LOCUS       sequence-189337-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression vector for Trx1 CCAAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Schwertassek U, Balmer Y, Gutscher M, Weingarten L, Preuss M, 
            Engelhard J, Winkler M, Dick TP
  TITLE     Selective redox regulation of cytokine receptor signaling by 
            extracellular thioredoxin-1.
  JOURNAL   EMBO J. 2007 Jul 11;26(13):3086-97. Epub 2007 Jun 7.
  PUBMED    17557078
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2007-07-11"; volume: "26(13)"; pages: "3086-97. Epub 2007 Jun
            7"
COMMENT     Sequence Label: pQE60 Trx1 CCAAA
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(8..22,23..28,29..45,46..52)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    28..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             95..106
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             434..547
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             554..571
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      594..688
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(798..817)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1460..1546
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1593..1615)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1701..1841
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1756..1775
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1856..1873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2027..2615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2107..2126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2786..3577,3578..3646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3409..3428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3647..3751)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3819..3837
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgagaaatca taaaaaattt atttgctttg tgagcggata acaattataa tagattcaat
       61 tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattaa ccatggtgaa
      121 gcagatcgaa agcaagactg cttttcagga agccttggac gctgcaggtg acaaacttgt
      181 agtagttgac ttctcagcca cgtggtgtgg gccttgcaaa atgatcaagc ctttctttca
      241 ttccctctct gaaaagtatt ccaacgtgat attccttgaa gtagatgtgg atgacgctca
      301 ggatgttgct tcagaggctg aagtcaaagc catgccaaca ttccagtttt ttaagaaggg
      361 acaaaaggtg ggtgaatttt ctggagccaa taaggaaaag cttgaagcca ccattaatga
      421 attagtcaga tctatggacg aaaagaccac cggctggcgc ggcggccacg tggtggaagg
      481 cctggccggc gaactggaac agctgcgcgc ccgcctggaa caccacccac agggccagcg
      541 cgaaccagga tctcatcacc atcaccatca ctaagcttaa ttagctgagc ttggactcct
      601 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      661 ccgccgggcg ttttttattg gtgagaatcc aagctagctt ggcgagattt tcaggagcta
      721 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      781 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      841 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      901 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
      961 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     1021 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1081 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     1141 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     1201 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg catgggcaaa
     1261 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     1321 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1381 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg
     1441 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1501 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cctctagagc
     1561 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     1621 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1681 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     1741 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     1801 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     1861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2461 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3781 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     3841 ttcgtcttca cct
//
