LOCUS       sequence-189585-        1849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector U-V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3757 HC_Kan_conn_U-V
FEATURES             Location/Qualifiers
     source          1..1849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      192..221
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      243..830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     731..750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(842..869)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(893..1708)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1616..1635
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1709..1811)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1812..1843)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac
       61 gtctcgtgga taatccaaca accccataac acccgccaca catacagttg cgagacgaaa
      121 acgaattcaa gcttgatatc attcaggacg agcctcagac tccagcgtaa ctggactgca
      181 atcaactcac tggctcacct tcacgggtgg gcctttcttc ggtagaaaat caaaggatct
      241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgag gtaactggct
      361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      781 ttgagcatcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      841 acgcagaaag gcccacccga aggtgagcca ggtgattaca tttgggccct cattagaaaa
      901 actcatcgag catcaagtga aactgcaatt tattcatatc aggattatca ataccatatt
      961 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     1021 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     1081 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     1141 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     1201 gctcgtcatc aaaatcactc gcaccaacca aaccgttatt cattcgtgat tgcgcctgag
     1261 cgagacgaaa tacgcgatcg ccgttaaaag gacaattaca aacaggaatc gaatgcaacc
     1321 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     1381 atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag
     1441 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagcctga
     1501 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     1561 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     1621 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc
     1681 aagacgtttc ccgttgaata tggctcattt tagcttcctt agctcctgaa aatctcgata
     1741 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     1801 cgtgccgatc aagtcaaaag cctccggtcg gaggcttttg actttctgc
//
