LOCUS       sequence-189312-        4960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible expression of Gsx2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Faedo A, Laporta A, Segnali A, Galimberti M, Besusso D, Cesana E, 
            Belloli S, Moresco RM, Tropiano M, Fuca E, Wild S, Bosio A, Vercelli
            AE, Biella G, Cattaneo E
  TITLE     Differentiation of human telencephalic progenitor cells into MSNs by
            inducible expression of Gsx2 and Ebf1.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1234-E1242. doi: 
            10.1073/pnas.1611473114. Epub 2017 Jan 30.
  PUBMED    28137879
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1611473114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-02-14- 14"; volume: "114"; issue: "7"; pages: 
            "E1234-E1242"
COMMENT     Sequence Label: pTRE3G-GSX2
FEATURES             Location/Qualifiers
     source          1..4960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(469..1044,1045..1047,1048..1056)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(637..654)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     864..883
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     intron          1229..1294
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1424..1444
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1869..1990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1906..1925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1960..1979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2076..2093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2247..2835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2327..2346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3006..3797,3798..3866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3629..3648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3867..3971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4039..4057
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4081..4459
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4089..4107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4125..4143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4161..4179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4197..4215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4233..4251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4269..4287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4305..4323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4368..4392
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4887..4904
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctctgggac acccgcagca ccacgcacct gtctgcgccg ccactaccta caacatgtcg
       61 gacccacgga gattccactg cctctccatg ggaggctccg ataccagcca ggtacccaat
      121 ggcaaaagga tgaggacagc gtttaccagc acgcagctcc tggagctgga gcgagaattc
      181 tcttccaata tgtacctgtc ccgactccgg agaatcgaga tcgcgacata cctaaacctg
      241 tcagagaagc aggtgaaaat ctggtttcag aaccgtcgcg tgaagcacaa gaaggagggg
      301 aaaggcgctt cgaggaacaa tcacacaagc tgcaagtgcg tgggtagcca ggcgcattat
      361 gcgcgctccg aggacgagga ctccttgtcc cctgcctcgg ctaacgaaga caaggagatt
      421 tcccccttgt aaaggcagag gctccttctg gatctatcga tcggccggcc cctctccctc
      481 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
      541 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
      601 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
      661 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
      721 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
      781 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
      841 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
      901 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta
      961 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     1021 tcctttgaaa aacacgatga taatatggcc acaaccgggc cggatatcac gcgtcatatg
     1081 gctagcctgc agggatccaa tgtaactgta ttcagcgatg acgaaattct tagctattgt
     1141 aatactctag aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     1201 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     1261 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     1321 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     1381 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     1441 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     1501 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     1561 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     1621 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     1681 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     1741 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     1801 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1861 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1981 atgtatctta tcatgtctgc ggctctagag ctgcattaat gaatcggcca acgcgcgggg
     2041 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2101 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2161 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2221 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2281 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2341 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2401 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2461 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2521 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2581 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2641 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2701 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2761 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2821 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2881 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2941 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3001 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3061 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     4021 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattcctc
     4081 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     4141 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     4201 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     4261 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     4321 agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
     4381 aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa ctttccgtac
     4441 cacttcctac cctcgtaaag tcgacaccgg ggcccagatc catcgacatg tcgcgctcct
     4501 tctacgtgga ctcactcatc atcaaggact cctcacggcc cgcaccctcg ctgccggaat
     4561 cgcacccagg gccggatttc ttcatcccgc tgggcatgcc gtccccgttg gtgatgtcag
     4621 tgtccgggcc tggctgtccg tcccgcaaaa gcggcgcttt ctgcgtgtgc ccgctttgcg
     4681 tcacctcgca cctgcactcc tctcgcccgc ccgcaggagc tggtggcgga gctacgggga
     4741 ccgcgggggc tgcagtggcg ggcggcgggg tggctggagg caccggcgct ctacctttgc
     4801 tcaaaagcca gttctctccc gctcctgggg acgcgcagtt ttgcccacgg gtgagccacg
     4861 cacaccatca ccaccaccca ccacagcatc atcatcacca tcaccagccc cagcagcccg
     4921 gctcggctgc agcggcggcc gcggcggcgg cggcggcagc
//
