LOCUS       sequence-189424-        4346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of NS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Huang X, Zheng M, Wang P, Mok BW, Liu S, Lau SY, Chen P, Liu YC, Liu
            H, Chen Y, Song W, Yuen KY, Chen H
  TITLE     An NS-segment exonic splicing enhancer regulates influenza A virus 
            replication in mammalian cells.
  JOURNAL   Nat Commun. 2017 Mar 21;8:14751. doi: 10.1038/ncomms14751.
  PUBMED    28323816
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-03-21"; volume: "8:14751. doi"; pages: " 
            10.1038/ncomms14751"
COMMENT     Sequence Label: pCX-V5-H7N9-NS1
FEATURES             Location/Qualifiers
     source          1..4346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..61
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     123..142
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             838..879
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(983..1002)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1048..1103
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1102..1121)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1464..1480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1464..1480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1477..1499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1488..1504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1512..1518,1519..1536,1537..1542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1557..1578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1637..1832
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1683..1818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1838..1972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1875..1894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1929..1948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2040..2057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2211..2799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2291..2310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2970..3761,3762..3830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3593..3612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3831..3935)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3966..4345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
       61 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
      121 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg cggccgcatg
      181 gattccaata ctgtgtcaag cttccaggta gactgctttc tttggcatgt ccgcaaacga
      241 tttgcagacc aagaaatggg tgatgcccca tttctagacc ggcttcgccg agatcagaag
      301 tccctgagag gaagaagcag cactcttggt ctggacatca gaactgccac gcgtgaagga
      361 aagcatatag tggagcggat tttagaggaa gagtcagatg aagcatttaa aatgagtatt
      421 gcttcagtgc cagctccacg ctatctaact gacatgactc ttgaagaaat gtcaagagat
      481 tggttaatgc tcattcccaa acagaaaata acagggtccc tatgcattag aatggaccaa
      541 gcaatagtgg acaaaaacat cacattgaaa gcaaatttca gtgtgatttt caatcggctt
      601 gaagccctga tactacttag agcttttacg gaagaaggag caattgtagg cgaaatctca
      661 ccattacctt ctcttccagg acatactgac aaggatgtca aaaatgcaat tgagatcctc
      721 atcggaggat ttgaatggaa tgataacaca gttcgagtct ctgaaactct acagagattc
      781 gcttggagaa gcagcgatga ggatgggaga tctccactct ctacaaagct cgagatgggt
      841 aagcctatcc ctaaccctct cctcggtctc gattctacgt aggtaccatc gataccggtg
      901 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
      961 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     1021 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     1081 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     1141 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     1201 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     1261 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     1321 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     1381 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     1441 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1501 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1561 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1621 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     1681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     1741 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     1801 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     1861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     1981 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2041 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2101 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2401 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2641 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2881 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2941 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3001 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3061 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3121 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3181 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3241 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3301 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3361 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3421 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3481 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3541 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3601 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3661 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3721 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3781 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3841 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3901 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3961 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4021 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4081 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4141 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4201 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4261 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4321 cgtattagtc atcgctatta ccatgg
//
