LOCUS       sequence-189505-       11179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express rabies virus CVS N2c strain polymerase gene (N2cL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11179)
  TITLE     Genetic Neuroengineering Group Plasmids 2017
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-N2cL
FEATURES             Location/Qualifiers
     source          1..11179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3378..3389
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(5181..5192)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8203..8222)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8268..8323
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8322..8341)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8682..8698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8682..8698)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8695..8717)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8706..8722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8730..8736,8737..8754,8755..8760))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8775..8796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8855..9050
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8901..9036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9056..9190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9093..9112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9147..9166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9258..9275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9429..10017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9509..9528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10188..10979,10980..11048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10811..10830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11049..11153)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccgcc accatgctag
     1741 atccgggaga ggtttatgat gaccctattg atccaattga gtcagaggct gaacccagag
     1801 gaacccccac tgtccccaac atcttgagga actccgacta caatctcaat tctcctttga
     1861 tagaggatcc tgccaaacta atgttagaat ggttgaagac agggaacaga ccttatcgga
     1921 tgactttgac agacaattgc tccaggtctt acaaagtttt gaaagattat ttcaagaaag
     1981 tagatttggg ttctctcaaa gtgggcggaa ctgctgcaca gtcaatggtt tctctctggt
     2041 tgtgtggtgc ccactctgaa tcaaacagga gccggagatg tataaccgac ttggcccatt
     2101 tctattccaa gtcatccccc atagagaagc tattgaattg tacgctagga aacagaggcc
     2161 tgagaatccc accagagggg gtgttaaatt gcctcgagag ggtcaattat gacaaggcat
     2221 ttgggaggta tctggccaac acgtattcct cttacttgtt tttccatgta atcaccttat
     2281 acatgaatgc cttagactgg gaagaggaaa agaccatcct ggcattatgg aaagatataa
     2341 cctcagtgga taccgagaag gacttggtca aattcaaaga tcaaatatgg ggactgttga
     2401 ttgtgacaaa ggactttgtt tactctcaga gttcaaactg tctttttgac agaaactaca
     2461 cactgatgct aaaggatctt ttcttgtctc gattcaactc cttaatgatt ctgctttctc
     2521 cccctgagcc ccgatactca gatgacttaa tatctcagct gtgccagcta tacatcgctg
     2581 gggatcaagt cttgtccatg tgtgggaact ccggctatga agtcatcaaa atattggagc
     2641 catatgtcgt gaacagtttg gtccagaggg cagagaagtt taggcctctc atccacccct
     2701 tgggagactt tcctatgttc ataaaagaca aggtgaatca acttgaaggg acttttggtc
     2761 ccagtgcaaa gaggtttttt agggttttag atcaattcga caacatacat gacttagtat
     2821 ttgtgtatgg ctgttacaga cattgggggc acccctatat agattatcgg aagggtctgt
     2881 cgaaactata tgatcaagtt cacattaaga aagtaataga taagtcctac caggagtgtt
     2941 tagcaagtga cttggccaga aggatcctca gatggggatt tgacaagtac tccaaatggt
     3001 atctagattc gagattcctt gcccgagacc accccttgac tccttatgtc aagacccaaa
     3061 catggccacc caaacatata gtagacttgg tgggggacac atggcataag ctcccgatca
     3121 cgcagatctt tgaaattcct gaatcaatgg acccgtcaga gatactggat gacaaatcac
     3181 attctttcac cagaacaagg ttagcttctt ggctgtcaga gaaccgaggg gggcctgttc
     3241 ctagcgagaa ggtcattatc acggccctgt ctaagccacc tgtcaatccc cgagagtttt
     3301 tgaaatctat agacctcgga ggattgccag atgaggattt gataattggc ctcaaaccaa
     3361 aggaacggga gttgaagatc gagggccgat tctttgctct aatgtcatgg aatctaagat
     3421 tatattttgt catcaccgaa aagctcttgg ccaactacat tttgccactt tttgacgcac
     3481 tgactatgac agacaacctg aacaaggtgt tcaaaaagtt gatcgacagg gtcaccgggc
     3541 aagggctttt ggactattct agggtcacat acgcatttca cctggactat gagaaatgga
     3601 acaatcatca aagattggag tcaacagagg atgtattctc tgtcctagat caggtgtttg
     3661 gattgaagag ggtgttttct agaacacacg agttttttca gaagtcctgg atctattatt
     3721 cagacagatc agacctcatt gggttatggg aggaccaaat atattgcttg gatatgtcta
     3781 acggcccaac ctgctggaat ggccaagatg gcgggctaga gggcttgcgg cagaagggct
     3841 ggagtctagt cagtttatta atgatagata gagaatctca aaccaggaac acaagaacca
     3901 agatactagc tcaaggagac aaccaggttc tgtgtcctac atacatgttg tcaccgggat
     3961 tgtctcaaga ggggcttctc tatgagttag agagcatatc aaggaatgca ctctcaatat
     4021 accgagctat cgaggaagga gcatctaagc tggggctgat catcaagaag gaagagacca
     4081 tgtgtagtta tgactttctc atatatggga agaccccctt atttcgaggc aacatattgg
     4141 tgcctgaatc caaaagatgg gcccgagtct cttgcatctc taacgaccaa atagtcaacc
     4201 tcgccaatat aatgtcgaca gtatccacca atgcgctgac agtggcacaa cactctcaat
     4261 ctctgatcaa acctatgagg gattttctgc tcatgtcagt acaggcagtt ttccactacc
     4321 tgttgtttag cccaatccta aaaggcagag tttataagat tctgagtgct gaaggggaga
     4381 gctttctcct agccatgtcg cggataatct acctagatcc ttctttggga ggggtgtctg
     4441 gaatgtctct cgggaggttc catatacgtc agttctcaga ccctgtctct gaagggttgt
     4501 cattctggag agagatctgg ttaggctctc atgagtcctg gattcacgcg ttgtgtcagg
     4561 aggccgggaa ccccgatctt ggagagagaa cactcgagag cttcactcgc cttttagaag
     4621 atcctactac cttaaatatc aaaggagggg ccagccctac cattctactc aaggatgcta
     4681 tcagaaaggc tctgtacgac gaggtggaca aggtggaaaa ttcagagttt cgagaggcaa
     4741 tcctgctgtc caagacccat agagataact ttatactctt tttaaaatct gttgagcctc
     4801 tgttccctcg atttctcagt gagctcttca gttcgtcttt cttgggaata ccggagtcaa
     4861 tcattgaact gatacaaaac tcccggacaa taagaaggca gtttagaaag agtctctcaa
     4921 gaactttaga agagtccttc tacaactcag agatccacgg gattaatcgg ataacccaga
     4981 cacctcaaag ggtcggaagg gtgtggcctt gctcttcaga gagggcagat ctacttaggg
     5041 agatctcttg ggggaggaaa gtggtaggca cgacagttcc tcacccttcc gagatgttgg
     5101 agttgtttcc caaatcctcc atttcctgca cttgtggagc aacaggggga ggcaatccta
     5161 gagtctctgt atcagtactc ccgtccttcg atcagtcatt tttctcacgg ggccccctaa
     5221 agggatactt gggctcgtcc acctccatgt caacccagct attccatgca tgggaaaaag
     5281 tcactaatgt tcatgtggtg aaaagggctc tatcgttaaa agaatctata aactggttca
     5341 tcaataggaa ttccaatttg gctcaaactc taattggaaa catcatgtct ctgacaggcc
     5401 ctgatttccc tctagaagag gcccctgttt tcaaacggac agggtcagcc ttgcataggt
     5461 tcaagtctgc cagatacagc gaaggagggt attcttctgt ttgccctaac cttctctccc
     5521 atatctctgt tagtacagac actatgtctg atttgaccca aaacgggaag aactatgatt
     5581 tcatgtttca gccattgatg ctttatgcgc aaacatggac atcggaactg gtacagaggg
     5641 atacaagact tagagactcc acgtttcact ggcaccttcg gtgcaacaga tgtgtgaggc
     5701 ccattgatga tataacactg gaaacttctc agatcttcga gttcccggat gtgtcaaaaa
     5761 ggatatccag gatggtttct ggagctgtcc ctcaatttca gaagcttcct gatatccgtc
     5821 taagaccagg tgattttgaa tccctaagtg gtagagaaaa gtctcgccat atagggtcag
     5881 ctcaggggct cttatactca atcttagtag caattcacga ctcaggatac aatgatggga
     5941 ccatcttccc tgtcaacata tacggcaaag tttcccccag agactatttg agagggcttg
     6001 caagagggat cttgataggg tcctcgattt gcttcttgac acgaatgaca aatattaaca
     6061 ttaacagacc tcttgaattg atctcagggg taatttccta tattctcctg aggctggata
     6121 atcatccctc tctgtatata atgcttagag aaccgtctct tagaggagaa atattctcta
     6181 tccctcagaa aatccccgcc gcttacccaa ccactatgag agaaggcaac agatcgattt
     6241 tgtgttacct ccaacacgtg ctacgctatg agcgagaggc aatcacggcg tccccggaga
     6301 atgactggct gtggatcttc tcagacttca gaagtgtgaa aatgacgtac ttgaccctca
     6361 ttacctacca gtctcacctc ctactccaga gggttgagag aaacttgtct aagagtatga
     6421 gagctactct gcgacaaatg agttccttaa tgaggcaagt gctgggtggg cacggagaag
     6481 ataccttgga gtcagacgat gacattcaac gattactaaa agactctttg cgaaagacaa
     6541 ggtgggtgga tcaagaggtg cgccatgcag ctagaaccat gaatggagat tacagccccg
     6601 acaagaaagt atcccacaag gcaggatgtt cagaatgggt ctgctctgct caacagattg
     6661 ccgtctccac ctcagccaac ccggcccctg tctcagagct tgacattagg gccctctcta
     6721 agaggtttca aaaccccttg atctcgggcc tgagagtggt tcagtgggca accggtgccc
     6781 attataagct taagcctatt ctagatgatc taaatgtttt cccatctctc tgtcttgtaa
     6841 ttggagacgg gtcaggggga atatcaaggg cagttctcaa catgtttcca gattctaagc
     6901 ttgtgttcaa cagcctattg gaggtgaatg atctgatggc ttccggaaca catccactgc
     6961 ctccttcagc aatcatgagt ggaggagatg acatcatctc cagagtgata gactttgact
     7021 caatatggga gaaaccgtcc gacctgagga actcggccac ctggagatac ttccagtcgg
     7081 ttcaaaaaca ggtcaacatg tcgtatgacc tcattatttg tgatgcagaa gttactgata
     7141 ttgcatctat caaccggata actctgttga tgtctgattt cgcattgtct atagatggac
     7201 cactttatct ggtcttcaaa acttacggga ctatgctagt aaacccggac tataaagcta
     7261 ttcaacatct gtcaagagcg ttcccttcgg tcacagggtt tgtaacccaa gtaacttcat
     7321 ccttttcttc tgagctatac ctccggttct ctaaacgagg aaagtttttc agggacgccg
     7381 agtacttgac ctcttccacc cttcgagaga tgagccttgt gttgttcaat tgcagcagcc
     7441 ccaaaagtga gatgcagaga gctcgttcct taaactatca agacctggta aggggatttc
     7501 ctgaagagat catatcaaat ccttacaacg aaatgatcat aactctgatt gacaatgatg
     7561 tagagtcctt cctagtccac aagatggtgg atgatcttga gctacagagg ggaactctgt
     7621 ctaaagtggc tatcattata tccatcatga tcgttttttc caatagagtc ttcaacattt
     7681 ccaaaccttt gactgacccc ttgttctacc ccccatctga tcctaaaatc ctgaggcact
     7741 tcaacatatg ttgcagtact atgatgtatc tatctaccgc tttaggcgac gtccctaact
     7801 tcgcaagact tcatgacctg tataacagac ctataacttg ttacttcaga aaacaagtta
     7861 ttcgagggaa tatttatcta tcttggagtt ggtccgatga taccccagtg ttcaagagag
     7921 tagcctgtaa ttctagcttg agtctgtcat ctcactggat caggttgatc tacaagatag
     7981 tgaagactac cagactcatt ggcagcataa aagacctatc aggagaggta gaacgacatc
     8041 ttcatgggta taacagatgg atcaccctcg aggatatccg atctagatca tccctactag
     8101 attacagttg tttgtaagcg gccgcactcc tcaggtgcag gctgcctatc agaaggtggt
     8161 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     8221 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     8281 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     8341 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     8401 catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc
     8461 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     8521 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     8581 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     8641 gaagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8701 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8761 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8821 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     8881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9001 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     9061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9181 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9241 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9421 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9601 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     9661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9841 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11161 ccccgaaaag tgccacctg
//
