LOCUS       sequence-18928-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     pBOB plasmids
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBOB-CAG-iCRE-SD
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    171..204
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             710..721
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
       61 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
      121 gctcccaggc tcagatctgg tctaaccaga gagacccagt acagtccggc ataacttcgt
      181 ataatgtatg ctatacgaag ttatgccgga tgcagctctc gggccatgtg atgaaatgct
      241 aggcggctgt caaacctcca ctctaatact tctctctccg ggtcatccat cccatgcagg
      301 ctcacagggt gtaacaagcg ggtgttctct ccttcattgg cttcttctac cttctcttgc
      361 tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgat ccctggccct
      421 ggtgtgtagt tctgccaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg
      481 atatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag
      541 tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtaccga gctcgaattc
      601 caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
      661 gaagangccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
      721 aanggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
      781 atggaaagga cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc aacagccg
//
