LOCUS       sequence-189287-       11849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged Arabidopsis SEIPIN1 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC43-SEIPIN1
FEATURES             Location/Qualifiers
     source          1..11849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..46
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     38..55
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     39..55
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        481..825
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(893..921)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1516..1536
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2746..2998
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3015..3031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3015..3031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3028..3050)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3039..3055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3063..3069,3070..3087,3088..3093))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3108..3129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3320..3996
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4063..5088
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5129..5303
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5381..5405
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5830..6624
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6711..7299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7200..7219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7453..7470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7485..7625
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7551..7570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7711..7733
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7969..8163
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8229..9302)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9731..10360)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11661..11685
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
       61 gcttgcatgc ctgcaggtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
      121 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
      181 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
      241 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
      301 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      361 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgataaca tggtggagca
      421 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
      481 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
      541 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      601 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      661 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      721 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      781 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga ctctagagga
      841 tccccgggta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc
      901 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      961 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     1021 acctgttcca tggccaaccc tggtcaccac cctgacctac ggcgtgcagt gcttctcccg
     1081 ttaccctgat catatgaagc ggcacgactt cttcaagagc gccatgcctg agggatacgt
     1141 gcaggagagg accatcttct tcaaggacga cgggaactac aagacacgtg ctgaagtcaa
     1201 gtttgaggga gacaccctcg tcaacaggat cgagcttaag ggaatcgatt tcaaggagga
     1261 cggaaacatc ctcggccaca agttggaata caactacaac tcccacaacg tatacatcat
     1321 ggccgacaag caaaagaacg gcatcaaagc caacttcaag acccgccaca acatcgaaga
     1381 cggcggcgtg caactcgctg atcattatca acaaaatact ccaattggcg atggccctgt
     1441 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     1501 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1561 ggatgaacta tacaaaggcg cgccaatgag aatcctccaa aacaaaacaa tgaaagaaca
     1621 agacaaccaa ctcaaaatcc ctgaaccatt acgagctgac tggttcatgg ttctagtcac
     1681 catccaagct gatctaatct acaacgcact cgtggtatta tcctcacctt tctttctcct
     1741 ataccgctct taccgccgcg cagtcgttac cgtctccgca gcagaaaaag cggttaaacg
     1801 tgctccagcg cagatagctg gtggtgcggg gcgggttgtt aggaggacat ggtttggtat
     1861 attgggtgca tgccacgtgt caatggtaat ggtgttggct ttgattttgg cggttgttat
     1921 tggtgttggg attgtgagtt tgtatgtaga aaagcctgtg gttgtgagag acagattgtt
     1981 ctttgattac actgaagaaa accccagtgc ggtttttagt tttgataaga agaagagatc
     2041 gtttagtgtt cctgttggtc atagtgttca tgtctcttta gtgctttgga tgcctgaatc
     2101 tgaaattaat cgaagaattg gagtatttca gttgaaagta gagcttttat cgttgaaagg
     2161 cgagacaatc gcgagatcga gccaaccttg tatgttacgg ttcagaagca aacctataag
     2221 acttgcacga acatttgtga tgagtgttcc acttatagcc gggatagcaa acgaagcaca
     2281 aacaatgaga atcgatgcat taaagcacca agagaaaatg ccaaggacaa aagccgtgag
     2341 agcgactctg attccgcgtg cacaaactcg gacattgcct cagttatatg aagcagagat
     2401 cgttataaac tcaaaaccgc cctggattaa acgaatggct tataactgga aatggactct
     2461 ttgcgtatgg acttcaatgt acctttacgt cgcgattttg accgcacttc tttggtgttt
     2521 tagaccggtt ttgttccctt acacttcctc aagaaccatt tcggagagcg aaaacttaga
     2581 gatcgaagta gttgaagagg aacaagaaca agtcatggaa agacgacgga gggaaagaag
     2641 aaaccagcct cgtcgaagaa attttgctac tacacagaaa agttatacat agttaattaa
     2701 ctagttctag agcggccgcc accgcggtgg agctcgaatt tccccgatcg ttcaaacatt
     2761 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2821 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2881 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     2941 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3001 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3061 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
     3241 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3301 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3361 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     3421 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     3481 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3541 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
     3601 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     3661 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     3721 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     3781 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     3841 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     3901 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     3961 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
     4021 ctatctctct cgagctttcg cagatccggg gggcaatgag atatgaaaaa gcctgaactc
     4081 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     4141 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     4201 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     4261 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt tagcgagagc
     4321 ctgacctatt gcatctcccg ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc
     4381 gaactgcccg ctgttctaca accggtcgcg gaggctatgg atgcgatcgc tgcggccgat
     4441 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     4501 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     4561 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     4621 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     4681 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     4741 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     4801 cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg ggcgtatatg
     4861 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     4921 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     4981 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     5041 gatagtggaa accgacgccc cagcactcgt ccgagggcaa agaaatagag tagatgccga
     5101 ccgggatctg tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     5161 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     5221 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     5281 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
     5341 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     5401 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     5461 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     5521 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     5581 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     5641 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     5701 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     5761 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     5821 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     5881 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     5941 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     6001 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     6061 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     6121 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     6181 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     6241 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     6301 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     6361 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     6421 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     6481 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     6541 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     6601 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
     6661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6841 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7321 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     7501 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     7561 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     7621 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7681 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7741 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     7801 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     7861 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     7921 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     7981 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     8041 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     8101 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     8161 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     8221 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     8281 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     8341 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     8401 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     8461 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     8521 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     8581 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     8641 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     8701 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     8761 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     8821 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     8881 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     8941 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     9001 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     9061 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     9121 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     9181 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     9241 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     9301 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
     9361 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     9421 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     9481 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     9541 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     9601 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     9661 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     9721 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     9781 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     9841 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     9901 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     9961 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    10021 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    10081 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    10141 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    10201 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    10261 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    10321 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    10381 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    10441 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    10501 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    10561 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    10621 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    10681 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    10741 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    10801 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    10861 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    10921 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    10981 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    11041 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    11101 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    11161 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    11221 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    11281 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    11341 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    11401 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    11461 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    11521 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    11581 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    11641 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
    11701 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
    11761 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11821 gcgaaagggg gatgtgctgc aaggcgatt
//
