LOCUS       sequence-189307-       12746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mutated Arabidopsis SEIPIN3 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 12746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC84-SEIPIN1N-3C
FEATURES             Location/Qualifiers
     source          1..12746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..19
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3..19
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        118..462
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1685..1713)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2308..2328
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2369..2386
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2406..2658
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2675..2691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2675..2691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2688..2710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2699..2715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2723..2729,2730..2747,2748..2753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2768..2789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2980..3658
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3725..4750
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4791..4965
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5043..5067
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5492..6286
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7572..8160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8061..8080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8314..8331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8346..8486
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8412..8431)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8572..8594
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8830..9024
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9090..10163)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10592..11221)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12522..12546
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(12734..12746,1..10)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcaacatgg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
      421 cccttcctct atataaggaa gttcatttca tttggagagg acctcgactc tagaactagt
      481 taattaaatg agaatcctcc aaaacaaaac aatgaaagaa caagacaacc aactcaaaat
      541 ccctgaacca ttacgagctg actggttcat ggttctagtc accatccaag ctgatattga
      601 atttcaagtt agtttgatga ttacctttat aaggtttcct ccatggttaa ttagtaaatg
      661 tttgagcttt gtttttgatc cgtatcgaac aatgaggcgt ggtagacgct atttagtatc
      721 gtggattgtt gggttatgtg attcagggtt aaaagatgat aagcctgtgt tggagcttgt
      781 gcgtagagta acatggggtt tgttttgtgc tgtttatgtg gggatcatgt tgtttgcctt
      841 gttggtttct gcgtttatga ttagtggttt tgtgattacc tatttggctc atgaaccact
      901 tgttatcaaa gagagcttga attttgatta caccaagagt agtcctgaag cttatgtgcc
      961 cataagttca tgtgctggtg ttgcctttgg tttgagtggc aaggagagta ttgaaactgg
     1021 gaaggtcaaa gggttgaaag acaggacaga gataactgtg tcaatgacat tgcctgagtc
     1081 cgagtacaat agaaatctcg ggatgtttca ggttcgggtg gacttcttat ccgcgagtgg
     1141 tcatgtgctt gctagttcaa ggcggccgtg tatggtaaaa tttagtagcg agcctatacg
     1201 tcttgtccag actttgctaa agattgctcc gcttgtcacg ggctatgtct ctgaaatcca
     1261 aaccttgaac ttgaagctta aaggcttggt cgaaaaggac attattccta ctgcttgctt
     1321 gaagatcatg atcgaacaac gtgcagagtt tcgtcctgga gcaggtattc ctgagattta
     1381 cgatgcatct ctgttccttg aatccaaact tcctttcttg aaaaggatca tatggaactg
     1441 gaggaaaact ttgtttgtct ggatcagcat gagtttgttc attatggaat tacttttcgc
     1501 cttagttttc ttcagacctc tgatcatccc aagaacaggt cagagaacac aacaaagaga
     1561 tggaacacat tctatcaaca acaacttata tcttgacggt caggcgggaa gtagatggcg
     1621 cgcctcgagg gggggcccgg taccggtaga aaaaatgagt aaaggagaag aacttttcac
     1681 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     1741 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     1801 tactggaaaa ctacctgttc catggccaac cctggtcacc accctgacct acggcgtgca
     1861 gtgcttctcc cgttaccctg atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc
     1921 tgagggatac gtgcaggaga ggaccatctt cttcaaggac gacgggaact acaagacacg
     1981 tgctgaagtc aagtttgagg gagacaccct cgtcaacagg atcgagctta agggaatcga
     2041 tttcaaggag gacggaaaca tcctcggcca caagttggaa tacaactaca actcccacaa
     2101 cgtatacatc atggccgaca agcaaaagaa cggcatcaaa gccaacttca agacccgcca
     2161 caacatcgaa gacggcggcg tgcaactcgc tgatcattat caacaaaata ctccaattgg
     2221 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     2281 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     2341 tacacatggc atggtagaac tatacaaaca ccaccaccac caccactaag agctcgaatt
     2401 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     2461 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     2521 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     2581 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     2641 catctatgtt actagatcgg gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     2701 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2761 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2821 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2881 tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg tctactccaa
     2941 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     3001 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     3061 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     3121 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3181 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg anancatggt
     3241 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     3301 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     3361 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     3421 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     3481 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     3541 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     3601 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa
     3661 tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatccg gggggcaatg
     3721 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     3781 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     3841 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     3901 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     3961 cattggggag tttagcgaga gcctgaccta ttgcatctcc cgccgttcac agggtgtcac
     4021 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttcta caaccggtcg cggaggctat
     4081 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     4141 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     4201 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     4261 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     4321 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     4381 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     4441 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     4501 gccacgactc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     4561 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     4621 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     4681 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     4741 aaagaaatag agtagatgcc gaccgggatc tgtcgatcga caagctcgag tttctccata
     4801 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     4861 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     4921 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg
     4981 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     5041 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     5101 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     5161 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     5221 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     5281 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
     5341 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     5401 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     5461 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     5521 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     5581 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     5641 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     5701 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     5761 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     5821 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     5881 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     5941 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     6001 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     6061 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     6121 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     6181 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     6241 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagggaaggt
     6301 gcgaataagc ggggaaattc ttctcggctg actcagtcat ttcatttctt catgtttgag
     6361 ccgatttttt ctcccgtaaa tgccttgaat cagcctattt agaccgtttc ttcgccattt
     6421 aaggcgttat ccccagtttt tagtgagatc tctcccactg acgtatcatt tggtccgccc
     6481 gaaacaggtt ggccagcgtg aataacatcg ccagttggtt atcgtttttc agcaacccct
     6541 tgtatctggc tttcacgaag ccgaactgtc gcttgatgat gcgaaatggg tgctccaccc
     6601 tggcccggat gctggctttc atgtattcga tgttgatggc cgttttgttc ttgcgtggat
     6661 gctgtttcaa ggttcttacc ttgccggggc gctcggcgat cagccagtcc acatccacct
     6721 cggccagctc ctcgcgctgt ggcgcccctt ggtagccggc atcggctgag acaaattgct
     6781 cctctccatg cagcagatta cccagctgat tgaggtcatg ctcgttggcc gcggtggtga
     6841 ccaggctgtg ggtcaggcca ctcttggcat cgacaccaat gtgggccttc atgccaaagt
     6901 gccactgatt gcctttcttg gtctgatgca tctccggatc gcgttgctgc tctttgttct
     6961 tggtcgagct gggtgcctca atgatggtgg catcgaccaa ggtgccttga gtcatcatga
     7021 cgcctgcttc ggccagccag cgattgatgg tcttgaacaa ttggcgggcc agttgatgct
     7081 gctccagcag gtggcggaaa ttcatgatgg tggtgcggtc cggcaaggcg ctatccaggg
     7141 ataaccgggc aaacagacgc atggaggcga tttcgtacag agcatcttcc atcgcgccat
     7201 cgctcaggtt gtaccaatgc tgcatgcagt gaatgcgtag catggtttcc agcggataag
     7261 gtcgccggcc attaccagcc ttggggtaaa acggctcgat gacttccacc atgttttgcc
     7321 atggcagaat ctgctccatg cgggacaaga aaatctcttt tctggtctga cggcgcttac
     7381 tgctgaattc actgtcggcg aaggtaagtt gatgactcat gatgaaccct gttctatggc
     7441 tccagatgac aaacatgatc tcatatcagg gacttgttcg caccttccct agaatgcatg
     7501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7681 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8221 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8281 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8341 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     8401 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     8461 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     8521 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8581 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     8641 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     8701 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     8761 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
     8821 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     8881 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     8941 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     9001 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
     9061 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     9121 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     9181 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     9241 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     9301 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     9361 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     9421 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     9481 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     9541 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     9601 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     9661 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     9721 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     9781 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     9841 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     9901 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     9961 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
    10021 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
    10081 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
    10141 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
    10201 agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
    10261 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
    10321 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
    10381 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
    10441 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
    10501 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
    10561 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
    10621 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
    10681 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
    10741 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
    10801 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
    10861 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
    10921 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
    10981 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
    11041 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
    11101 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
    11161 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
    11221 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
    11281 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
    11341 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
    11401 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
    11461 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
    11521 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
    11581 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
    11641 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
    11701 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
    11761 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
    11821 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
    11881 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    11941 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    12001 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    12061 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    12121 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    12181 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    12241 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    12301 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    12361 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
    12421 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
    12481 ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
    12541 ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag
    12601 ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    12661 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    12721 acgccagggt tttcccagtc acgacg
//
