LOCUS       sequence-189556-        2721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit receiver vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3736_HC_Amp_ccdB_receiver vector
FEATURES             Location/Qualifiers
     source          1..2721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             463..768
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     647..666
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      809..880
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      896..923
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1027..1056
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      1078..1665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1566..1585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1677..1704)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1728..2519,2520..2588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2351..2370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2589..2691)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(2692..2721,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa
       61 ttcgtcatag gagagacgca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      121 aatgcaggga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
      181 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
      241 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
      301 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
      361 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
      421 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
      481 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
      541 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
      601 ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
      661 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
      721 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataagc tgatagtgct
      781 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      901 accttcgggt gggcctttct gcgtttatat actagtagcg gccgtctcaa cgataacgaa
      961 ttcaagcttg atatcattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
     1021 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
     1081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
     1201 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1621 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
     1681 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta ccaatgctta
     1741 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1801 cccgtcgtgt agataactac gatgcgggag ggcttaccat ctggccccag tgctgcaatg
     1861 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccggg
     1921 agggccgagc gcagaagtga tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1981 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2041 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2101 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2161 ggtcctccga tcgttgccag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2221 gcactgcata attctcttac tgtcatgcca tccgtgagat gcttttctgt gactggtgag
     2281 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2341 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2401 cgttcttcgg ggcgtaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2461 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2521 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2581 atactcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2641 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag
     2701 cctccggtcg gaggcttttg a
//
