LOCUS       sequence-189346-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression vector for Trx1 SSAAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Schwertassek U, Balmer Y, Gutscher M, Weingarten L, Preuss M, 
            Engelhard J, Winkler M, Dick TP
  TITLE     Selective redox regulation of cytokine receptor signaling by 
            extracellular thioredoxin-1.
  JOURNAL   EMBO J. 2007 Jul 11;26(13):3086-97. Epub 2007 Jun 7.
  PUBMED    17557078
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2007-07-11"; volume: "26(13)"; pages: "3086-97. Epub 2007 Jun
            7"
COMMENT     Sequence Label: pQE60 Trx1 SSAAA
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(9..23,24..29,30..46,47..53)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    29..45
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    61..77
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             96..107
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             435..548
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             555..572
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      595..689
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(799..818)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1461..1547
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1594..1616)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1702..1842
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1757..1776
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1857..1874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2028..2616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2108..2127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2787..3578,3579..3647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3410..3429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3648..3752)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3820..3838
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgagaaatc ataaaaaatt tatttgcttt gtgagcggat aacaattata atagattcaa
       61 ttgtgagcgg ataacaattt cacacagaat tcattaaaga ggagaaatta accatggtga
      121 agcagatcga aagcaagact gcttttcagg aagccttgga cgctgcaggt gacaaacttg
      181 tagtagttga cttctcagcc acgtggagtg ggccttccaa aatgatcaag cctttctttc
      241 attccctctc tgaaaagtat tccaacgtga tattccttga agtagatgtg gatgacgctc
      301 aggatgttgc ttcagaggct gaagtcaaag ccatgccaac attccagttt tttaagaagg
      361 gacaaaaggt gggtgaattt tctggagcca ataaggaaaa gcttgaagcc accattaatg
      421 aattagtcag atctatggac gaaaagacca ccggctggcg cggcggccac gtggtggaag
      481 gcctggccgg cgaactggaa cagctgcgcg cccgcctgga acaccaccca cagggccagc
      541 gcgaaccagg atctcatcac catcaccatc actaagctta attagctgag cttggactcc
      601 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      661 gccgccgggc gttttttatt ggtgagaatc caagctagct tggcgagatt ttcaggagct
      721 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      781 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      841 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      901 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      961 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1021 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1081 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1141 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1201 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gcatgggcaa
     1261 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     1321 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     1381 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggggtaat
     1441 gactctctag cttgaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1501 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccctctagag
     1561 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     1621 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1681 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     1741 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     1801 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     1861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2461 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2701 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2761 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2821 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2881 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2941 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3001 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3061 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3121 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3181 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3241 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3301 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3361 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3421 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3481 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3541 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3601 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3661 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3721 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3781 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3841 tttcgtcttc acc
//
