LOCUS       sequence-189570-        1861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector H-Ha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1913 HC_Kan_conn_H-Ha
FEATURES             Location/Qualifiers
     source          1..1861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      204..233
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      255..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(854..881)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(905..1720)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1628..1647
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1721..1823)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1824..1855)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac
       61 gtctcgaggc taataacagc ttccggactc tagaacatcc ctacaggtga tatcctcgat
      121 cccgagacga aaacgaattc aagcttgata tcattcagga cgagcctcag actccagcgt
      181 aactggactg caatcaactc actggctcac cttcacgggt gggcctttct tcggtagaaa
      241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcat cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcagaa aggcccaccc gaaggtgagc caggtgatta catttgggcc
      901 ctcattagaa aaactcatcg agcatcaagt gaaactgcaa tttattcata tcaggattat
      961 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     1021 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     1081 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     1141 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     1201 gccagccatt acgctcgtca tcaaaatcac tcgcaccaac caaaccgtta ttcattcgtg
     1261 attgcgcctg agcgagacga aatacgcgat cgccgttaaa aggacaatta caaacaggaa
     1321 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     1381 gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg
     1441 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     1501 agtttagcct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     1561 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     1621 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     1681 gcggcctcga gcaagacgtt tcccgttgaa tatggctcat tttagcttcc ttagctcctg
     1741 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1801 tggaacctct tacgtgccga tcaagtcaaa agcctccggt cggaggcttt tgactttctg
     1861 c
//
