LOCUS       sequence-189575-        1942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector L-N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2718 HC_Kan_conn_L-N
FEATURES             Location/Qualifiers
     source          1..1942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      283..312
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      334..921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     822..841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(933..960)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(984..1799)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1707..1726
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1800..1902)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1903..1934)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa ttcgtcagaa
       61 gttggtgagt tcagagcgtc tcacgtctat gatgaggatg ttggtggaga gcatgtggag
      121 gaagtggata gggaaggttg tagagtagat ccggttgaag tgatgaggat aggaggaggc
      181 taccgagacg gtgaatgagg agattgagct aacgaattca agcttgatat cattcaggac
      241 gagcctcaga ctccagcgta actggactgc aatcaactca ctggctcacc ttcacgggtg
      301 ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      421 gctaccaact ctttttccga ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      841 tatagtcctg tcgggtttcg ccacctctga cttgagcatc gatttttgtg atgctcgtca
      901 ggggggcgga gcctatggaa aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc
      961 aggtgattac atttgggccc tcattagaaa aactcatcga gcatcaagtg aaactgcaat
     1021 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     1081 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     1141 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     1201 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     1261 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcaccaacc
     1321 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gccgttaaaa
     1381 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     1441 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt ccctgggatc
     1501 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     1561 ggcataaatt ccgtcagcca gtttagcctg accatctcat ctgtaacatc attggcaacg
     1621 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     1681 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     1741 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc
     1921 ggaggctttt gactttctgc ta
//
