LOCUS       sequence-189336-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression vector for Trx1 CSAAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Schwertassek U, Balmer Y, Gutscher M, Weingarten L, Preuss M, 
            Engelhard J, Winkler M, Dick TP
  TITLE     Selective redox regulation of cytokine receptor signaling by 
            extracellular thioredoxin-1.
  JOURNAL   EMBO J. 2007 Jul 11;26(13):3086-97. Epub 2007 Jun 7.
  PUBMED    17557078
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2007-07-11"; volume: "26(13)"; pages: "3086-97. Epub 2007 Jun
            7"
COMMENT     Sequence Label: pQE60 Trx1 CSAAA
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(16..30,31..36,37..53,54..60)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    68..84
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..114
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             442..555
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             562..579
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      602..696
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(806..825)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1468..1554
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1601..1623)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1709..1849
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1764..1783
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1864..1881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2035..2623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2115..2134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2794..3585,3586..3654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3417..3436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3655..3759)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3827..3845
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcacctcg agaaatcata aaaaatttat ttgctttgtg agcggataac aattataata
       61 gattcaattg tgagcggata acaatttcac acagaattca ttaaagagga gaaattaacc
      121 atggtgaagc agatcgaaag caagactgct tttcaggaag ccttggacgc tgcaggtgac
      181 aaacttgtag tagttgactt ctcagccacg tggtgtgggc ctagcaaaat gatcaagcct
      241 ttctttcatt ccctctctga aaagtattcc aacgtgatat tccttgaagt agatgtggat
      301 gacgctcagg atgttgcttc agaggctgaa gtcaaagcca tgccaacatt ccagtttttt
      361 aagaagggac aaaaggtggg tgaattttct ggagccaata aggaaaagct tgaagccacc
      421 attaatgaat tagtcagatc tatggacgaa aagaccaccg gctggcgcgg cggccacgtg
      481 gtggaaggcc tggccggcga actggaacag ctgcgcgccc gcctggaaca ccacccacag
      541 ggccagcgcg aaccaggatc tcatcaccat caccatcact aagcttaatt agctgagctt
      601 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
      661 ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagcttgg cgagattttc
      721 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      781 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      841 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      901 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
      961 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     1021 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     1081 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     1141 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     1201 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatgca
     1261 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1321 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1381 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     1441 gggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1501 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccc
     1561 tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     1621 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     1681 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     1741 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     1801 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     1861 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1921 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1981 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2041 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2101 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2161 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2221 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2281 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2341 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2401 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2461 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2521 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3781 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3841 gaggcccttt cgt
//
