LOCUS       sequence-189580-        1923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector N-R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3761 HC_Kan_conn_N-R
FEATURES             Location/Qualifiers
     source          1..1923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      265..296
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        297..399
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             400..1215
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(473..492)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1239..1266
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1278..1865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1358..1377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1887..1916)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttttaa cgcagccgtc tccctacgac ataatccacc atcaacatgc aagtccagtc
      121 aacaactttc cctcagacgt tagtggtaat cgaacctcaa cacctagccc agaacaagcc
      181 ggttgagacg aggccgttat gacgaattct ctagatatcg ctcaatactg accatttaaa
      241 tcatacctga cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat
      301 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      361 tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa
      421 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
      481 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
      541 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
      601 gagatggtca ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
      661 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc
      721 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
      781 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt
      841 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat
      901 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
      961 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1021 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1081 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1141 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc
     1201 gatgagtttt tctaatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt
     1261 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1321 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1741 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1861 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg tgagccagtg
     1921 agt
//
