LOCUS       sequence-189597-        2193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 14 of EMMA. Functional category: 
            Constitutional promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1762 HC_Kan_SV40p_p14
FEATURES             Location/Qualifiers
     source          1..2193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..405
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      256..391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     318..337
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     terminator      541..570
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      592..1179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1080..1099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1191..1218)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1242..2057)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1965..1984
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2058..2160)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2161..2192)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tctgctatgg aggtcaggta tgatttaaat ggtcagtatt gagcgatatc tagagaattc
       61 gtcacgtctc gctacgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      121 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      181 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      241 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      301 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
      361 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
      421 tgtatatcca ttttcggatc tgatcaagag acagggcaac gagacgaaaa cgaattcaag
      481 cttgatatca ttcaggacga gcctcagact ccagcgtaac tggactgcaa tcaactcact
      541 ggctcacctt cacgggtggg cctttcttcg gtagaaaatc aaaggatctt cttgagatcc
      601 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      661 ttgtttgccg gatcaagagc taccaactct ttttccgagg taactggctt cagcagagcg
      721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcatcga
     1141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcagaaagg
     1201 cccacccgaa ggtgagccag gtgattacat ttgggccctc attagaaaaa ctcatcgagc
     1261 atcaagtgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     1321 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     1381 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     1441 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     1501 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     1561 aaatcactcg caccaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     1621 acgcgatcgc cgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     1681 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     1741 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     1801 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagcctgac catctcatct
     1861 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     1921 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     1981 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc
     2041 cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     2101 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca
     2161 agtcaaaagc ctccggtcgg aggcttttga ctt
//
