LOCUS       sequence-189573-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human PRDX5-K85R in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pLD-puro-Cc-PRDX5K85R-VA
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..248
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             459..692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    764..885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(801..820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     855..874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      925..1060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1080..1099)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1081..1099)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1109..1126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1109..1125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1118..1140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1267..1722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1354..1373)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1564..1585
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1748..1852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1853..1921,1922..2713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2071..2090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2884..3472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3373..3392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3626..3643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3760..3781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3796..3801,3802..3819,3820..3826)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3834..3850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3839..3861
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3858..3874
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3858..3874
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3893..3913
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3895..3913
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3941..4167
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4168..4348
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4395..4520
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5013..5246
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5431..5475
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     5773..5793
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        6082..6285
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6236..6256
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6282..6306
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6337..6361
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7007..7031)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7111..7134
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7171..7194
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7207..7224
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7234..7254
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7267..7287
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7300..7365
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7413..7530
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7579..8089
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8008..8026
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(8111..8379,1..331)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8111..8130)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
       61 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
      121 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
      181 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
      241 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
      301 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
      361 cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc
      421 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
      481 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
      541 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      601 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      661 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
      721 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
      781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      841 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
      901 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      961 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     1021 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     1081 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     1141 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1201 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1261 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1321 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1381 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1441 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1501 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1561 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1621 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1681 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2101 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2161 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2221 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2281 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2341 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2401 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2461 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2521 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2581 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2641 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2701 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3001 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3661 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3721 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3781 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3841 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     3901 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     3961 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     4021 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     4081 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     4141 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     4201 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4261 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4321 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     4381 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     4441 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     4501 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     4561 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     4621 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     4681 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     4741 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     4801 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     4861 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     4921 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     4981 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     5041 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     5101 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     5161 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     5221 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     5281 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     5341 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     5401 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     5461 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     5521 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     5581 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     5641 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     5701 tccattcgat tagtgaacgg atctcgacgg tatcgatcac gagactagcc tcgagcggcc
     5761 gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat acgatacaag
     5821 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     5881 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     5941 atggactatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     6001 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     6061 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     6121 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     6181 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     6241 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     6301 cagatcgcct ggagacgcca tccacgctgt acgcgtacaa gtttgtacaa aaaagttggc
     6361 accatgggac tagctggcgt gtgcgccctg agacgctcag cgggctatat actcgtcggt
     6421 ggggccggcg gtcagtctgc ggcagcggca gcaagacggt gcagtgaagg agagtgggcg
     6481 tctggcgggg tccgcagttt cagcagagcc gctgcagcca tggccccaat caaggtggga
     6541 gatgccatcc cagcagtgga ggtgtttgaa ggggagccag ggaacaaggt gaacctggca
     6601 gagctgttca agggcaggaa gggtgtgctg tttggagttc ctggggcctt cacccctgga
     6661 tgttccaaga cacacctgcc agggtttgtg gagcaggctg aggctctgaa ggccaaggga
     6721 gtccaggtgg tggcctgtct gagtgttaat gatgcctttg tgactggcga gtggggccga
     6781 gcccacaagg cggaaggcaa ggttcggctc ctggctgatc ccactggggc ctttgggaag
     6841 gagacagact tattactaga tgattcgctg gtgtccatct ttgggaatcg acgtctcaag
     6901 aggttctcca tggtggtaca ggatggcata gtgaaggccc tgaatgtgga accagatggc
     6961 acaggcctca cctgcagcct ggcacccaat atcatctcac agctcttgcc aactttcttg
     7021 tacaaagtgg ttgattctag agcgatcgcc agagagctgt tcaacctgct gggcgagaac
     7081 cagccccccg tggtgatcaa ggcgatcgcc tggagccacc ctcagttcga gaagggagga
     7141 ggaagcggcg gaggcagcgg aggaggaagc tggagccacc cgcagttcga gaaaggcgga
     7201 agcggccatc accatcacca tcaccctgca ggagaaaatc tgtacttcca ggggagagga
     7261 ggcagcgaga acctgtactt tcaggggcct gcaggaagag actacaaaga ccatgacggt
     7321 gattataaag atcatgacat cgattacaag gatgacgatg acaagtagtt cgaattctcg
     7381 acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     7441 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     7501 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     7561 ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
     7621 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     7681 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     7741 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     7801 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     7861 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     7921 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     7981 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     8041 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc
     8101 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     8161 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     8221 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     8281 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     8341 cacgccggag agcgtcgaag cgggggcggt gttcgccga
//
