LOCUS       sequence-189566-        1879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector H-J.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2986 HC_Kan_conn_H-J
FEATURES             Location/Qualifiers
     source          1..1879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      221..252
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        253..355
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             356..1171
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(429..448)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1195..1222
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1234..1821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1314..1333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1843..1872)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttttaa cgcagccgtc tccaggctaa taacagcttc cggactctag aacatcccta
      121 caggtgatat cctcgtgctt gagacgaggc cgttatgacg aattctctag atatcgctca
      181 atactgacca tttaaatcat acctgacctc catagcagaa agtcaaaagc ctccgaccgg
      241 aggcttttga cttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
      301 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
      361 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
      421 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
      481 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
      541 caatgatgtt acagatgaga tggtcaggct aaactggctg acggaattta tgcctcttcc
      601 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
      661 agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
      721 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
      781 cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttgg
      841 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
      901 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
      961 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     1021 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     1081 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1141 gtttcacttg atgctcgatg agtttttcta atgagggccc aaatgtaatc acctggctca
     1201 ccttcgggtg ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1261 gcatcacaaa aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1681 atttggtatc tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga
     1741 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1801 cgcagaaaaa aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg
     1861 tgaaggtgag ccagtgagt
//
