LOCUS       sequence-189602-        1949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 25 of EMMA. Functional category: 
            Viral origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1891 HC_Kan_SV40-ORI_p25
FEATURES             Location/Qualifiers
     source          1..1949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      71..206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     133..152
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     terminator      292..321
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      343..930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     831..850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(942..969)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(993..1808)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1716..1735
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1809..1911)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1912..1943)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac
       61 gtctcgactg atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      121 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
      181 gtagtgagga ggcttttttg gaggccacga cgagacgaaa acgaattcaa gcttgatatc
      241 attcaggacg agcctcagac tccagcgtaa ctggactgca atcaactcac tggctcacct
      301 tcacgggtgg gcctttcttc ggtagaaaat caaaggatct tcttgagatc ctttttttct
      361 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      421 ggatcaagag ctaccaactc tttttccgag gtaactggct tcagcagagc gcagatacca
      481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcatcg atttttgtga
      901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcagaaag gcccacccga
      961 aggtgagcca ggtgattaca tttgggccct cattagaaaa actcatcgag catcaagtga
     1021 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     1081 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     1141 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     1201 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     1261 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     1321 gcaccaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     1381 ccgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     1441 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     1501 cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     1561 gtcggaagag gcataaattc cgtcagccag tttagcctga ccatctcatc tgtaacatca
     1621 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     1681 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     1741 aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata
     1801 tggctcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1861 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag
     1921 cctccggtcg gaggcttttg actttctgc
//
