LOCUS       sequence-189568-        1873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector Ha-J.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3734 HC_Kan_conn_Ha-J
FEATURES             Location/Qualifiers
     source          1..1873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(97..116)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     terminator      215..246
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        247..349
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             350..1165
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(423..442)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1189..1216
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1228..1815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1308..1327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1837..1866)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttttaa cgcagccgtc tccatccgag gctaactgaa acacggaagg agacaataca
      121 ggaaggaact gcttgagacg aggccgttat gacgaattct ctagatatcg ctcaatactg
      181 accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga ccggaggctt
      241 ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
      301 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat
      361 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata
      421 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
      481 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      541 tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc ttccgaccat
      601 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa
      661 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      721 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacggcga
      781 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag
      841 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
      901 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
      961 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     1021 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     1081 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1141 cttgatgctc gatgagtttt tctaatgagg gcccaaatgt aatcacctgg ctcaccttcg
     1201 ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1261 caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1681 tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc
     1741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1801 aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg
     1861 tgagccagtg agt
//
