LOCUS       sequence-18946-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  TITLE     Top7 plasmids
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Top7 D9T D10V N14T
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(358..375)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             386..408
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    423..447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(447..466)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(448..466)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(554..573)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctccttcggg cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtcatta
       61 gtcgacgccc tctaactgtc cttccactgt caccgtatcg ccgtcccacg taacgttaat
      121 atcattataa cccagttcgg cgaaaacttt aatcaaaatc gcggcaaatt tttctgcttc
      181 ttttttcgta cgagcggtaa tcgagatgcg aacgcgttta gccccctgtt ttttgatgta
      241 gtctttcagt tcgttcaaaa ccttctgtaa ttccgattct gtggtcacgg tataagtata
      301 atcgaaggtt ttaccattca cggtaatgtt cacttgaacc tggatatccc cagatctgtg
      361 atggtggtga tggtgcatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga
      421 ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg
      481 agatcgatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      541 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      601 gggctcatga gcgcttgttt cggcgtgggt atngcg
//
