LOCUS       sequence-18932-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Marr RA, Guan H, Rockenstein E, Kindy M, Gage FH, Verma I, Masliah 
            E, Hersh LB
  TITLE     Neprilysin regulates amyloid Beta peptide levels.
  JOURNAL   J Mol Neurosci. 2004  . 22(1-2):5-11.
  PUBMED    14742905
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Neurosci. 2004  . 22(1-2):5-11."
COMMENT     Sequence Label: pCSC-SP-PW (aka: pBOB)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    2..83
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     27..46
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(213..587)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(606..653)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(770..789)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
       61 ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc
      121 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
      181 gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc
      241 acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc
      301 tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg
      361 gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc
      421 tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg
      481 tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg
      541 acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt ttagttcctc
      601 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
      661 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc
      721 caaaaaagcc tcctcactac ttctggaata gctcagaggc agagggcggc ctcggcctct
      781 gcataaataa aaaaaattag tcagccatgg gggcggaaaa atgg
//
