LOCUS       sequence-189552-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human PRDX5-WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pLD-puro-Cc-PRDX5WT-VA
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     467..486
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(508..1018)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(571..589)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    1067..1184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1232..1297)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(1310..1330)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1343..1363)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1373..1390)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1403..1426)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(1463..1486)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    1566..1590
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2236..2260)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2291..2315)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2312..2515)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2341..2361)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2804..2824)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(3122..3166)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3351..3584
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4077..4202
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4249..4429
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4430..4656)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4684..4704)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4684..4702)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4723..4739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4723..4739)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4736..4758)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4747..4763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4771..4777,4778..4795,4796..4801))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4816..4837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4954..4971)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5125..5713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5205..5224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5884..6675,6676..6744))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6507..6526
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6745..6849)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6875..7330)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7012..7033)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7224..7243
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7457..7479
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7471..7488
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7472..7488
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7498..7517
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7498..7516
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7537..7672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7712..7833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7723..7742)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7777..7796
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7905..8138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(8266..8379,1..486))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8349..8369)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
       61 ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca
      121 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
      181 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
      241 gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt
      301 gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat
      361 gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
      421 ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
      481 ggtcatggta agctccggtg gatccccctg gggagagagg tcggtgattc ggtcaacgag
      541 ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag
      601 gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct
      661 gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc
      721 tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg cggcttccgt
      781 ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg
      841 aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga
      901 cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga
      961 gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaaccccct
     1021 cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     1081 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     1141 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
     1201 ccatttgtct cgaggtcgag aattcgaact acttgtcatc gtcatccttg taatcgatgt
     1261 catgatcttt ataatcaccg tcatggtctt tgtagtctct tcctgcaggc ccctgaaagt
     1321 acaggttctc gctgcctcct ctcccctgga agtacagatt ttctcctgca gggtgatggt
     1381 gatggtgatg gccgcttccg cctttctcga actgcgggtg gctccagctt cctcctccgc
     1441 tgcctccgcc gcttcctcct cccttctcga actgagggtg gctccaggcg atcgccttga
     1501 tcaccacggg gggctggttc tcgcccagca ggttgaacag ctctctggcg atcgctctag
     1561 aatcaaccac tttgtacaag aaagttggca agagctgtga gatgatattg ggtgccaggc
     1621 tgcaggtgag gcctgtgcca tctggttcca cattcagggc cttcactatg ccatcctgta
     1681 ccaccatgga gaacctcttg agacgtcgat tcccaaagat ggacaccagc gaatcatcta
     1741 gtaataagtc tgtctccttc ccaaaggccc cagtgggatc agccaggagc cgaaccttgc
     1801 cttccgcctt gtgggctcgg ccccactcgc cagtcacaaa ggcatcatta acactcagac
     1861 aggccaccac ctggactccc ttggccttca gagcctcagc ctgctccaca aaccctggca
     1921 ggtgtgtctt ggaacatcca ggggtgaagg ccccaggaac tccaaacagc acacccttct
     1981 tgcccttgaa cagctctgcc aggttcacct tgttccctgg ctccccttca aacacctcca
     2041 ctgctgggat ggcatctccc accttgattg gggccatggc tgcagcggct ctgctgaaac
     2101 tgcggacccc gccagacgcc cactctcctt cactgcaccg tcttgctgcc gctgccgcag
     2161 actgaccgcc ggccccaccg acgagtatat agcccgctga gcgtctcagg gcgcacacgc
     2221 cagctagtcc catggtgcca acttttttgt acaaacttgt acgcgtacag cgtggatggc
     2281 gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     2341 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     2401 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     2461 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     2521 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     2581 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     2641 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     2701 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
     2761 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     2821 cctcggtgaa gggggcggcc gctcgaggct agtctcgtga tcgataccgt cgagatccgt
     2881 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     2941 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     3001 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     3061 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     3121 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     3181 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     3241 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     3301 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     3361 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     3421 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     3481 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     3541 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     3601 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     3661 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     3721 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     3781 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     3841 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     3901 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     3961 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     4021 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     4081 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     4141 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     4201 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     4261 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     4321 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     4381 gttagccaga gagctcccag gctcagatct ggtctaacca gagagacccg tttatgtatc
     4441 gagctaggca cttaaataca atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc
     4501 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca
     4561 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca
     4621 tgttgcaaga ctacaagagt attgcataag actacattaa gcttgcagct ccagcttttg
     4681 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     4741 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4801 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4861 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4921 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4981 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5041 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5101 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5161 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5221 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5281 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5341 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5401 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5461 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5521 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5581 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5641 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5701 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5761 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5821 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5881 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5941 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6001 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6061 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6121 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6181 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6241 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6301 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6361 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6421 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6481 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6541 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6601 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6661 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6721 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6781 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6841 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     6901 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     6961 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7021 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     7081 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     7141 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     7201 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     7261 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     7321 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     7381 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     7441 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     7501 tacgactcac tatagggcga attgggtacg tccctaggcc tccaaaaaag cctcctcact
     7561 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     7621 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     7681 ttaggggcgg gatagctaga gccagacatg ataagataca ttgatgagtt tggacaaacc
     7741 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     7801 tttgtaacca ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt
     7861 tgcaagttat aaatactgaa taataagatg acatgaacta ctactgctag agattttcca
     7921 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     7981 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     8041 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag
     8101 cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa
     8161 gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg atgcatgggg
     8221 tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt
     8281 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     8341 gccgagccgc tcgtagaagg ggaggttgcg gggcgcgga
//
