgggg agagctcaga gcttcaggag
     6421 cagatggtgg agcagaagca gagggcagag gaactgctcg cacagctggg ccgcaaggag
     6481 gatgagctgc aggccgccct gctcagggca gaggaagagg gtggtgcccg tgcccagttg
     6541 ctcaagtccc tgcgagaggc acaggctggc cttgctgagg ctcaggagga cctggaagct
     6601 gagcgggtag ccagggccaa ggcggagaag cagcgccggg acctgggcga ggagttggag
     6661 gccctacgtg gggagctcga ggacactctg gattccacca acgcccagca ggagctgcgg
     6721 tccaagaggg agcaggaggt gacagagctg aagaaagcat tggaagagga gtcccgtgcc
     6781 catgaggtgt ccatgcagga gctgagacag aggcatagcc aggcactggt ggagatggcc
     6841 gagcagttgg agcaagcccg gaggggcaaa ggtgtgtggg agaagactcg gctatccctg
     6901 gaggctgagg tgtccgagct gaaggccgag ctgagcagcc tgcagacctc gagacaggag
     6961 ggtgagcaga agaggcgccg cctggagtcc cagctacagg aggtccaggg ccgatccagt
     7021 gattcggagc gggctcggtc tgaggctgct gagaagctgc agagagccca ggcggaactt
     7081 gagagcgtgt ccacagccct gagtgaggcg gagtccaaag ccatcaggct gggcaaggag
     7141 ctgagcagtg cagagtccca gctgcatgac acccaggaac tgcttcagga ggagaccagg
     7201 gcaaagctgg ccttggggtc ccgtgtgcgt gccctagagg ccgaggcggc ggggcttcgg
     7261 gagcagatgg aagaggaggt ggttgccagg gaacgggctg gccgggagct gcagagcacg
     7321 caggcccagc tctctgaatg gcggcgccgc caggaagaag aggctgcggt gctggaggct
     7381 ggggaggagg ctcggcgccg tgcagcccgg gaggcagaga ccctgaccca gcgcctggca
     7441 gaaaagactg aggctgtaga acgactggag cgagcccggc gccgactgca gcaggagttg
     7501 gacgatgcca ctgtggatct ggggcagcag aagcagctcc tgagcacact ggagaagaag
     7561 cagcggaaat ttgaccagct cctggcagag gagaaggctg cagttctacg ggctgtggaa
     7621 gaccgtgaac ggatagaggc cgaaggccgg gagcgagagg cccgggccct gtcgctgacc
     7681 cgggccctgg aagaggagca ggaggcccgg gaggagctgg agaggcagaa ccgtgctctg
     7741 agggctgagc tggaagcact gctgagcagc aaggatgacg tgggcaagaa cgtgcacgag
     7801 ctggagcgag cccgtaaggc ggctgaacag gcagccagtg acctgcggac acaggtgaca
     7861 gaattggagg atgagctgac agccgcagag gatgccaagc tgcgcctgga ggtgactgtg
     7921 caggctctga aggctcaaca tgaacgcgac ctgcagggcc gcgatgatgc cggtgaggag
     7981 aggcggaggc agctggccaa gcagctaaga gacgcagagg tagagcgcga tgaggaacgg
     8041 aagcagaggg cactggctat ggctgcccgc aagaagctgg agctggaact ggaggagttg
     8101 aaggcgcaga catctgctgc tgggcagggc aaggaagagg cagtgaagca gctgaagaag
     8161 atgcaggtcc agatgaagga gctgtggcgg gaggtagagg agacgcgtag ctcccgcgac
     8221 gagatgttta ccctgagcag ggaaaatgag aagaagctca aggggctgga agctgaggtg
     8281 ctgcgtctgc aagaggaact tgctgcctca gaccgagccc ggaggcaggc ccagcaagac
     8341 agagacgaga tggcagagga ggtggccagt ggcaatctta gcaaggcagc caccctggag
     8401 gaaaaacggc agctggaggg gcgactgagc cagttggaag aggagctgga ggaagaacag
     8461 aacaactcgg agctgctcaa ggaccattac cgaaagctag tgctacaggt cgagtccctc
     8521 accacagaac tgtctgccga acgaagtttc tcagccaagg ccgagagtgg acggcagcag
     8581 ctggagcggc agatccagga actgcgggcc cgcttgggtg aagaggatgc tggagcccga
     8641 gccaggcaga aaatgctgat cgctgctctg gagtctaaac tggcccaggc agaggagcag
     8701 ctggagcagg agagcaggga gcgcatcctc tctggcaagc tggtacgcag agctgagaag
     8761 cggctgaagg aggtagttct tcaggtggat gaagagcgca gggtggctga ccaggtccgg
     8821 gaccagctgg agaaaagcaa cctccggctg aagcagctca agaggcagct ggaggaggca
     8881 gaggaggagg catctcgggc acaggctggt cggaggcggc tgcagcggga gctggaggac
     8941 gtcactgagt ctgcagaatc catgaaccgg gaggtgacca cgctgaggaa caggctccgg
     9001 cgtggcccac ttacattcac cacacggact gtgcgccagg tgttccggct ggaagagggc
     9061 gtggcttctg acgaggaaga ggctgaagga gctgaacctg gctctgcacc aggccaggag
     9121 ccggaggctc cgccccctgc cacaccccaa agcagcggtg gagcaagcgc agccagtggt
     9181 agcgcggatc caccggtcgc caccatgtcg gtaccgctga ctacctcagc attcggccac
     9241 gcgtttctgg ctaactgtga acgcgagcag atccacctgg cgggctccat tcagccgcac
     9301 ggtatcctgc tggctgtgaa agagccggac aacgtggtga tccaggcttc tattaacgct
     9361 gcggagttcc tgaacaccaa ctctgttgtt ggccgtccgc tgcgtgacct gggcggcgat
     9421 ctgcctttgc agatcctgcc gcacctgaac ggcccgctgc acctggctcc gatgaccctg
     9481 cgttgtaccg tgggttctcc gccgcgtcgt gtggactgta ccattcatcg tccgtctaac
     9541 ggcggcctga tcgtagaact ggaaccagca accaagacca ctaacattgc gccggctctg
     9601 gacggtgcgt ttcatcgtat cacttcttca tcctccctga tgggcctgtg tgacgaaacc
     9661 gcgactatta tccgtgagat tactggctac gaccgtgtga tggtagtacg tttcgatgaa
     9721 gagggtaatg gcgaaattct gtccgaacgt cgtcgtgcgg acctggaagc gttcctgggt
     9781 aaccgctacc cggcgtctac tattccgcag atcgctcgtc gcctgtacga acataaccgt
     9841 gttcgcctgc tggtagatgt gaactatact ccggttccgc tacagccgcg catcagcccg
     9901 ctgaacggtc gtgatctgga tatgtccctg tcttgcctgc gctctatgtc cccgatccac
     9961 cagaaataca tgcaggacat gggcgttggc gcgaccctgg tttgctctct gatggtgtct
    10021 ggtcgtctgt ggggtctgat cgcttgccac cactacgaac cgcgcttcgt tccgttccac
    10081 attcgcgctg ctggcgaagc gctggcggaa acttgtgcga tccgcatcgc gacgctggag
    10141 agctttgcac agtctcagtc caaatgagcg gccgcgactc tagatcataa tcagccatac
    10201 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10261 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10321 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10381 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
    10441 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    10501 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    10561 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    10621 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    10681 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    10741 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    10801 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    10861 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10921 ttttctaaat acattcaaat atgtatccgc tcatga
//
