LOCUS       sequence-189318-       11025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged mutated mouse FIT2 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Cai Y, McClinchie E, Price A, Nguyen TN, Gidda SK, Watt SC, 
            Yurchenko O, Park S, Sturtevant D, Mullen RT, Dyer JM, Chapman KD
  TITLE     Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes 
            lipid droplet accumulation in plants.
  JOURNAL   Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678.
  PUBMED    27987528
REFERENCE   2  (bases 1 to 11025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678."
COMMENT     Sequence Label: pMDC43-FIT2-FLL[157-159]AAA
FEATURES             Location/Qualifiers
     source          1..11025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        117..461
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(529..557)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1152..1172
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(2022..2146)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2210..2462
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2479..2495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2479..2495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2492..2514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2503..2519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2527..2533,2534..2551,2552..2557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2572..2593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2784..3129
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3202..4227
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4268..4442
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4520..4544
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4969..5763
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5850..6438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6339..6358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6592..6609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6624..6764
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6690..6709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6850..6872
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7108..7302
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7368..8441)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8870..9499)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10800..10824
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(11012..11025,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac
       61 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
      121 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
      181 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      241 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
      301 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      361 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
      421 ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct agaggatccc
      481 cgggtaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt
      541 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      601 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      661 gttccatggc caaccctggt caccaccctg acctacggcg tgcagtgctt ctcccgttac
      721 cctgatcata tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag
      781 gagaggacca tcttcttcaa ggacgacggg aactacaaga cacgtgctga agtcaagttt
      841 gagggagaca ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga
      901 aacatcctcg gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc
      961 gacaagcaaa agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc
     1021 ggcgtgcaac tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     1081 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     1141 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1201 gaactataca aaggcgcgcc caccatggat cacctggagc gctgcgcgtg gtttctccgc
     1261 gggacgctgg tgagggcgac ggtgcggcgc cacctgccct gggcgctggt ggccgccatg
     1321 ctggcgggct cggtcgtcaa ggagctgtcc ccgctgcccg agagctacct cagcaacaag
     1381 cgcaacgtcc tcaacgtgta ttttgttaaa ttggcctggg cctggacagt ctgtctcctc
     1441 ctgcctttca ttgcccttac caactaccac ctgacgggca agaccagcct ggtcctgcgg
     1501 cggttgagca ccctcctcgt gggcacagcc atctggtata tctgcacagc cctcttctcc
     1561 aacattgagc actatacagg cagctgctac cagtccccgg ccctggaggg catcagacag
     1621 gagcatcgca gcaagcagca gtgccaccgg gaagggggct tctggcatgg ctttgacatc
     1681 tcgggccact ccgcagcagc aaccttctgt gcgctcatga ttgtggaaga gatggccgtg
     1741 ctgcatgagg tgaagacgga ccgcggccac cacctccatg ccgccatcac caccctggtt
     1801 gtcgccctgg gtttcctgac cttcatctgg gtgtggatgt ttctgtgcac agccgtttat
     1861 ttccacgact tgacccagaa agtgttcggc accatgtttg gtttgttggg ctggtacgga
     1921 acatatgggt attggtatct gaagtctttc tctccaggac ttcctcccca gagttgtagt
     1981 ttgactttga agcgagatac ttacaagaaa tgaacgtcga ccatagtgac tggatatgtt
     2041 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     2101 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttcga taattcttaa
     2161 ttaactagtt ctagagcggc cgccaccgcg gtggagctcg aatttccccg atcgttcaaa
     2221 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2281 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     2341 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     2401 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     2461 tcgggaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
     2701 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     2761 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     2821 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     2881 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     2941 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     3001 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     3061 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     3121 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc
     3181 agatccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     3241 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     3301 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     3361 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     3421 attccggaag tgcttgacat tggggagttt agcgagagcc tgacctattg catctcccgc
     3481 cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctacaa
     3541 ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     3601 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     3661 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     3721 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     3781 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     3841 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     3901 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     3961 ccggagcttg caggatcgcc acgactccgg gcgtatatgc tccgcattgg tcttgaccaa
     4021 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     4081 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     4141 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     4201 agcactcgtc cgagggcaaa gaaatagagt agatgccgac cgggatctgt cgatcgacaa
     4261 gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta
     4321 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     4381 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga
     4441 tcccccgaat taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     4501 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     4561 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     4621 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     4681 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     4741 atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg
     4801 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
     4861 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
     4921 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
     4981 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
     5041 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
     5101 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
     5161 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
     5221 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
     5281 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
     5341 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
     5401 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca
     5461 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
     5521 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
     5581 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
     5641 gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
     5701 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
     5761 tagtacctag aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5821 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5881 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5941 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6001 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6061 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6241 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6301 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6361 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6421 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6481 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6541 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6601 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     6661 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6721 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     6781 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6841 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6901 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     6961 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     7021 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     7081 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     7141 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     7201 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     7261 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     7321 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     7381 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     7441 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     7501 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     7561 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     7621 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     7681 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     7741 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     7801 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     7861 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     7921 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     7981 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     8041 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     8101 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     8161 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
     8221 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
     8281 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
     8341 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
     8401 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
     8461 gggttccagt gccattgcag ggccggcagg caacccagcc gcttacgcct ggccaaccgc
     8521 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     8581 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     8641 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     8701 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
     8761 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
     8821 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
     8881 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
     8941 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
     9001 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
     9061 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
     9121 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
     9181 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
     9241 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
     9301 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
     9361 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
     9421 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
     9481 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
     9541 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
     9601 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
     9661 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
     9721 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
     9781 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
     9841 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
     9901 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
     9961 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    10021 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    10081 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    10141 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    10201 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    10261 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    10321 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    10381 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    10441 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    10501 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    10561 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    10621 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    10681 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    10741 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
    10801 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
    10861 atctgatcca agctcaagct gctctagcat tcgccattca ggctgcgcaa ctgttgggaa
    10921 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    10981 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgt
//
