LOCUS       sequence-18931-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     pBOB plasmids
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBOB-CAG-iCRE-SD
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    22..143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(33..52)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     87..106
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(273..647)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(666..713)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(828..847)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
       61 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      121 ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc
      181 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
      241 gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc
      301 acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc
      361 tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg
      421 gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc
      481 tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg
      541 tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg
      601 acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt ttagttcctc
      661 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
      721 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctangcctc
      781 caaaaaagcc tcctcactac ttctggaata gctcaganca gangcggcct cggcctctgc
      841 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcgga
//
