LOCUS       sequence-189400-        9876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 9876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label:  pLgw ELK4-V5-EcoDam
FEATURES             Location/Qualifiers
     source          1..9876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      266..694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(353..372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     563..584
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(703..723)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        708..1037
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      888..1023
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     950..969
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1085..1132
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1151..1525
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1655..1776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1692..1711)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1746..1765
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1825..1841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1825..1841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1838..1860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1849..1865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1873..1879,1880..1897,1898..1903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1918..1939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2056..2073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2227..2815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2307..2326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2986..3777,3778..3846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3609..3628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3847..3951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4026..4045)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4217..4596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4598..4796
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4751..4771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4814..4994
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5041..5166
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5659..5892
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6077..6121
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6583..6821
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6768..6789
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6874..6898
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8219..8243)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8254..8295
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     LTR             9660..9840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(join(9866..9876,1..7))
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(9872..9876,1..220)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
       61 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
      121 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
      181 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
      241 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
      301 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      361 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      421 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      481 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      541 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
      601 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
      661 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
      721 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      781 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      841 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      901 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
      961 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1021 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     1081 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     1141 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     1201 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     1261 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     1321 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     1381 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     1441 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     1501 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     1561 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     1621 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     1681 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1741 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     1801 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1861 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1921 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1981 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2041 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2101 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2161 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2221 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2281 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2341 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2401 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2461 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2521 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2581 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2641 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2701 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2761 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2821 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2881 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2941 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3001 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3061 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3121 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3181 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3241 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3301 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3361 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3421 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3481 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3541 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3601 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3661 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3721 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3781 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3841 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3901 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3961 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     4021 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     4081 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     4141 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     4201 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     4261 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     4321 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     4381 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     4441 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     4501 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     4561 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     4621 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     4681 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     4741 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     4801 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4861 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4921 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4981 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     5041 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     5101 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     5161 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     5221 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     5281 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     5341 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     5401 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     5461 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     5521 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     5581 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     5641 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     5701 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     5761 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     5821 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     5881 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     5941 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     6001 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     6061 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     6121 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     6181 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     6241 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     6301 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     6361 gaacggatcc cccgggctgc aggaattcag atctcggccg catattaagt gcattgttct
     6421 cgataccgct aagtgcattg ttctcgttag ctcgatggac aagtgcattg ttctcttgct
     6481 gaaagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg
     6541 ttctcttgct gaaagctcag tacccgggag taccctcgac cgccggagta taaatagagg
     6601 cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg
     6661 aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa
     6721 gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa
     6781 tctgccaaga agtaattatt gaatacaaga agagaactct gaatactttc aacaagttac
     6841 cgagaaagaa gaactcacac acagctagat caaacaagtt tgtacaaaaa agcaggctcc
     6901 gaattcgccc ttaccatgga cagtgccatc actctgtggc agttccttct ccagctcctg
     6961 caggagcctc agaatgagca catgatctgc tggacatcta acaatgggga gttcaagctt
     7021 ctgcaggcgg aggaggtggc tcgtctttgg gggattcgca agaacaagcc taatatgaat
     7081 tatgataaac tcagccgagc cctgcgatac tactacgtaa agaacatcat taaaaaagtg
     7141 aatggtcaga agtttgtgta caagtttgtc tcctatccag agattttgaa aatggaccca
     7201 ctgacggtag gcaggattga gggagactgt gaagctttaa attccattga aaccagcagt
     7261 tccaaagatg ttgagtatgg aggaaaggag aggccacctc agcctggagc caagacctct
     7321 agtcgcaatg actacataca ctctggtttg tattcttcat ttactcttaa ctctctgaac
     7381 acctccaata agaagctttt caagtcaata aagatagaga atccagcgga aaaactagca
     7441 gagaagaaag cccaggagcc aacaccgtcc gtcatcaaat ttgtgacaac gcctgccaaa
     7501 aagccgccca tcgagcctgt cgctgctgcc tttgcaacga gccctagtct ttctccatct
     7561 tctgaagaaa ctatccaagc tttggagaca ttagtctctc ccacactacc ttccctggaa
     7621 accccagcct ccatatccat tctggctacc acctttaacc ccactcctcc ggttccatcc
     7681 acaccccttc ctctaaagga gcctcccagg acaccttctc caccactgag ttctaaccca
     7741 gacattgaca cagacattga gtcagtggct tctcagccaa tggaacttcc agagaactta
     7801 tcactggagc ctaaaaatga ggattcagct ttgccagaaa aggacaaaac aaataactca
     7861 tcaagatcta agaagcccaa agggttagaa ctgacacccg cccttgtggt cacaggcagt
     7921 gaccccagcc ccttggggat cctaagccca tctctcccga cagcgtctct tacaccagca
     7981 ctcttttcac agactcccat cttgctgact ccgagcccct tgctctccag catccacttt
     8041 tggagcactc tcagcccgtt tgctcccttg agcccagcca gactgcaagg tgctaatacg
     8101 cttttccagt ttccctctgt actcaacagt catggcccgt tcactctgtc aggcctggac
     8161 ggaccttcca ctccgggccc attttctcca gacctgcaga agacaaaggg cgaattcgac
     8221 ccagctttct tgtacaaagt ggttcgatcc ggtggtaagc ctatccctaa ccctctcctc
     8281 ggtctcgatt ctacgatcga tttcgaaccg cggatgaaga aaaatcgcgc ttttttgaag
     8341 tgggcagggg gcaagtatcc cctgcttgat gatattaaac ggcatttgcc caagggcgaa
     8401 tgtctggttg agccttttgt aggtgccggg tcggtgtttc tcaacaccga cttttctcgt
     8461 tatatccttg ccgatatcaa tagcgacctg atcagtctct ataacattgt gaagatgcgt
     8521 actgatgagt acgtacaggc cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag
     8581 gtttactatc agttccgcga agagttcaac aaaagccagg atccgttccg tcgggcggta
     8641 ctgtttttat atttgaaccg ctacggttac aacggcctgt gtcgttacaa tctgcgcggt
     8701 gagtttaacg tgccgttcgg ccgctacaaa aaaccctatt tcccggaagc agagttgtat
     8761 cacttcgctg aaaaagcgca gaatgccttt ttctattgtg agtcttacgc cgatagcatg
     8821 gcgcgcgcag atgatgcatc cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg
     8881 accgccaact ttacggcgta tcacacaaac agttttacgc ttgaacaaca agcgcatctg
     8941 gcggagatcg ccgaaggtct ggttgagcgc catattccag tgctgatctc caatcacgat
     9001 acgatgttaa cgcgtgagtg gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc
     9061 agtataagca gcaacggcgg cacacgtaaa aaggtggacg aactgctggc tttgtacaaa
     9121 ccaggagtcg tttcacccgc gaaaaaataa ctcgagacct agaaaaacat ggagcaatca
     9181 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     9241 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     9301 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     9361 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     9421 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     9481 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     9541 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     9601 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     9661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9841 gggcccgttt aaacccgctg atcagcctcg actgtg
//
