LOCUS       sequence-189588-        1905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector W-X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3159 HC_Kan_conn_W-X
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      246..275
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      297..884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     785..804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(896..923)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(947..1762)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1670..1689
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1763..1865)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1866..1897)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa ttcgtcacgt
       61 ctcgcgaatc ttgtattcag tctcgcactt tagacatggt cttctgctgc ccgtggtcct
      121 ctattcgtcg gatgttctgt cgattcttcc tgtctatctg cacgcgagac gaaaacgaat
      181 tcaagcttga tatcattcag gacgagcctc agactccagc gtaactggac tgcaatcaac
      241 tcactggctc accttcacgg gtgggccttt cttcggtaga aaatcaaagg atcttcttga
      301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      361 gtggtttgtt tgccggatca agagctacca actctttttc cgaggtaact ggcttcagca
      421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      841 atcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcag
      901 aaaggcccac ccgaaggtga gccaggtgat tacatttggg ccctcattag aaaaactcat
      961 cgagcatcaa gtgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     1021 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     1081 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     1141 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     1201 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     1261 catcaaaatc actcgcacca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     1321 gaaatacgcg atcgccgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     1381 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     1441 ggaatgctgt tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     1501 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagc ctgaccatct
     1561 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     1621 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     1681 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg
     1741 tttcccgttg aatatggctc attttagctt ccttagctcc tgaaaatctc gataactcaa
     1801 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     1861 gatcaagtca aaagcctccg gtcggaggct tttgactttc tgcta
//
