LOCUS       sequence-189567-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human PRDX5-E80K in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pLD-puro-Cc-PRDX5E80K-VA
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     484..503
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(525..1035)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(588..606)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    1084..1201
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1249..1314)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(1327..1347)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1360..1380)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1390..1407)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1420..1443)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(1480..1503)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    1583..1607
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2253..2277)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2308..2332)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2329..2532)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2358..2378)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2821..2841)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(3139..3183)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3368..3601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4094..4219
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4266..4446
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4447..4673)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4701..4721)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4701..4719)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4740..4756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4740..4756)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4753..4775)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4764..4780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4788..4794,4795..4812,4813..4818))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4833..4854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4971..4988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5142..5730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5222..5241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5901..6692,6693..6761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6524..6543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6762..6866)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6892..7347)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7029..7050)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7241..7260
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7474..7496
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7488..7505
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7489..7505
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7515..7534
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7515..7533
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7554..7689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7729..7850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7740..7759)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7794..7813
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7922..8155
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(8283..8379,1..503))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(join(8366..8379,1..7))
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc
       61 actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagacgccg
      121 acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc
      181 tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg
      241 aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg
      301 tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc
      361 tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg
      421 gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc
      481 accgtgggct tgtactcggt catggtaagc tccggtggat ccccctgggg agagaggtcg
      541 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg
      601 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc
      661 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc
      721 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg
      781 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga
      841 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg
      901 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc
      961 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
     1021 cgcaacccca accccctcga gccgcggcca aagtggatct ctgctgtccc tgtaataaac
     1081 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     1141 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     1201 attgtggatg aatactgcca tttgtctcga ggtcgagaat tcgaactact tgtcatcgtc
     1261 atccttgtaa tcgatgtcat gatctttata atcaccgtca tggtctttgt agtctcttcc
     1321 tgcaggcccc tgaaagtaca ggttctcgct gcctcctctc ccctggaagt acagattttc
     1381 tcctgcaggg tgatggtgat ggtgatggcc gcttccgcct ttctcgaact gcgggtggct
     1441 ccagcttcct cctccgctgc ctccgccgct tcctcctccc ttctcgaact gagggtggct
     1501 ccaggcgatc gccttgatca ccacgggggg ctggttctcg cccagcaggt tgaacagctc
     1561 tctggcgatc gctctagaat caaccacttt gtacaagaaa gttggcaaga gctgtgagat
     1621 gatattgggt gccaggctgc aggtgaggcc tgtgccatct ggttccacat tcagggcctt
     1681 cactatgcca tcctgtacca ccatggagaa cctcttgaga cgtcgattcc caaagatgga
     1741 caccagcgaa tcatctagta ataagtctgt ctccttccca aaggccccag tgggatcagc
     1801 caggagccga accttgcctt ccgccttgtg ggctcggccc cactcgccag tcacaaaggc
     1861 atcattaaca ctcagacagg ccaccacctg gactcccttg gccttcagag cctcagcctg
     1921 ctccacaaac cctggcaggt gtgtcttgga acatccaggg gtgaaggccc caggaactcc
     1981 aaacagcaca cccttcttgc ccttgaacag ctttgccagg ttcaccttgt tccctggctc
     2041 cccttcaaac acctccactg ctgggatggc atctcccacc ttgattgggg ccatggctgc
     2101 agcggctctg ctgaaactgc ggaccccgcc agacgcccac tctccttcac tgcaccgtct
     2161 tgctgccgct gccgcagact gaccgccggc cccaccgacg agtatatagc ccgctgagcg
     2221 tctcagggcg cacacgccag ctagtcccat ggtgccaact tttttgtaca aacttgtacg
     2281 cgtacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat
     2341 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     2401 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     2461 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     2521 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     2581 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     2641 aatagggggc gtacttggca tatgatagtc cattttaaaa cataatttta aaactgcaaa
     2701 ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac
     2761 agtcaaatta attccaatta tctctctaac agccttgtat cgtatatgca aatatgaagg
     2821 aatcatggga aataggccct cggtgaaggg ggcggccgct cgaggctagt ctcgtgatcg
     2881 ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
     2941 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
     3001 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     3061 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     3121 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     3181 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     3241 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     3301 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     3361 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
     3421 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
     3481 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
     3541 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
     3601 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
     3661 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
     3721 catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt
     3781 tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt
     3841 gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg
     3901 tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca
     3961 ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt
     4021 tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc
     4081 cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa
     4141 tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc
     4201 gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc
     4261 gccactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     4321 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     4381 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     4441 agacccgttt atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc
     4501 agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta
     4561 ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg
     4621 ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct
     4681 tgcagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     4741 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4801 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4861 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4921 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4981 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5041 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5101 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5161 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5221 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5281 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5341 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5401 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5461 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5521 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5581 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5641 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5701 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5761 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5821 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5881 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5941 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6001 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6061 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6121 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6181 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6241 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6301 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6361 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6421 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6481 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6541 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6601 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6661 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6721 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6781 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6841 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     6901 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     6961 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     7021 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     7081 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     7141 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     7201 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     7261 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     7321 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     7381 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     7441 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     7501 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacgtcc ctaggcctcc
     7561 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     7621 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     7681 gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata agatacattg
     7741 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     7801 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc
     7861 tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta
     7921 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     7981 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     8041 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     8101 ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc
     8161 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     8221 ggtaccgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
     8281 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     8341 tcggcggtga cggtgaagcc gagccgctcg tagaagggg
//
