LOCUS       sequence-189513-        8439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AID-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, 
            Kim YB, Badran AH, Liu DR
  TITLE     Improved base excision repair inhibition and bacteriophage Mu Gam 
            protein yields C:G-to-T:A base editors with higher efficiency and 
            product purity.
  JOURNAL   Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. 
            eCollection 2017 Aug.
  PUBMED    28875174
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2017-08-30"; volume: "3(8):eaao4774. doi"; pages: " 
            10.1126/sciadv.aao4774. eCollection 2017 Aug"
COMMENT     Sequence Label: AID-BE3
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..1002
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     CDS             join(1051..4449,4450..4452,4453..5049,5050,5051..5053,
                     5054..5055,5056..5058,5059..5151)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1051..1074,1075..1077,1078..5151)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5164..5412
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5425..5445
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5454..5471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5494..5511)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5500..5724
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5795..5811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5795..5811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5808..5830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5819..5835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5843..5849,5850..5867,5868..5873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5888..5909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6026..6043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6197..6785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6277..6296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6956..7747,7748..7816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7579..7598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7817..7921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8000..8019)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8191..8439,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggatagcctc
      421 ttgatgaata gacgcaagtt cctgtatcag tttaaaaacg tgagatgggc aaaaggccga
      481 cgagagacat atctgtgcta tgtcgttaag cgcagagatt cagccaccag tttctctctc
      541 gacttcggct acctgcggaa caagaatggt tgccatgttg agctcctgtt cctgaggtat
      601 atcagcgact gggatttgga cccagggcgg tgctataggg tgacatggtt tacctcctgg
      661 tcaccttgtt atgactgcgc gcggcatgtt gccgattttc tgagagggaa ccctaacctg
      721 tctctgagga tcttcaccgc gcgactgtac ttctgtgagg accggaaagc cgaacccgag
      781 ggactgagac gcctccacag agcgggtgtg cagattgcca taatgacctt taaggactac
      841 ttctactgct ggaacacctt cgtcgaaaat cacgagcgga ctttcaaggc ttgggaagga
      901 ttgcatgaaa acagcgtcag gctttccagg cagcttcgcc gcattcttct cccgttgtac
      961 gaggttgatg acctcagaga tgcctttaga acactgggac tgagcggcag cgagactccc
     1021 gggacctcag agtccgccac acccgaaagt gataaaaagt attctattgg tttagccatc
     1081 ggcactaatt ccgttggatg ggctgtcata accgatgaat acaaagtacc ttcaaagaaa
     1141 tttaaggtgt tggggaacac agaccgtcat tcgattaaaa agaatcttat cggtgccctc
     1201 ctattcgata gtggcgaaac ggcagaggcg actcgcctga aacgaaccgc tcggagaagg
     1261 tatacacgtc gcaagaaccg aatatgttac ttacaagaaa tttttagcaa tgagatggcc
     1321 aaagttgacg attctttctt tcaccgtttg gaagagtcct tccttgtcga agaggacaag
     1381 aaacatgaac ggcaccccat ctttggaaac atagtagatg aggtggcata tcatgaaaag
     1441 tacccaacga tttatcacct cagaaaaaag ctagttgact caactgataa agcggacctg
     1501 aggttaatct acttggctct tgcccatatg ataaagttcc gtgggcactt tctcattgag
     1561 ggtgatctaa atccggacaa ctcggatgtc gacaaactgt tcatccagtt agtacaaacc
     1621 tataatcagt tgtttgaaga gaaccctata aatgcaagtg gcgtggatgc gaaggctatt
     1681 cttagcgccc gcctctctaa atcccgacgg ctagaaaacc tgatcgcaca attacccgga
     1741 gagaagaaaa atgggttgtt cggtaacctt atagcgctct cactaggcct gacaccaaat
     1801 tttaagtcga acttcgactt agctgaagat gccaaattgc agcttagtaa ggacacgtac
     1861 gatgacgatc tcgacaatct actggcacaa attggagatc agtatgcgga cttatttttg
     1921 gctgccaaaa accttagcga tgcaatcctc ctatctgaca tactgagagt taatactgag
     1981 attaccaagg cgccgttatc cgcttcaatg atcaaaaggt acgatgaaca tcaccaagac
     2041 ttgacacttc tcaaggccct agtccgtcag caactgcctg agaaatataa ggaaatattc
     2101 tttgatcagt cgaaaaacgg gtacgcaggt tatattgacg gcggagcgag tcaagaggaa
     2161 ttctacaagt ttatcaaacc catattagag aagatggatg ggacggaaga gttgcttgta
     2221 aaactcaatc gcgaagatct actgcgaaag cagcggactt tcgacaacgg tagcattcca
     2281 catcaaatcc acttaggcga attgcatgct atacttagaa ggcaggagga tttttatccg
     2341 ttcctcaaag acaatcgtga aaagattgag aaaatcctaa cctttcgcat accttactat
     2401 gtgggacccc tggcccgagg gaactctcgg ttcgcatgga tgacaagaaa gtccgaagaa
     2461 acgattactc catggaattt tgaggaagtt gtcgataaag gtgcgtcagc tcaatcgttc
     2521 atcgagagga tgaccaactt tgacaagaat ttaccgaacg aaaaagtatt gcctaagcac
     2581 agtttacttt acgagtattt cacagtgtac aatgaactca cgaaagttaa gtatgtcact
     2641 gagggcatgc gtaaacccgc ctttctaagc ggagaacaga agaaagcaat agtagatctg
     2701 ttattcaaga ccaaccgcaa agtgacagtt aagcaattga aagaggacta ctttaagaaa
     2761 attgaatgct tcgattctgt cgagatctcc ggggtagaag atcgatttaa tgcgtcactt
     2821 ggtacgtatc atgacctcct aaagataatt aaagataagg acttcctgga taacgaagag
     2881 aatgaagata tcttagaaga tatagtgttg actcttaccc tctttgaaga tcgggaaatg
     2941 attgaggaaa gactaaaaac atacgctcac ctgttcgacg ataaggttat gaaacagtta
     3001 aagaggcgtc gctatacggg ctggggacga ttgtcgcgga aacttatcaa cgggataaga
     3061 gacaagcaaa gtggtaaaac tattctcgat tttctaaaga gcgacggctt cgccaatagg
     3121 aactttatgc agctgatcca tgatgactct ttaaccttca aagaggatat acaaaaggca
     3181 caggtttccg gacaagggga ctcattgcac gaacatattg cgaatcttgc tggttcgcca
     3241 gccatcaaaa agggcatact ccagacagtc aaagtagtgg atgagctagt taaggtcatg
     3301 ggacgtcaca aaccggaaaa cattgtaatc gagatggcac gcgaaaatca aacgactcag
     3361 aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat taaagaactg
     3421 ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa cgagaaactt
     3481 tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact ggacataaac
     3541 cgtttatctg attacgacgt cgatcacatt gtaccccaat cctttttgaa ggacgattca
     3601 atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga caatgttcca
     3661 agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa tgcgaaactg
     3721 ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt gtctgaactt
     3781 gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac aaagcatgtt
     3841 gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa gctgattcgg
     3901 gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa
     3961 ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta tcttaatgcc
     4021 gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt tgtgtatggt
     4081 gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag
     4141 gctacagcca aatacttctt ttattctaac attatgaatt tctttaagac ggaaatcact
     4201 ctggcaaacg gagagatacg caaacgacct ttaattgaaa ccaatgggga gacaggtgaa
     4261 atcgtatggg ataagggccg ggacttcgcg acggtgagaa aagttttgtc catgccccaa
     4321 gtcaacatag taaagaaaac tgaggtgcag accggagggt tttcaaagga atcgattctt
     4381 ccaaaaagga atagtgataa gctcatcgct cgtaaaaagg actgggaccc gaaaaagtac
     4441 ggtggcttcg atagccctac agttgcctat tctgtcctag tagtggcaaa agttgagaag
     4501 ggaaaatcca agaaactgaa gtcagtcaaa gaattattgg ggataacgat tatggagcgc
     4561 tcgtcttttg aaaagaaccc catcgacttc cttgaggcga aaggttacaa ggaagtaaaa
     4621 aaggatctca taattaaact accaaagtat agtctgtttg agttagaaaa tggccgaaaa
     4681 cggatgttgg ctagcgccgg agagcttcaa aaggggaacg aactcgcact accgtctaaa
     4741 tacgtgaatt tcctgtattt agcgtcccat tacgagaagt tgaaaggttc acctgaagat
     4801 aacgaacaga agcaactttt tgttgagcag cacaaacatt atctcgacga aatcatagag
     4861 caaatttcgg aattcagtaa gagagtcatc ctagctgatg ccaatctgga caaagtatta
     4921 agcgcataca acaagcacag ggataaaccc atacgtgagc aggcggaaaa tattatccat
     4981 ttgtttactc ttaccaacct cggcgctcca gccgcattca agtattttga cacaacgata
     5041 gatcgcaaac gatacacttc taccaaggag gtgctagacg cgacactgat tcaccaatcc
     5101 atcacgggat tatatgaaac tcggatagat ttgtcacagc ttgggggtga ctctggtggt
     5161 tctactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt tatccaggaa
     5221 tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat
     5281 atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct tctgactagc
     5341 gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg tgagaacaag
     5401 attaagatgc tctctggtgg ttctcccaag aagaagagga aagtctaacc ggtcatcatc
     5461 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5521 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5581 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5641 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5701 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     5761 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     5881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7921 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     7981 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8041 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     8101 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     8161 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     8221 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     8281 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8341 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8401 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
