LOCUS       sequence-189319-       11350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged mouse FIT2 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Cai Y, McClinchie E, Price A, Nguyen TN, Gidda SK, Watt SC, 
            Yurchenko O, Park S, Sturtevant D, Mullen RT, Dyer JM, Chapman KD
  TITLE     Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes 
            lipid droplet accumulation in plants.
  JOURNAL   Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678.
  PUBMED    27987528
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678."
COMMENT     Sequence Label: pMDC43-FIT2
FEATURES             Location/Qualifiers
     source          1..11350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        117..461
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(529..557)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1152..1172
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(2022..2146)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2210..2462
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2479..2495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2479..2495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2492..2514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2503..2519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2527..2533,2534..2551,2552..2557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2572..2593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2784..3460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3527..4552
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4593..4767
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4845..4869
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5294..6088
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6175..6763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6664..6683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6917..6934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6949..7089
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7015..7034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7175..7197
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7433..7627
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7693..8766)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9195..9824)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11125..11149
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(11337..11350,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac
       61 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
      121 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
      181 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      241 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
      301 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      361 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
      421 ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct agaggatccc
      481 cgggtaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt
      541 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      601 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      661 gttccatggc caaccctggt caccaccctg acctacggcg tgcagtgctt ctcccgttac
      721 cctgatcata tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag
      781 gagaggacca tcttcttcaa ggacgacggg aactacaaga cacgtgctga agtcaagttt
      841 gagggagaca ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga
      901 aacatcctcg gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc
      961 gacaagcaaa agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc
     1021 ggcgtgcaac tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     1081 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     1141 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1201 gaactataca aaggcgcgcc caccatggat cacctggagc gctgcgcgtg gtttctccgc
     1261 gggacgctgg tgagggcgac ggtgcggcgc cacctgccct gggcgctggt ggccgccatg
     1321 ctggcgggct cggtcgtcaa ggagctgtcc ccgctgcccg agagctacct cagcaacaag
     1381 cgcaacgtcc tcaacgtgta ttttgttaaa ttggcctggg cctggacagt ctgtctcctc
     1441 ctgcctttca ttgcccttac caactaccac ctgacgggca agaccagcct ggtcctgcgg
     1501 cggttgagca ccctcctcgt gggcacagcc atctggtata tctgcacagc cctcttctcc
     1561 aacattgagc actatacagg cagctgctac cagtccccgg ccctggaggg catcagacag
     1621 gagcatcgca gcaagcagca gtgccaccgg gaagggggct tctggcatgg ctttgacatc
     1681 tcgggccact ccttcctctt gaccttctgt gcgctcatga ttgtggaaga gatggccgtg
     1741 ctgcatgagg tgaagacgga ccgcggccac cacctccatg ccgccatcac caccctggtt
     1801 gtcgccctgg gtttcctgac cttcatctgg gtgtggatgt ttctgtgcac agccgtttat
     1861 ttccacgact tgacccagaa agtgttcggc accatgtttg gtttgttggg ctggtacgga
     1921 acatatgggt attggtatct gaagtctttc tctccaggac ttcctcccca gagttgtagt
     1981 ttgactttga agcgagatac ttacaagaaa tgaacgtcga ccatagtgac tggatatgtt
     2041 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     2101 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttcga taattcttaa
     2161 ttaactagtt ctagagcggc cgccaccgcg gtggagctcg aatttccccg atcgttcaaa
     2221 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2281 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     2341 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     2401 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     2461 tcgggaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
     2701 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     2761 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     2821 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     2881 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     2941 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     3001 ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt
     3061 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     3121 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     3181 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     3241 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     3301 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     3361 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     3421 tatataagga agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac
     3481 aaatctatct ctctcgagct ttcgcagatc cggggggcaa tgagatatga aaaagcctga
     3541 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     3601 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3661 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3721 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agtttagcga
     3781 gagcctgacc tattgcatct cccgccgttc acagggtgtc acgttgcaag acctgcctga
     3841 aaccgaactg cccgctgttc tacaaccggt cgcggaggct atggatgcga tcgctgcggc
     3901 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3961 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     4021 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     4081 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     4141 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     4201 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     4261 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccacgac tccgggcgta
     4321 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     4381 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     4441 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     4501 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat agagtagatg
     4561 ccgaccggga tctgtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc
     4621 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
     4681 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     4741 ccaaaatcca gtactaaaat ccagatcccc cgaattaatt cggcgttaat tcagtacatt
     4801 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     4861 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     4921 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     4981 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     5041 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     5101 gtgatcaccg cggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     5161 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     5221 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     5281 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     5341 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     5401 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     5461 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     5521 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     5581 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     5641 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     5701 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     5761 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     5821 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     5881 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     5941 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     6001 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     6061 ttatatttta ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg
     6121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6301 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6841 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6901 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     6961 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     7021 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     7081 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7141 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7201 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     7261 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     7321 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     7381 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     7441 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     7501 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     7561 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     7621 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     7681 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     7741 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     7801 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     7861 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     7921 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     7981 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     8041 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     8101 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     8161 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     8221 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     8281 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     8341 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     8401 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     8461 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     8521 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     8581 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     8641 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     8701 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     8761 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc
     8821 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     8881 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     8941 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     9001 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     9061 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     9121 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     9181 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     9241 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     9301 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     9361 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     9421 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     9481 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     9541 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     9601 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     9661 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     9721 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     9781 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     9841 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     9901 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     9961 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    10021 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    10081 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    10141 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    10201 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    10261 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    10321 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    10381 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    10441 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    10501 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    10561 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    10621 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    10681 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    10741 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    10801 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    10861 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    10921 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    10981 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
    11041 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
    11101 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
    11161 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct agcattcgcc
    11221 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    11281 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    11341 gtcacgacgt
//
