LOCUS       sequence-189405-        9886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 
            using Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  TITLE     Harvey lab plasmids
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label:  pLgw EcoDam-V5-ELK1Y158A
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2601..2839
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2786..2807
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3734..3775
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3791..3815
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5133..5157)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             5688..5868
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5894..5911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5900..6124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6170..6598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6257..6276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6467..6488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6607..6627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6612..6941
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6792..6927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6854..6873
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6989..7036
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7055..7429
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7559..7680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7596..7615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7650..7669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7729..7745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7729..7745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7742..7764)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7753..7769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7777..7783,7784..7801,7802..7807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7822..7843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7960..7977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8131..8719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8211..8230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8890..9681,9682..9750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9513..9532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9751..9855)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcagat ctcggccgca tattaagtgc attgttctcg ataccgctaa gtgcattgtt
     2461 ctcgttagct cgatggacaa gtgcattgtt ctcttgctga aagctcgatg gacaagtgca
     2521 ttgttctctt gctgaaagct cgatggacaa gtgcattgtt ctcttgctga aagctcagta
     2581 cccgggagta ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     2641 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     2701 gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag
     2761 taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga
     2821 atacaagaag agaactctga atactttcaa caagttaccg agaaagaaga actcacacac
     2881 agctagcgcc accatgaaga aaaatcgcgc ttttttgaag tgggcagggg gcaagtatcc
     2941 cctgcttgat gatattaaac ggcatttgcc caagggcgaa tgtctggttg agccttttgt
     3001 aggtgccggg tcggtgtttc tcaacaccga cttttctcgt tatatccttg ccgatatcaa
     3061 tagcgacctg atcagtctct ataacattgt gaagatgcgt actgatgagt acgtacaggc
     3121 cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag gtttactatc agttccgcga
     3181 agagttcaac aaaagccagg atccgttccg tcgggcggta ctgtttttat atttgaaccg
     3241 ctacggttac aacggcctgt gtcgttacaa tctgcgcggt gagtttaacg tgccgttcgg
     3301 ccgctacaaa aaaccctatt tcccggaagc agagttgtat cacttcgctg aaaaagcgca
     3361 gaatgccttt ttctattgtg agtcttacgc cgatagcatg gcgcgcgcag atgatgcatc
     3421 cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg accgccaact ttacggcgta
     3481 tcacacaaac agttttacgc ttgaacaaca agcgcatctg gcggagatcg ccgaaggtct
     3541 ggttgagcgc catattccag tgctgatctc caatcacgat acgatgttaa cgcgtgagtg
     3601 gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc agtataagca gcaacggcgg
     3661 cacacgtaaa aaggtggacg aactgctggc tttgtacaaa ccaggagtcg tttcacccgc
     3721 gaaaaaaacc ggtggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgatcga
     3781 tttcgatcaa acaagtttgt acaaaaaagc aggctccgaa ttcgccctta tggacccatc
     3841 tgtgacgctg tggcagtttc tgctgcagct tctgagagaa caaggtaatg gccacatcat
     3901 ctcctggacc tcacgggatg gtggtgagtt caagttggtg gatgcagagg aggtggcccg
     3961 gctatgggga ctgcgcaaga acaagaccaa catgaattac gacaagctta gccgggcctt
     4021 gcggtactac tatgataaga atatcatccg caaggtgagc ggccagaagt ttgtctacaa
     4081 gtttgtgtcc tacccagagg ttgcagggtg ctccactgaa gactgcccac cccagcctga
     4141 ggtgtctgta acctcggcca tagccatggc ccctgctact gtccatgcag gcccagggga
     4201 cactgccact ggaaagccag gaacaccaaa gggtgcagga atgacaggcc aaggtggctt
     4261 agcacgaagc agccggaatg aatacatgcg ctcgggcctc gcgtctacct tcacaataca
     4321 atccctgcag ccacagccac agccacccat tcctcctcgg cctgcctcag tgcttcccaa
     4381 cactacccct gcaggagtac cagcacccgc ctcagggagc aggagcacca gtccaaaccc
     4441 cttagaagcc tgtttggaag cagaagaggc tggtctgccc ctgcaggtta tcctaacccc
     4501 accagaggcc ccaaaccaga aatccgaaga gttgagtctg gacccaagtt ttggccatcc
     4561 acagccccca gaagtcaaag tggaggggcc taaggaagaa ttggaagctg caagggctgg
     4621 aggcttcagt tcagaagctg tcaaagctga accagaagtc tcagcctcag aaggcctgct
     4681 ggctcggctc ccagccatcc taacagagaa cacagcccag gtgtgtggcc tctccacttc
     4741 caccactgag atcacccaac cgcagaaggg ccgaaagcct cgggacctgg aacttccact
     4801 tagcccaagc ctgctgggtg gccagggacc tgaacggact ccaggatcag gaacaagctc
     4861 tggtcttcag gcaccggggc cagcgctaac gccatccctg ctccccacac ataccttgac
     4921 cccggtgctg ctgacaccca gctcgctgcc ccctagcatc catttctgga gcactctgag
     4981 tccaattgca ccccgtagtc cagccaagct ctccttccag tttccgtcca gtggcagcgc
     5041 acaggtgcac atcccttcca tcagtgtgga tggcctctcg acccccgtgg tgctctcccc
     5101 agggccccag aagccatgaa agggcgaatt cgacccagct ttcttgtaca aagtggttcg
     5161 atcgaaccgc ggtctagact cgagacctag aaaaacatgg agcaatcaca agtagcaata
     5221 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     5281 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     5341 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5401 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     5461 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     5521 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     5581 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     5641 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     5701 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5761 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5821 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     5881 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5941 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6001 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6061 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6121 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     6181 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6241 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6301 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6361 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6421 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6481 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6541 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6601 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6661 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6841 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     6961 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     7021 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     7081 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     7141 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     7201 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     7261 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     7321 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     7381 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     7441 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7501 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7681 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7741 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7801 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7861 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7921 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8041 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8761 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8821 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8881 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9001 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9061 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9181 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9541 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9721 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9781 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9841 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
