LOCUS       sequence-189587-        1944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector W-Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2733 HC_Kan_conn_W-Y
FEATURES             Location/Qualifiers
     source          1..1944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      284..313
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      335..922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     823..842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(934..961)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(985..1800)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1708..1727
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1801..1903)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1904..1935)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggaggtcagg tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtcactat
       61 cctaacgtcc acttcagcgt ctcacgaaca catattgatc ctcgtcacaa ccacaccagc
      121 acacctcaaa tcccacacca ctcccacaat taccattcac tcaacaaact cacacatccc
      181 actgcgagac gcaggtggat gaaagcgtgt aaacgaattc aagcttgata tcattcagga
      241 cgagcctcag actccagcgt aactggactg caatcaactc actggctcac cttcacgggt
      301 gggcctttct tcggtagaaa atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
      361 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      421 agctaccaac tctttttccg aggtaactgg cttcagcaga gcgcagatac caaatactgt
      481 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      841 ttatagtcct gtcgggtttc gccacctctg acttgagcat cgatttttgt gatgctcgtc
      901 aggggggcgg agcctatgga aaaacgccag caacgcagaa aggcccaccc gaaggtgagc
      961 caggtgatta catttgggcc ctcattagaa aaactcatcg agcatcaagt gaaactgcaa
     1021 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     1081 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     1141 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     1201 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     1261 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcaccaac
     1321 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgccgttaaa
     1381 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     1441 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat
     1501 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     1561 aggcataaat tccgtcagcc agtttagcct gaccatctca tctgtaacat cattggcaac
     1621 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     1681 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     1741 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat
     1801 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1861 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaagtcaaa agcctccggt
     1921 cggaggcttt tgactttctg ctat
//
