LOCUS       sequence-189305-       12966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express N-terminal truncated Arabidopsis SEIPIN3 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12966)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 12966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC84-SEIPIN3-Ndelta
FEATURES             Location/Qualifiers
     source          1..12966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        443..787
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1905..1933)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2528..2548
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2589..2606
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2626..2878
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2895..2911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2895..2911)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2908..2930)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2919..2935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2943..2949,2950..2967,2968..2973))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2988..3009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3200..3876
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3943..4968
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5009..5183
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5261..5285
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5710..6504
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7790..8378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8279..8298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8532..8549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8564..8704
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8630..8649)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8790..8812
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9048..9242
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9308..10381)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10810..11439)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12740..12764
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(12952..12966,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(12966,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt caacatggtg gagcacgaca
       61 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      121 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      181 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      241 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
      301 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      361 gatgtgataa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
      421 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
      481 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
      541 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
      601 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
      661 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      721 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      781 agaggacctc gactctagaa ctagttaatt aaatgattga atttcaagtt agtttgatga
      841 ttacctttat aaggtttcct ccatggttaa ttagtaaatg tttgagcttt gtttttgatc
      901 cgtatcgaac aatgaggcgt ggtagacgct atttagtatc gtggattgtt gggttatgtg
      961 attcagggtt aaaagatgat aagcctgtgt tggagcttgt gcgtagagta acatggggtt
     1021 tgttttgtgc tgtttatgtg gggatcatgt tgtttgcctt gttggtttct gcgtttatga
     1081 ttagtggttt tgtgattacc tatttggctc atgaaccact tgttatcaaa gagagcttga
     1141 attttgatta caccaagagt agtcctgaag cttatgtgcc cataagttca tgtgctggtg
     1201 ttgcctttgg tttgagtggc aaggagagta ttgaaactgg gaaggtcaaa gggttgaaag
     1261 acaggacaga gataactgtg tcaatgacat tgcctgagtc cgagtacaat agaaatctcg
     1321 ggatgtttca ggttcgggtg gacttcttat ccgcgagtgg tcatgtgctt gctagttcaa
     1381 ggcggccgtg tatggtaaaa tttagtagcg agcctatacg tcttgtccag actttgctaa
     1441 agattgctcc gcttgtcacg ggctatgtct ctgaaatcca aaccttgaac ttgaagctta
     1501 aaggcttggt cgaaaaggac attattccta ctgcttgctt gaagatcatg atcgaacaac
     1561 gtgcagagtt tcgtcctgga gcaggtattc ctgagattta cgatgcatct ctgttccttg
     1621 aatccaaact tcctttcttg aaaaggatca tatggaactg gaggaaaact ttgtttgtct
     1681 ggatcagcat gagtttgttc attatggaat tacttttcgc cttagttttc ttcagacctc
     1741 tgatcatccc aagaacaggt cagagaacac aacaaagaga tggaacacat tctatcaaca
     1801 acaacttata tcttgacggt caggcgggaa gtagatggcg cgcctcgagg gggggcccgg
     1861 taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     1921 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     1981 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2041 catggccaac cctggtcacc accctgacct acggcgtgca gtgcttctcc cgttaccctg
     2101 atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga
     2161 ggaccatctt cttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg
     2221 gagacaccct cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca
     2281 tcctcggcca caagttggaa tacaactaca actcccacaa cgtatacatc atggccgaca
     2341 agcaaaagaa cggcatcaaa gccaacttca agacccgcca caacatcgaa gacggcggcg
     2401 tgcaactcgc tgatcattat caacaaaata ctccaattgg cgatggccct gtccttttac
     2461 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     2521 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggtagaac
     2581 tatacaaaca ccaccaccac caccactaag agctcgaatt tccccgatcg ttcaaacatt
     2641 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2701 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2761 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     2821 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     2881 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2941 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3001 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
     3121 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3181 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3241 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     3301 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     3361 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3421 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
     3481 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     3541 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     3601 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     3661 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     3721 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     3781 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     3841 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
     3901 ctatctctct cgagctttcg cagatccggg gggcaatgag atatgaaaaa gcctgaactc
     3961 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     4021 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     4081 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     4141 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt tagcgagagc
     4201 ctgacctatt gcatctcccg ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc
     4261 gaactgcccg ctgttctaca accggtcgcg gaggctatgg atgcgatcgc tgcggccgat
     4321 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     4381 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     4441 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     4501 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     4561 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     4621 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     4681 cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg ggcgtatatg
     4741 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     4801 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     4861 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4921 gatagtggaa accgacgccc cagcactcgt ccgagggcaa agaaatagag tagatgccga
     4981 ccgggatctg tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     5041 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     5101 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     5161 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
     5221 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     5281 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     5341 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     5401 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     5461 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     5521 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     5581 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     5641 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     5701 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     5761 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     5821 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     5881 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     5941 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     6001 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     6061 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     6121 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     6181 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     6241 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     6301 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     6361 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     6421 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     6481 attttactgg atgaattgtt ttagtaccta gggaaggtgc gaataagcgg ggaaattctt
     6541 ctcggctgac tcagtcattt catttcttca tgtttgagcc gattttttct cccgtaaatg
     6601 ccttgaatca gcctatttag accgtttctt cgccatttaa ggcgttatcc ccagttttta
     6661 gtgagatctc tcccactgac gtatcatttg gtccgcccga aacaggttgg ccagcgtgaa
     6721 taacatcgcc agttggttat cgtttttcag caaccccttg tatctggctt tcacgaagcc
     6781 gaactgtcgc ttgatgatgc gaaatgggtg ctccaccctg gcccggatgc tggctttcat
     6841 gtattcgatg ttgatggccg ttttgttctt gcgtggatgc tgtttcaagg ttcttacctt
     6901 gccggggcgc tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg
     6961 cgccccttgg tagccggcat cggctgagac aaattgctcc tctccatgca gcagattacc
     7021 cagctgattg aggtcatgct cgttggccgc ggtggtgacc aggctgtggg tcaggccact
     7081 cttggcatcg acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt
     7141 ctgatgcatc tccggatcgc gttgctgctc tttgttcttg gtcgagctgg gtgcctcaat
     7201 gatggtggca tcgaccaagg tgccttgagt catcatgacg cctgcttcgg ccagccagcg
     7261 attgatggtc ttgaacaatt ggcgggccag ttgatgctgc tccagcaggt ggcggaaatt
     7321 catgatggtg gtgcggtccg gcaaggcgct atccagggat aaccgggcaa acagacgcat
     7381 ggaggcgatt tcgtacagag catcttccat cgcgccatcg ctcaggttgt accaatgctg
     7441 catgcagtga atgcgtagca tggtttccag cggataaggt cgccggccat taccagcctt
     7501 ggggtaaaac ggctcgatga cttccaccat gttttgccat ggcagaatct gctccatgcg
     7561 ggacaagaaa atctcttttc tggtctgacg gcgcttactg ctgaattcac tgtcggcgaa
     7621 ggtaagttga tgactcatga tgaaccctgt tctatggctc cagatgacaa acatgatctc
     7681 atatcaggga cttgttcgca ccttccctag aatgcatgac caaaatccct taacgtgagt
     7741 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7801 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7861 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7921 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7981 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8041 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8101 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8161 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8221 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8281 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8341 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8401 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8461 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8521 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     8581 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     8641 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     8701 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8761 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     8821 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     8881 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     8941 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     9001 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     9061 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     9121 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     9181 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     9241 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     9301 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     9361 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     9421 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     9481 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     9541 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     9601 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     9661 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     9721 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     9781 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     9841 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     9901 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     9961 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    10021 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    10081 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    10141 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    10201 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    10261 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    10321 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    10381 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
    10441 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    10501 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    10561 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    10621 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    10681 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    10741 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    10801 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    10861 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    10921 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    10981 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    11041 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    11101 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    11161 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    11221 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    11281 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    11341 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    11401 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    11461 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    11521 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    11581 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    11641 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    11701 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    11761 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    11821 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    11881 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    11941 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    12001 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    12061 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    12121 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    12181 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    12241 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    12301 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    12361 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    12421 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    12481 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    12541 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    12601 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    12661 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
    12721 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    12781 actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca
    12841 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    12901 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    12961 gacgtt
//
