LOCUS       sequence-189457-        1397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral tet-inducible expression of mito-targeted genetically 
            encoded Ca2+-indicators for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Mughal W, Martens M, Field J, Chapman D, Huang J, Rattan S, Hai Y, 
            Cheung KG, Kereliuk S, West AR, Cole LK, Hatch GM, Diehl-Jones W, 
            Keijzer R, Dolinsky VW, Dixon IM, Parmacek MS, Gordon JW
  TITLE     Myocardin regulates mitochondrial calcium homeostasis and prevents 
            permeability transition.
  JOURNAL   Cell Death Differ. 2018 Mar 6. pii: 10.1038/s41418-018-0073-z. doi: 
            10.1038/s41418-018-0073-z.
  PUBMED    29511336
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2018 Mar 6. pii: 10.1038/s41418-018-0073-z. doi: 
            10.1038/s41418-018-0073-z."
COMMENT     Sequence Label: pLenti-Mito-Car-GECO
FEATURES             Location/Qualifiers
     source          1..1397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..138
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             187..243
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     939..958
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctcacaggtt ccgcaagaag actccccgtg cctagggcca aaattcattc actgggggac
       61 cccatgagcg tgctcacccc actcctgctg cgggggctga ccggcagcgc taggcggctg
      121 ccagtcccca gggccaagat ccacagtctc ggcgatccca aggatccaac catggtcgac
      181 tcttcacgtc gtaagtggaa taaggcaggt cacgcagtta gagctatagg tcggctgagc
      241 tcacccgtgg tttccgagcg gatgtacccc gaggacggcg ccctgaagag cgagatcaag
      301 aaggggctga ggctgaagga cggcggccac tacgccgccg aggtcaagac cacctacaag
      361 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcgtg
      421 tcccacaacg aggactacac catcgtggaa cagtgcgaac gcgccgaggg ccgccactcc
      481 accggcggca tggacgagct gtacaaggga ggtacaggcg ggagtctggt gagcaagggc
      541 gtggaggata acatggccat cgtcaaggag ttcatgcgct tcaagacgca catagagggc
      601 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc
      661 tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      721 ctgtcccctc agattatgta cggctccaag gcctacatta agcacccagc cgacatcccc
      781 gactacttca agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag
      841 gacggcggca ttattcacgt taaccaggac tcctccctgc aggacggcgt attcatctac
      901 aaggtgaagc tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
      961 atgggctggg agcctacgcg tgaccaactg actgaagagc agatcgcaga atttaaagag
     1021 gctttctccc tatttgacaa ggacggggat gggacgataa caaccaagga gctggggacg
     1081 gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat gatcaatgaa
     1141 gtagatgccg acggtgacgg cacattcgac ttccctgagt tcctgacgat gatggcaaga
     1201 aaaatgaatg acacagacag tgaagaggaa attagagaag cgttccgcgt gtttgataag
     1261 gacggcaatg gctacatcgg cgcagcagag cttcgccacg tgatgacaga ccttggagag
     1321 aagttaacag atgaggaggt tgatgaaatg atcagggtag cagacatcga tggggatggt
     1381 caggtaaact acgaaga
//
