LOCUS       sequence-189394-        9889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label:  pLgw EcoDam-V5-ELK4
FEATURES             Location/Qualifiers
     source          1..9889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2601..2839
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2786..2807
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3734..3775
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3791..3815
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5136..5160)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             5691..5871
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5897..5914)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5903..6127
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6173..6601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6260..6279)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6470..6491
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6610..6630)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6615..6944
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6795..6930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6857..6876
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6992..7039
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7058..7432
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7562..7683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7599..7618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7653..7672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7732..7748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7732..7748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7745..7767)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7756..7772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7780..7786,7787..7804,7805..7810))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7825..7846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7963..7980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8134..8722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8214..8233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8893..9684,9685..9753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9516..9535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9754..9858)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcagat ctcggccgca tattaagtgc attgttctcg ataccgctaa gtgcattgtt
     2461 ctcgttagct cgatggacaa gtgcattgtt ctcttgctga aagctcgatg gacaagtgca
     2521 ttgttctctt gctgaaagct cgatggacaa gtgcattgtt ctcttgctga aagctcagta
     2581 cccgggagta ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     2641 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     2701 gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag
     2761 taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga
     2821 atacaagaag agaactctga atactttcaa caagttaccg agaaagaaga actcacacac
     2881 agctagcgcc accatgaaga aaaatcgcgc ttttttgaag tgggcagggg gcaagtatcc
     2941 cctgcttgat gatattaaac ggcatttgcc caagggcgaa tgtctggttg agccttttgt
     3001 aggtgccggg tcggtgtttc tcaacaccga cttttctcgt tatatccttg ccgatatcaa
     3061 tagcgacctg atcagtctct ataacattgt gaagatgcgt actgatgagt acgtacaggc
     3121 cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag gtttactatc agttccgcga
     3181 agagttcaac aaaagccagg atccgttccg tcgggcggta ctgtttttat atttgaaccg
     3241 ctacggttac aacggcctgt gtcgttacaa tctgcgcggt gagtttaacg tgccgttcgg
     3301 ccgctacaaa aaaccctatt tcccggaagc agagttgtat cacttcgctg aaaaagcgca
     3361 gaatgccttt ttctattgtg agtcttacgc cgatagcatg gcgcgcgcag atgatgcatc
     3421 cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg accgccaact ttacggcgta
     3481 tcacacaaac agttttacgc ttgaacaaca agcgcatctg gcggagatcg ccgaaggtct
     3541 ggttgagcgc catattccag tgctgatctc caatcacgat acgatgttaa cgcgtgagtg
     3601 gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc agtataagca gcaacggcgg
     3661 cacacgtaaa aaggtggacg aactgctggc tttgtacaaa ccaggagtcg tttcacccgc
     3721 gaaaaaaacc ggtggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgatcga
     3781 tttcgatcaa acaagtttgt acaaaaaagc aggctccgaa ttcgccctta tggacagtgc
     3841 catcactctg tggcagttcc ttctccagct cctgcaggag cctcagaatg agcacatgat
     3901 ctgctggaca tctaacaatg gggagttcaa gcttctgcag gcggaggagg tggctcgtct
     3961 ttgggggatt cgcaagaaca agcctaatat gaattatgat aaactcagcc gagccctgcg
     4021 atactactac gtaaagaaca tcattaaaaa agtgaatggt cagaagtttg tgtacaagtt
     4081 tgtctcctat ccagagattt tgaaaatgga cccactgacg gtaggcagga ttgagggaga
     4141 ctgtgaagct ttaaattcca ttgaaaccag cagttccaaa gatgttgagt atggaggaaa
     4201 ggagaggcca cctcagcctg gagccaagac ctctagtcgc aatgactaca tacactctgg
     4261 tttgtattct tcatttactc ttaactctct gaacacctcc aataagaagc ttttcaagtc
     4321 aataaagata gagaatccag cggaaaaact agcagagaag aaagcccagg agccaacacc
     4381 gtccgtcatc aaatttgtga caacgcctgc caaaaagccg cccatcgagc ctgtcgctgc
     4441 tgcctttgca acgagcccta gtctttctcc atcttctgaa gaaactatcc aagctttgga
     4501 gacattagtc tctcccacac taccttccct ggaaacccca gcctccatat ccattctggc
     4561 taccaccttt aaccccactc ctccggttcc atccacaccc cttcctctaa aggagcctcc
     4621 caggacacct tctccaccac tgagttctaa cccagacatt gacacagaca ttgagtcagt
     4681 ggcttctcag ccaatggaac ttccagagaa cttatcactg gagcctaaaa atgaggattc
     4741 agctttgcca gaaaaggaca aaacaaataa ctcatcaaga tctaagaagc ccaaagggtt
     4801 agaactgaca cccgcccttg tggtcacagg cagtgacccc agccccttgg ggatcctaag
     4861 cccatctctc ccgacagcgt ctcttacacc agcactcttt tcacagactc ccatcttgct
     4921 gactccgagc cccttgctct ccagcatcca cttttggagc actctcagcc cgtttgctcc
     4981 cttgagccca gccagactgc aaggtgctaa tacgcttttc cagtttccct ctgtactcaa
     5041 cagtcatggc ccgttcactc tgtcaggcct ggacggacct tccactccgg gcccattttc
     5101 tccagacctg cagaagacat aaaagggcga attcgaccca gctttcttgt acaaagtggt
     5161 tcgatcgaac cgcggtctag actcgagacc tagaaaaaca tggagcaatc acaagtagca
     5221 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     5281 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5341 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5401 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     5461 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     5521 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     5581 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     5641 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     5701 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5761 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5821 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     5881 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5941 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6001 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6061 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6121 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6181 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6241 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6301 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6361 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6421 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6481 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6541 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6601 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6661 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6721 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6781 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6841 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6901 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6961 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     7021 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     7081 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     7141 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     7201 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     7261 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     7321 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     7381 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     7441 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7501 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7561 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7621 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7681 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7741 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7801 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7861 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7921 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7981 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8041 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8101 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8161 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8221 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8281 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     8341 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8401 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8461 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8521 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8581 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8641 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8701 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8761 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8821 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8881 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8941 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9001 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9061 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9121 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9181 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9241 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9301 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9361 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9421 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9481 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9541 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9601 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9661 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9721 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9781 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9841 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
