LOCUS       sequence-189413-        3151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for NKX2-5 YRD Y-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pENTR-2B-Nkx2-5YRD Y-A
FEATURES             Location/Qualifiers
     source          1..3151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(722..821)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(840..863)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(871..898)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(990..1076)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1241..1829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1321..1340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1919..2728)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2642..2661
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2720..2739
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2851..2950
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gggtccccca ggcagggctt cccgtcgcgc accagcacgg gcaccgcgat cctgcgcgcg
       61 ggcggcggcg gcggccccag aagctccaga gtctggtcct gccgctgtcg cttgcacttg
      121 tagcgacggt tctggaacca gatcttgacc tgcgtggacg tgagcttcag cacgctggcc
      181 agctggtcgc gctctggcgc cgacaggtac cgctgttgct tgaagcgccg ctccagctcg
      241 tagacctgcg cctgcgagaa gagcacgcgt ggcttccgtc gccgccgtgc gcgtggtctc
      301 tcggcgccat ccgtctcggc tttgtccagc tccactgcct tctgcagcgc gcacagctct
      361 tttttatccg cccgagggtc tttggctggg tcagggtcac cgtaggctcc cgggtaaaat
      421 gtgggggcgg ctgggaaagc aggagagcac ttggggggcg aaggcgcggg gcccatctcc
      481 gcgcgcagct ctgccaggcc ggacgctgcc gcctcggggc cagagtaggc ctcgggcttg
      541 aaggcggcca gcatgcagga ggcaggggcc agggtggcct cgaggcgcgc agacaggtcc
      601 ccagacgcca ggctacgctg ctgctgctcc aggttcagga tgtctttgac tgagaaaggc
      661 gtgggtgtga gcgcagggct ggggaacatc ggatccagtc gactgaattg gttccggcgc
      721 cagcctgctt ttttgtacaa agttggcatt ataaaaaagc attgcttatc aatttgttgc
      781 aacgaacagg tcactatcag tcaaaataaa atcattattt ggggcccgag cttaagtaac
      841 taactaacag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      901 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      961 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     1021 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
     1081 ctggcagttc cctactctcg cttagtagtt agacgtcccc gagatccatg ctagcggtaa
     1141 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1201 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1261 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1321 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1381 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1441 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1501 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1561 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1681 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1741 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1801 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1861 acgctcagtg gaacggggcc caatctgaat aatgttacaa ccaattaacc aattctgatt
     1921 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     1981 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     2041 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     2101 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     2161 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     2221 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     2281 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     2341 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     2401 cttctaatac ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac catgcatcat
     2461 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     2521 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     2581 actctggcgc atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat
     2641 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     2701 tcgacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     2761 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     2821 gagacacggg ccagagctgc agctggatgg caaataatga ttttattttg actgatagtg
     2881 acctgttcgt tgcaacaaat tgataagcaa tgctttctta taatgccaac tttgtacaag
     2941 aaagctgggt ctagatatct cgagtgcggc cgctaccagg ctcggatgcc gtgcagcgtg
     3001 gagacgcccg aattgccctg cggcatcccg ggactctgca cggtgttcaa gtccccgacg
     3061 ccaaagttca cgaagttgct gttggcggag gcggcggggg gctgcgcggc ggggggcgca
     3121 gcggggtagg cggcgcagct gtagccggga c
//
