LOCUS       sequence-189289-       12323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged Arabidopsis SEIPIN2 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC43-SEIPIN2
FEATURES             Location/Qualifiers
     source          1..12323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..411
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(479..507)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1102..1122
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2806..3058
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3075..3091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3075..3091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3088..3110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3099..3115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3123..3129,3130..3147,3148..3153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3168..3189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3380..4056
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4123..5148
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5189..5363
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5441..5465
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5890..6684
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6771..7359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7260..7279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7513..7530
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7545..7685
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7611..7630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7771..7793
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8029..8223
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8289..9362)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9791..10420)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11721..11745
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11933..11955
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11947..11964
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11948..11964
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
       61 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
      121 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
      181 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
      241 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      301 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      361 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct
      421 agaggatccc cgggtaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt
      481 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      541 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      601 aaaactacct gttccatggc caaccctggt caccaccctg acctacggcg tgcagtgctt
      661 ctcccgttac cctgatcata tgaagcggca cgacttcttc aagagcgcca tgcctgaggg
      721 atacgtgcag gagaggacca tcttcttcaa ggacgacggg aactacaaga cacgtgctga
      781 agtcaagttt gagggagaca ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa
      841 ggaggacgga aacatcctcg gccacaagtt ggaatacaac tacaactccc acaacgtata
      901 catcatggcc gacaagcaaa agaacggcat caaagccaac ttcaagaccc gccacaacat
      961 cgaagacggc ggcgtgcaac tcgctgatca ttatcaacaa aatactccaa ttggcgatgg
     1021 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
     1081 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
     1141 tggcatggat gaactataca aaggcgcgcc aatggactcc gagtccgagt ccgagtcaaa
     1201 ccctagcacc acggacgaat tcgatcgatt cctcgacgca cctgacgaat tttactacga
     1261 ttgcttaccg attcggtcca attcccatca accttcgtct ctcttacggc ggcgaaagtc
     1321 ggcacatcgc cgggatttaa tttcgtccga tattgaaacg gagccatctt cttcttccga
     1381 tggatttgat gtcggtgaga aatccagcta tgtggaaaag aacgctgagc ttagagggga
     1441 tattgataca agcgacgtaa ttgaatcaac taaggactca attgatctat cttcagaaaa
     1501 ggagaacgat ttggatgtaa ttagttcgtc tgggaatgat atggatgtaa ttgattcggg
     1561 tcggaaccgg gttgacccgt ttcaggaaga atccacggtg actaccgtga gcagcgatga
     1621 tcaaggagac gacgactacg ccggatcggt accacagttt cgggagccac ctaattccac
     1681 tgagtggtct ctccttgggt ttttggttgg gctagtgatc aaagcaatcg aatttcaagt
     1741 cagtttcatg acaagcttac tcacatttcc tccatggctt ctccgtaact gttttctctt
     1801 ctttttcgat ccctttagca cgattaggtt tggcagacgc tttttaatgg caagagtagc
     1861 aggaataagt gatatgatct ttggttatat gaatcctttt aggttaaaag atacaaaaca
     1921 aatgttgagt attgtgtgca agtttggttg gggtatgttt tgggctgttt atgtaggcat
     1981 tgtgttgttt ggcttgttgg tttcgtcgct tatgattggt ggttatgtga ttaaccgtat
     2041 agcggataaa ccatttgagg tcaaggagac cttgaatttt gattacacca agaatagtcc
     2101 agaagcatat gtgcccatat cctcgtgtgc tggtgttgaa tgtgagggaa gttgcaagga
     2161 aagtaatgaa atgtcgaaga tcaggggttt gcgagttatc cctcgagacc agaagttaga
     2221 tatcattctt tcaatgacat tacctgagtc tgcgtacaat aaaaatctcg gcatgtttca
     2281 ggttcgggtg gacttcttat ctgtggatgg tcaaacgatt gctagtataa ggcgtccgtg
     2341 catgttaaga ttcagaagtg agcctattcg tcttgttcag acattcttca aagtggttcc
     2401 gttagtgaca ggatatgtct ctgagatcca aaccttgagc ttgaagttga aaggctttgt
     2461 agaaaaggac attcccactg cttgcttaaa gataataatc gaacaacggg cagaatttcg
     2521 acccggagca ggtattccag agctgtacga tgcatcccta tcggttgaat ccggtcttcc
     2581 cttcttcaga aaaattatat ggaaatggcg gaagactttg ttcgtctgga tcagcatgag
     2641 tttgttcatt acggaattgc ttttcacgtt ggtttgttgt agacctctta tcatcccaag
     2701 aacacaacct agagacagat cgccttcaaa cccaactgga gtctggaggt agttaattaa
     2761 ctagttctag agcggccgcc accgcggtgg agctcgaatt tccccgatcg ttcaaacatt
     2821 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2881 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2941 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3001 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3061 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3121 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3181 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
     3301 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3361 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3421 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     3481 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     3541 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3601 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
     3661 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     3721 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     3781 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     3841 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     3901 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     3961 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     4021 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
     4081 ctatctctct cgagctttcg cagatccggg gggcaatgag atatgaaaaa gcctgaactc
     4141 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     4201 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     4261 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     4321 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt tagcgagagc
     4381 ctgacctatt gcatctcccg ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc
     4441 gaactgcccg ctgttctaca accggtcgcg gaggctatgg atgcgatcgc tgcggccgat
     4501 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     4561 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     4621 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     4681 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     4741 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     4801 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     4861 cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg ggcgtatatg
     4921 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     4981 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     5041 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     5101 gatagtggaa accgacgccc cagcactcgt ccgagggcaa agaaatagag tagatgccga
     5161 ccgggatctg tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     5221 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     5281 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     5341 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
     5401 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     5461 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     5521 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     5581 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     5641 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     5701 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     5761 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     5821 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     5881 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     5941 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     6001 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     6061 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     6121 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     6181 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     6241 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     6301 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     6361 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     6421 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     6481 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     6541 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     6601 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     6661 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
     6721 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6781 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6841 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6901 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6961 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7021 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7081 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7141 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7201 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7261 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7321 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7381 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7441 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7501 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     7561 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     7621 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     7681 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7741 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7801 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     7861 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     7921 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     7981 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     8041 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     8101 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     8161 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     8221 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     8281 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     8341 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     8401 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     8461 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     8521 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     8581 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     8641 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     8701 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     8761 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     8821 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     8881 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     8941 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     9001 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     9061 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     9121 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     9181 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     9241 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     9301 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     9361 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
     9421 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     9481 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     9541 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     9601 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     9661 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     9721 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     9781 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     9841 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     9901 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     9961 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    10021 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    10081 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    10141 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    10201 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    10261 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    10321 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    10381 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    10441 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    10501 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    10561 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    10621 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    10681 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    10741 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    10801 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    10861 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    10921 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    10981 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    11041 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    11101 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    11161 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    11221 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    11281 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    11341 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    11401 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    11461 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    11521 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    11581 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    11641 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    11701 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
    11761 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
    11821 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11881 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11941 cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcaa catggtggag
    12001 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
    12061 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
    12121 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
    12181 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
    12241 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
    12301 gtggattgat gtgataacat ggt
//
