LOCUS       sequence-189393-        9893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of MBP-tagged clathrin heavy chain large 
            fragment including N-terminal domain, his tagged at C-terminus for 
            purification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ
  TITLE     New tools for ""hot-wiring"" clathrin-mediated endocytosis with 
            temporal and spatial precision.
  JOURNAL   J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 
            10.1083/jcb.201702188.
  PUBMED    28954824
REFERENCE   2  (bases 1 to 9893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188."
COMMENT     Sequence Label: MBP-CHC(1-1074)-His6
FEATURES             Location/Qualifiers
     source          1..9893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2700
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             5792..5800
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5953..5970
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5983..6000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5983..5999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5992..6014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6178..6195)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6348..6434
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6526..6553
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6573..6664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6665..6733,6734..7525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6883..6902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7567..8080)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7762..7783)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7974..7993
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      8191..8779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8680..8699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8933..8950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8965..9107
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9033..9052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9193..9215
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9209..9400)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggctgg aagttctgtt ccaggggccc
     2701 gaattcggga tctgcatggc ccagattctg ccaattcgtt ttcaggagca tctccagctc
     2761 cagaacctgg gtatcaaccc agcaaacatt ggcttcagta ccctgactat ggagtctgac
     2821 aaattcatct gcattagaga aaaagtagga gagcaggccc aggtggtaat cattgatatg
     2881 aatgacccaa gtaatccaat tcgaagacca atttcagcag acagcgccat catgaatcca
     2941 gctagcaaag taattgcact gaaagctggg aaaactcttc agatttttaa cattgaaatg
     3001 aaaagtaaaa tgaaggctca taccatgact gatgatgtca ccttttggaa atggatctct
     3061 ttgaatacgg ttgctcttgt tacggataat gcagtttatc actggagtat ggaaggagag
     3121 tctcagccag tgaaaatgtt tgatcgccat tctagccttg cagggtgcca gattatcaat
     3181 taccgtacag atgcaaaaca aaagtggtta cttctgactg gtatatctgc acagcaaaat
     3241 cgtgtggtgg gagctatgca gctatattct gtagatagga aagtgtctca gcccattgaa
     3301 ggacatgcag ctagctttgc acagtttaag atggaaggaa atgcagaaga atcaacgtta
     3361 ttttgttttg cagttcgggg ccaagctgga gggaagttac atattattga agttggcaca
     3421 ccacctacag ggaaccagcc ctttccaaag aaggcagtgg atgtcttctt tcctccagaa
     3481 gcacaaaatg attttcctgt tgcaatgcag atcagtgaaa agcatgatgt ggtgttcttg
     3541 ataaccaagt atggttatat ccacctctat gatcttgaga ctggtacctg catctacatg
     3601 aatagaatca gtggagaaac aatttttgtt actgcacctc atgaagccac agctggaata
     3661 attggagtaa acagaaaggg acaagttctg tcagtgtgtg tggaagaaga aaacataatt
     3721 ccttacatca ccaatgttct acaaaatcct gatttggctc tgagaatggc tgtacgtaat
     3781 aacttagccg gtgctgaaga actctttgcc cggaaattta atgctctttt tgcccaggga
     3841 aattactcgg aggcagcaaa ggtggctgct aatgcaccaa agggaattct tcgtactcca
     3901 gacactatcc gtcggttcca gagtgtccca gcccagccag gtcaaacttc tcctctactt
     3961 cagtactttg gtatcctttt ggaccaggga cagctcaaca aatacgaatc cttagagctt
     4021 tgtaggcctg tacttcagca agggcgaaaa cagcttttgg agaaatggtt aaaagaagat
     4081 aagctggaat gttctgaaga actgggtgat cttgtgaaat ctgtggaccc tacattggca
     4141 cttagtgtgt acctaagggc taacgtccca aataaagtca ttcagtgctt tgcagaaaca
     4201 ggtcaagtcc aaaagattgt tttatatgct aaaaaagttg gatacactcc agattggata
     4261 tttctgctga gaaatgtaat gcgaatcagt ccagatcagg gacagcagtt tgcccaaatg
     4321 ttagttcaag atgaagagcc tcttgctgac atcacacaga ttgtagatgt ctttatggaa
     4381 tacaatctaa ttcagcagtg tactgcattc ttgcttgatg ctctgaagaa taatcgccca
     4441 tctgaaggtc ctttacagac gcggttactt gagatgaacc ttatgcatgc gcctcaagtt
     4501 gcagatgcta ttctaggcaa tcagatgttc acacattatg accgggctca tattgctcaa
     4561 ctgtgtgaaa aggctggcct actgcagcgt gcattagaac atttcactga tttatatgat
     4621 ataaaacgtg cagtggttca cacccatctt cttaaccctg agtggttagt caactacttt
     4681 ggttccttat cagtagaaga ctccctagaa tgtctcagag ccatgctgtc tgccaacatc
     4741 cgtcagaatc tgcagatttg tgttcaggtg gcttctaaat atcatgaaca actgtcaact
     4801 cagtctctga ttgaactttt tgaatctttc aagagttttg aaggtctctt ttattttctg
     4861 ggatccattg ttaactttag ccaggaccca gatgtgcact ttaaatatat tcaggcagct
     4921 tgcaagactg ggcaaatcaa agaagtagaa agaatctgta gagaaagcaa ctgctacgat
     4981 cctgagcgag tcaagaattt tcttaaggaa gcaaaactaa cagatcagct accacttatc
     5041 attgtgtgtg atcgatttga ctttgtccat gatttggtgc tctatttata tagaaataat
     5101 cttcaaaagt atatagagat atatgtacag aaggtgaatc caagtcgact tcctgtagtt
     5161 attggaggat tacttgatgt tgactgttct gaagatgtca taaaaaactt gattcttgtt
     5221 gtaagaggtc aattctctac tgatgagctt gttgctgagg ttgaaaaaag aaacagattg
     5281 aaactgcttc tgccttggct agaggccaga attcatgagg gctgtgagga gcctgctact
     5341 cacaatgcct tagccaaaat ctacatagac agtaataaca acccggagag atttcttcgt
     5401 gaaaatccct actatgacag tcgcgttgtt ggaaagtatt gtgagaagag agatccacat
     5461 ctggcctgtg ttgcttatga acgtggccaa tgtgatctgg aacttattaa tgtttgcaat
     5521 gagaattccc tcttcaaaag tctttctcgc tacctggtac gtcgaaagga tccagaattg
     5581 tggggcagcg tgctgctgga aagcaatcct tacaggagac ccctaattga ccaggttgta
     5641 caaacagctt tgtctgagac tcaggaccct gaagaagtgt cagtaactgt aaaggctttc
     5701 atgactgcag accttcctaa tgaactcatt gaactgctgg agaaaattgt ccttgataac
     5761 tctgtattca gtgaacacag gaatctgcaa aacctcctta tcctcactgc aattaaggct
     5821 gaccgtacac gtgttatgga gtatattaac cgcctggata attatgatgc cccagatatt
     5881 gccaatatcg ccatcagcaa tgagctgttt gaagaagcat ttgccatttt ccggaaagcg
     5941 gccgcagtcg accaccatca ccatcaccat taaaagcttg gcactggccg tcgttttaca
     6001 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6061 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6121 cagcctgaat ggcgaatggc agcttggctg ttttggcgga tgagataaga ttttcagcct
     6181 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
     6241 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
     6301 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
     6361 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     6421 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     6481 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
     6541 cggatggcct ttttgcgttt ctacaaactc tttttgttta tttttctaaa tacattcaaa
     6601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6841 ccccgaagaa cgttctccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6901 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7561 tgatttaccc cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat
     7621 atttaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7681 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7741 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     7801 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     7861 cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     7921 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     7981 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8041 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat
     8101 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8161 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8221 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8281 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8341 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8401 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8461 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8521 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8581 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8641 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8701 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8761 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8821 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8881 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8941 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     9001 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9061 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     9121 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     9181 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     9241 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct
     9301 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     9361 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     9421 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     9481 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     9541 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     9601 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     9661 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     9721 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     9781 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     9841 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgaa att
//
