LOCUS       sequence-18945-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Dantas G, Kuhlman B, Callender D, Wong M, Baker D
  TITLE     A large scale test of computational protein design: folding and 
            stability of nine completely redesigned globular proteins.
  JOURNAL   J Mol Biol. 2003 Sep 12. 332(2):449-60.
  PUBMED    12948494
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2003 Sep 12. 332(2):449-60."
COMMENT     Sequence Label: FKB
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(379..423)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             434..456
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    471..495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(495..514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(496..514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(602..621)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgagagaa ccaacaagct ggactaataa
       61 aacatcgaaa attaatacag tgcccggcgg aaccacaccc ggtaaacccg tttcaccata
      121 cgttttactg ctgtcgatta caattgttac gcgttcgccc tgggataagt ttgcgaccgc
      181 ttcctgcagg ccctccggaa cttggttttg gcccagataa aagcgatacg gagtgccctg
      241 atcggcagat gaagaaattg gtgggccatc cttatgcatc cacgtataga agataatgac
      301 taattcgcca ggttttggac gattgttccc atcgccactt tcctgcgtaa ctacggtaac
      361 accggaaccc agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc
      421 tttcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      481 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg
      541 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      601 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      661 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      721 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      781 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg
      841 gcgcaaaacc tttcgcggta tggcatgata gcgc
//
