LOCUS       sequence-189313-       12166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mouse FIT2 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Cai Y, McClinchie E, Price A, Nguyen TN, Gidda SK, Watt SC, 
            Yurchenko O, Park S, Sturtevant D, Mullen RT, Dyer JM, Chapman KD
  TITLE     Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes 
            lipid droplet accumulation in plants.
  JOURNAL   Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678.
  PUBMED    27987528
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678."
COMMENT     Sequence Label: pMDC32-FIT2-N[80]A
FEATURES             Location/Qualifiers
     source          1..12166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        444..788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    complement(1639..1763)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1827..2079
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2096..2112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2096..2112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2109..2131)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2120..2136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2144..2150,2151..2168,2169..2174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2189..2210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2401..3077
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3144..4169
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4210..4384
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4462..4486
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4911..5705
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6991..7579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7480..7499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7733..7750
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7765..7905
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7831..7850)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7991..8013
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8249..8443
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8509..9582)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10011..10640)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11941..11965
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(12153..12166,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac
       61 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
      121 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
      181 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      241 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
      301 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      361 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
      421 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
      481 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
      541 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
      601 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
      661 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
      721 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
      781 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgcccac
      841 catggatcac ctggagcgct gcgcgtggtt tctccgcggg acgctggtga gggcgacggt
      901 gcggcgccac ctgccctggg cgctggtggc cgccatgctg gcgggctcgg tcgtcaagga
      961 gctgtccccg ctgcccgaga gctacctcag caacaagcgc aacgtcctca acgtgtattt
     1021 tgttaaattg gcctgggcct ggacagtctg tctcctcctg cctttcattg cccttaccgc
     1081 ataccacctg acgggcaaga ccagcctggt cctgcggcgg ttgagcaccc tcctcgtggg
     1141 cacagccatc tggtatatct gcacagccct cttctccaac attgagcact atacaggcag
     1201 ctgctaccag tccccggccc tggagggcgt cagacaggag catcgcagca agcagcagtg
     1261 ccaccgggaa gggggcttct ggcatggctt tgacatctcg ggccactcct tcctcttgac
     1321 cttctgtgcg ctcatgattg tggaagagat ggccgtgctg catgaggtga agacggaccg
     1381 cggccaccac ctccatgccg ccatcaccac cctggttgtc gccctgggtt tcctgacctt
     1441 catctgggtg tggatgtttc tgtgcacagc cgtttatttc cacgacttga cccagaaagt
     1501 gttcggcacc atgtttggtt tgttgggctg gtacggaaca tatgggtatt ggtatctgaa
     1561 gtctttctct ccaggacttc ctccccagag ttgtagtttg actttgaagc gagatactta
     1621 caagaaatga acgtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     1681 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     1741 tcagctttct tgtacaaagt ggttcgataa ttcttaatta actagttcta gagcggccgc
     1801 caccgcggtg gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     1861 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     1921 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     1981 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     2041 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattcgta atcatgtcat
     2101 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2161 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     2221 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2281 aacgcgcggg gagaggcggt ttgcgtattg gctagagcag cttgccaaca tggtggagca
     2341 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
     2401 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
     2461 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
     2521 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
     2581 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     2641 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     2701 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
     2761 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
     2821 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
     2881 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2941 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     3001 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     3061 tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc tcgagctttc
     3121 gcagatccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     3181 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     3241 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3301 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     3361 cgattccgga agtgcttgac attggggagt ttagcgagag cctgacctat tgcatctccc
     3421 gccgttcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctac
     3481 aaccggtcgc ggaggctatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     3541 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     3601 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     3661 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3721 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     3781 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     3841 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     3901 atccggagct tgcaggatcg ccacgactcc gggcgtatat gctccgcatt ggtcttgacc
     3961 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     4021 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4081 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     4141 ccagcactcg tccgagggca aagaaataga gtagatgccg accgggatct gtcgatcgac
     4201 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     4261 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     4321 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     4381 gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     4441 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     4501 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     4561 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     4621 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     4681 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     4741 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     4801 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     4861 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     4921 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     4981 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     5041 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     5101 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     5161 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     5221 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     5281 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     5341 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     5401 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     5461 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     5521 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     5581 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     5641 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     5701 tttagtacct agggaaggtg cgaataagcg gggaaattct tctcggctga ctcagtcatt
     5761 tcatttcttc atgtttgagc cgattttttc tcccgtaaat gccttgaatc agcctattta
     5821 gaccgtttct tcgccattta aggcgttatc cccagttttt agtgagatct ctcccactga
     5881 cgtatcattt ggtccgcccg aaacaggttg gccagcgtga ataacatcgc cagttggtta
     5941 tcgtttttca gcaacccctt gtatctggct ttcacgaagc cgaactgtcg cttgatgatg
     6001 cgaaatgggt gctccaccct ggcccggatg ctggctttca tgtattcgat gttgatggcc
     6061 gttttgttct tgcgtggatg ctgtttcaag gttcttacct tgccggggcg ctcggcgatc
     6121 agccagtcca catccacctc ggccagctcc tcgcgctgtg gcgccccttg gtagccggca
     6181 tcggctgaga caaattgctc ctctccatgc agcagattac ccagctgatt gaggtcatgc
     6241 tcgttggccg cggtggtgac caggctgtgg gtcaggccac tcttggcatc gacaccaatg
     6301 tgggccttca tgccaaagtg ccactgattg cctttcttgg tctgatgcat ctccggatcg
     6361 cgttgctgct ctttgttctt ggtcgagctg ggtgcctcaa tgatggtggc atcgaccaag
     6421 gtgccttgag tcatcatgac gcctgcttcg gccagccagc gattgatggt cttgaacaat
     6481 tggcgggcca gttgatgctg ctccagcagg tggcggaaat tcatgatggt ggtgcggtcc
     6541 ggcaaggcgc tatccaggga taaccgggca aacagacgca tggaggcgat ttcgtacaga
     6601 gcatcttcca tcgcgccatc gctcaggttg taccaatgct gcatgcagtg aatgcgtagc
     6661 atggtttcca gcggataagg tcgccggcca ttaccagcct tggggtaaaa cggctcgatg
     6721 acttccacca tgttttgcca tggcagaatc tgctccatgc gggacaagaa aatctctttt
     6781 ctggtctgac ggcgcttact gctgaattca ctgtcggcga aggtaagttg atgactcatg
     6841 atgaaccctg ttctatggct ccagatgaca aacatgatct catatcaggg acttgttcgc
     6901 accttcccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6961 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7261 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7381 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7441 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7501 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7561 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7621 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7681 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7741 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7801 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7861 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     7921 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7981 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8041 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     8101 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     8161 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     8221 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     8281 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     8341 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     8401 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     8461 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     8521 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     8581 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     8641 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     8701 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     8761 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     8821 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     8881 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     8941 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     9001 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     9061 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     9121 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     9181 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     9241 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     9301 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     9361 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     9421 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     9481 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     9541 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     9601 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
     9661 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     9721 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     9781 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     9841 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     9901 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     9961 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    10021 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    10081 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    10141 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    10201 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    10261 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    10321 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    10381 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    10441 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    10501 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    10561 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    10621 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    10681 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    10741 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    10801 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    10861 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    10921 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    10981 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    11041 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    11101 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    11161 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    11221 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    11281 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    11341 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    11401 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    11461 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    11521 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    11581 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    11641 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    11701 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    11761 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    11821 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    11881 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
    11941 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
    12001 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
    12061 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    12121 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgt
//
