LOCUS       sequence-189290-       11677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged mutated mouse FIT2 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Cai Y, McClinchie E, Price A, Nguyen TN, Gidda SK, Watt SC, 
            Yurchenko O, Park S, Sturtevant D, Mullen RT, Dyer JM, Chapman KD
  TITLE     Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes 
            lipid droplet accumulation in plants.
  JOURNAL   Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678.
  PUBMED    27987528
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678."
COMMENT     Sequence Label: pMDC43-FIT2-N[80]A
FEATURES             Location/Qualifiers
     source          1..11677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..350
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(418..446)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1041..1061
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(1911..2035)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2099..2351
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2368..2384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2368..2384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2381..2403)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2392..2408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2416..2422,2423..2440,2441..2446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2461..2482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2673..3349
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3416..4441
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4482..4656
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4734..4758
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5183..5977
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6064..6652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6553..6572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6806..6823
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6838..6978
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6904..6923)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7064..7086
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7322..7516
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7582..8655)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9084..9713)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11014..11038
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11226..11248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11240..11257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11241..11257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
       61 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
      121 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
      181 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      241 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
      301 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac ctcgactcta
      361 gaggatcccc gggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt
      421 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      481 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      541 aaactacctg ttccatggcc aaccctggtc accaccctga cctacggcgt gcagtgcttc
      601 tcccgttacc ctgatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga
      661 tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa
      721 gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag
      781 gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac
      841 atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc
      901 gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatggc
      961 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     1021 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     1081 ggcatggatg aactatacaa aggcgcgccc accatggatc acctggagcg ctgcgcgtgg
     1141 tttctccgcg ggacgctggt gagggcgacg gtgcggcgcc acctgccctg ggcgctggtg
     1201 gccgccatgc tggcgggctc ggtcgtcaag gagctgtccc cgctgcccga gagctacctc
     1261 agcaacaagc gcaacgtcct caacgtgtat tttgttaaat tggcctgggc ctggacagtc
     1321 tgtctcctcc tgcctttcat tgcccttacc gcataccacc tgacgggcaa gaccagcctg
     1381 gtcctgcggc ggttgagcac cctcctcgtg ggcacagcca tctggtatat ctgcacagcc
     1441 ctcttctcca acattgagca ctatacaggc agctgctacc agtccccggc cctggagggc
     1501 atcagacagg agcatcgcag caagcagcag tgccaccggg aagggggctt ctggcatggc
     1561 tttgacatct cgggccactc cttcctcttg accttctgtg cgctcatgat tgtggaagag
     1621 atggccgtgc tgcatgaggt gaagacggac cgcggccacc acctccatgc cgccatcacc
     1681 accctggttg tcgccctggg tttcctgacc ttcatctggg tgtggatgtt tctgtgcaca
     1741 gccgtttatt tccacgactt gacccagaaa gtgttcggca ccatgtttgg tttgttgggc
     1801 tggtacggaa catatgggta ttggtatctg aagtctttct ctccaggact tcctccccag
     1861 agttgtagtt tgactttgaa gcgagatact tacaagaaat gaacgtcgac catagtgact
     1921 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     1981 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttcgat
     2041 aattcttaat taactagttc tagagcggcc gccaccgcgg tggagctcga atttccccga
     2101 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     2161 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     2221 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     2281 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     2341 gttactagat cgggaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     2401 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2461 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2521 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2581 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
     2641 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     2701 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     2761 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     2821 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     2881 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
     2941 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     3001 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     3061 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     3121 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     3181 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     3241 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     3301 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
     3361 tctctctaca aatctatctc tctcgagctt tcgcagatcc ggggggcaat gagatatgaa
     3421 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     3481 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     3541 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     3601 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     3661 gtttagcgag agcctgacct attgcatctc ccgccgttca cagggtgtca cgttgcaaga
     3721 cctgcctgaa accgaactgc ccgctgttct acaaccggtc gcggaggcta tggatgcgat
     3781 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     3841 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     3901 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     3961 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     4021 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     4081 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     4141 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccacgact
     4201 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     4261 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     4321 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     4381 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaagaaata
     4441 gagtagatgc cgaccgggat ctgtcgatcg acaagctcga gtttctccat aataatgtgt
     4501 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     4561 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     4621 ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt
     4681 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     4741 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     4801 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     4861 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     4921 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     4981 ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     5041 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     5101 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     5161 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     5221 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     5281 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     5341 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     5401 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     5461 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     5521 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     5581 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     5641 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     5701 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     5761 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     5821 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     5881 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     5941 aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat
     6001 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6061 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6121 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6181 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6241 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6301 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6361 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6421 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6481 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6541 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6601 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6661 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6721 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6781 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     6841 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     6901 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     6961 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7021 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7081 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     7141 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     7201 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     7261 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     7321 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     7381 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     7441 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     7501 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     7561 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     7621 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     7681 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     7741 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     7801 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     7861 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     7921 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     7981 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     8041 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     8101 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     8161 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     8221 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     8281 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     8341 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     8401 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     8461 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     8521 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     8581 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     8641 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     8701 caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     8761 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     8821 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     8881 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     8941 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     9001 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     9061 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     9121 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     9181 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     9241 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     9301 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     9361 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     9421 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     9481 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     9541 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     9601 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     9661 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     9721 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     9781 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     9841 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     9901 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     9961 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    10021 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    10081 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    10141 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    10201 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    10261 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    10321 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    10381 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    10441 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    10501 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    10561 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    10621 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    10681 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    10741 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    10801 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    10861 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
    10921 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    10981 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
    11041 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta
    11101 gcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt
    11281 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga
    11341 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
    11401 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
    11461 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
    11521 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
    11581 gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacacac ttgtctactc
    11641 caaaaatatc aaagatacag tctcagaaga ccaaagg
//
