LOCUS       sequence-18942-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     Induction of cre recombinase activity using modified androgen 
            receptor ligand binding domains: a sensitive assay for 
            ligand-receptor interactions.
  JOURNAL   Nucleic Acids Res. 2003 Aug 1. 31(15):e86.
  PUBMED    12888538
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Aug 1. 31(15):e86."
COMMENT     Sequence Label: p274 pCMV-CRE-M-AR(T)
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    132..165
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     intron          387..519
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgactctaga ggatcccctc gagcggccgc ctaggccatg gcgaatttac
      121 tgacggtacc aataacttcg tataatgtat actatacgaa gttattcgaa ttccaaaatt
      181 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
      241 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
      301 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
      361 ttcgcgatta tcttctatat ctgcaggtaa gtatcaaggt tacaagacag gtttaaggag
      421 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
      481 ttggtcttac tgacatccac tttgcctttc tctccacagg cgcgcggtct ggcagtaaaa
      541 actatccagc aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga
      601 ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat
      661 gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt
      721 tcactcatgg aaaatagcga tcgctgccag gatatac
//
