LOCUS       sequence-189335-        6044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: B108-Otx2-IRES-Cre
FEATURES             Location/Qualifiers
     source          1..6044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(161..178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     388..407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             581..1609
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1619..1648
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1744..1763)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1809..1864
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1863..1882)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2225..2241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2225..2241)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2238..2260)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2249..2265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2273..2279,2280..2297,2298..2303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2318..2339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2398..2593
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2444..2579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2599..2733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2636..2655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2690..2709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2801..2818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2972..3560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3052..3071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3731..4522,4523..4591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4354..4373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4592..4696)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4769..4817
                     /note="synthetic polyadenylation signal"
     misc_feature    4831..4922
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4871..4890
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     misc_feature    join(6038..6044,1..568,569..571,572..580)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
       61 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
      121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
      181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
      241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
      301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
      361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
      421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
      481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      541 gttttccttt gaaaaacacg atgataatat ggccacaacc atggccaatt tactgaccgt
      601 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
      661 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
      721 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
      781 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
      841 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
      901 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
      961 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
     1021 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
     1081 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
     1141 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
     1201 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
     1261 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
     1321 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
     1381 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
     1441 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     1501 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
     1561 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatg gaccggtgga
     1621 acaaaaactt atttctgaag aagatctgtg aatcgcggcc ggccgcactc ctcaggtgca
     1681 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     1741 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     1801 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     1861 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     1921 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     1981 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     2041 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     2101 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     2161 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     2221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     2401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     2461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     2521 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     2581 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2701 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     2761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3421 ttggtatctg cgctctgctg aagtcagtta ccttcggaaa aagagttggt agctcttgat
     3481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaga agtaatatta
     4741 aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg
     4801 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccgtcaaa acaaaacgaa
     4861 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     4921 ctatcgatag tcgaccaatt gctcgagttc ggaaataggt caggtacttg ggagattaga
     4981 caagcttgct tcatccctga atacaattag gaggaattag acctgcagca agtagcttgg
     5041 atttatcagc tttaattgaa ttctatataa tggaagcttg accggagccg ttagttgccc
     5101 gtcggacgcg atttgcgagg cgctttatgc gcgcaccggt cgccaccatg atgtcttatc
     5161 taaagcaacc gccttacgca gtcaatgggc tgagtctgac cacttcgggt atggacttgc
     5221 tgcatccctc cgtgggctac cccgccaccc cccggaaaca gcgaagggag aggacgacat
     5281 ttactagggc acagctcgac gttctggaag ctctgtttgc caagacccgg tacccagaca
     5341 tcttcatgag ggaagaggtg gcactgaaaa tcaacttgcc agaatccagg gtgcaggtat
     5401 ggtttaagaa tcgaagagct aagtgccgcc aacagcagca gcagcagcag aatggaggtc
     5461 agaacaaagt gaggcctgcc aagaagaaga gctctccagc tcgggaagtg agttcagaga
     5521 gtggaacaag tggccagttc agtcccccct ctagtacctc agtcccaacc attgccagca
     5581 gcagtgctcc agtgtctatc tggagcccag cgtccatctc cccactgtct gaccccttgt
     5641 ccacttcctc ctcctgcatg cagaggtcct atcccatgac ctatactcag gcttcaggtt
     5701 atagtcaagg ctatgctggc tcaacttcct actttggggg catggactgt ggatcttatt
     5761 tgacccctat gcatcaccag cttcctggac caggggccac actcagtccc atgggtacca
     5821 atgctgttac cagccatctc aatcagtccc cagcttctct ttccacccag ggatatggag
     5881 cttcaagctt gggttttaac tcaaccactg attgcttgga ttataaggac caaactgcct
     5941 cttggaagct taacttcaat gctgactgct tggattataa agatcagacg tcctcatgga
     6001 aattccaggt tttgtgagta aattccggcc cccccccccc ctct
//
